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Rabbit Recombinant Monoclonal CNOT6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-CNOT6 antibody [EPR22022] (AB221151), expandable thumbnail
  • Western blot - Anti-CNOT6 antibody [EPR22022] (AB221151), expandable thumbnail
  • Western blot - Anti-CNOT6 antibody [EPR22022] (AB221151), expandable thumbnail
  • Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (AB221151), expandable thumbnail
  • Western blot - Anti-CNOT6 antibody [EPR22022] (AB221151), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA. The increase of ligand-dependent ESR1-mediated transcription is much smaller, if any. Mediates cell proliferation and cell survival and prevents cellular senescence.

Alternative names

CCR4, CCR4a, KIAA1194, CNOT6, CCR4-NOT transcription complex subunit 6, CCR4 carbon catabolite repression 4-like, Carbon catabolite repressor protein 4 homolog, Cytoplasmic deadenylase

Recommended products

Rabbit Recombinant Monoclonal CNOT6 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22022
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151), expandable thumbnail

    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

    The expression profile is consistent with what has been described in the literature (PMID: 21741365).

    All lanes: Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1: Human testis lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human ovary lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Human heart lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 63 kDa

    Observed band size: 60 kDa

    Exposure time: 37s

  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151), expandable thumbnail

    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

    All lanes: Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1: His-tagged human CNOT6 recombinant protein(aa1-210) at 0.002 µg with 5% NFDM/TBST

    Lane 2: His-tagged human CNOT6L recombinant protein(aa1-210) at 0.002 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa

    Exposure time: 3s

  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151), expandable thumbnail

    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The reactivity of the antibody with the target protein in the HeLa lysate was blocked by the inclusion of the peptide antigen.

    All lanes: Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HeLa whole cell lysate at 20 µg with CNOT6 Immunogen peptide

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 63 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151), expandable thumbnail

    Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

    CNOT6 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input).

    Lane 2: ab221151 IP in HeLa whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab221151 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

    Predicted band size: 36 kDa, 63 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151), expandable thumbnail

    Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CNOT6 antibody [EPR22022] (ab221151) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 4: Human ovary cancer lysate at 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lane 4: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 63 kDa

    Exposure time: 3min

  • Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151), expandable thumbnail

    Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

    CNOT6 was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221151 at 1/500 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/1000 dilution.

    Lane 1: Jurkat whole cell lysate 10 μg (Input).

    Lane 2: ab221151 IP in Jurkat whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab221151 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (ab221151)

    Predicted band size: 63 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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