Rabbit Recombinant Monoclonal CNOT6 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (AB243294), expandable thumbnail](https://content.abcam.com/products/images/cnot6-antibody-epr22022-bsa-and-azide-free-ab243294--immunoprecipitation-img4619.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (AB243294)")
![Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (AB243294), expandable thumbnail](https://content.abcam.com/products/images/cnot6-antibody-epr22022-bsa-and-azide-free-ab243294--immunoprecipitation-img167.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (AB243294)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. In the presence of ZNF335, enhances ligand-dependent transcriptional activity of nuclear hormone receptors, including RARA. The increase of ligand-dependent ESR1-mediated transcription is much smaller, if any. Mediates cell proliferation and cell survival and prevents cellular senescence.
Alternative names
CCR4, CCR4a, KIAA1194, CNOT6, CCR4-NOT transcription complex subunit 6, CCR4 carbon catabolite repression 4-like, Carbon catabolite repressor protein 4 homolog, Cytoplasmic deadenylase
Recommended products
Rabbit Recombinant Monoclonal CNOT6 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22022
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab243294 is the carrier-free version of Anti-CNOT6 antibody [EPR22022] ab221151.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (ab243294), expandable thumbnail](https://content.abcam.com/products/images/cnot6-antibody-epr22022-bsa-and-azide-free-ab243294--immunoprecipitation-img4619.jpg "Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (ab243294)")
Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (ab243294)
CNOT6 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-CNOT6 antibody [EPR22022] ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-CNOT6 antibody [EPR22022] ab221151 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: Anti-CNOT6 antibody [EPR22022] ab221151 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CNOT6 antibody [EPR22022] ab221151 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-CNOT6 antibody [EPR22022] ab221151).
All lanes: Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (Anti-CNOT6 antibody [EPR22022] ab221151)
Predicted band size: 36 kDa, 63 kDa
Observed band size: 38 kDa
![Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (ab243294), expandable thumbnail](https://content.abcam.com/products/images/cnot6-antibody-epr22022-bsa-and-azide-free-ab243294--immunoprecipitation-img167.jpg "Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (ab243294)")
Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] - BSA and Azide free (ab243294)
CNOT6 was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with Anti-CNOT6 antibody [EPR22022] ab221151 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-CNOT6 antibody [EPR22022] ab221151 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate 10 μg (Input).
Lane 2: Anti-CNOT6 antibody [EPR22022] ab221151 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CNOT6 antibody [EPR22022] ab221151 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-CNOT6 antibody [EPR22022] ab221151).
All lanes: Immunoprecipitation - Anti-CNOT6 antibody [EPR22022] (Anti-CNOT6 antibody [EPR22022] ab221151)
Predicted band size: 63 kDa
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