Rabbit Polyclonal CNPase antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CNP.
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Publications
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- Disease models & mechanisms 16:2023Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiyun Shen,Shuxin Liu,Xiaojing Wei,Yaping Wang,Lin YangPubMed 36518009
- Glia 70:1652-16652022Cyclin-dependent kinase 7 contributes to myelin maintenance in the adult central nervous system and promotes myelin gene expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValérie Dion,Nathalie Schumacher,Nathalie Masar,Alexandra Pieltain,Pierre Tocquin,Pierre Lesoinne,Brigitte Malgrange,Renaud Vandenbosch,Rachelle FranzenPubMed 35488490
- Drug design, development and therapy 14:1377-13892020MsrA Suppresses Inflammatory Activation of Microglia and Oxidative Stress to Prevent Demyelination via Inhibition of the NOX2-MAPKs/NF-κB Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Fan et. al.PubMed 32308370
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CNP. The exact immunogen used to generate this antibody is proprietary information. Database link P09543
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Reactivity data
Select an application| WB | IHC-P | IHC-Fr | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Tested | Expected | Tested |
| Rat | Tested | Expected | Expected |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/10000 | Notes - |
Species Rat | Dilution info 1/500 - 1/10000 | Notes - |
Species Human | Dilution info 1/500 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Catalyzes the formation of 2'-nucleotide products from 2',3'-cyclic substrates (By similarity). May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin (By similarity).
Alternative names
CNP, CNPase
Recommended products
Rabbit Polyclonal CNPase antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CNP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CNP. The exact immunogen used to generate this antibody is proprietary information. Database link P09543
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
Western blot - Anti-CNPase antibody (ab227218)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-CNPase antibody (ab227218) at 1/5000 dilution
Lane 1: Mouse brain tissue extract at 50 µg
Lane 2: Rat brain tissue extract at 50 µg
Predicted band size: 48 kDa
Western blot - Anti-CNPase antibody (ab227218)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-CNPase antibody (ab227218) at 1/1000 dilution
All lanes: MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 48 kDa
Western blot - Anti-CNPase antibody (ab227218)
10% SDS-PAGE gel.
All lanes: Western blot - Anti-CNPase antibody (ab227218) at 1/500 dilution
Lane 1: WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg
Lane 2: CNPase knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody (ab227218)
Paraffin-embedded U87 (human) xenograft tissue stained for CNPase using ab227218 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Frozen sections) - Anti-CNPase antibody (ab227218)
Frozen sections of adult mouse cerebellum tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/500 dilution (red). Nuclear Counterstain: Fluoroshield with DAPI (blue).
Immunohistochemistry (Frozen sections) - Anti-CNPase antibody (ab227218)
Frozen sections of adult mouse hippocampus tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain: NeuN (red), stained by NeuN antibody at 1/500 dilution.
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