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AB227218

Anti-CNPase antibody

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(4 Publications)

Rabbit Polyclonal CNPase antibody. Suitable for ICC/IF, WB, IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CNP.

View Alternative Names

CNP, CNPase

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody (AB227218)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody (AB227218)

Paraffin-embedded U87 (human) xenograft tissue stained for CNPase using ab227218 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody (AB227218)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody (AB227218)

Immunofluorescence analysis of MOLT-4 cells fixed in 4% paraformaldehyde at RT for 15 min labelling CNPase protein at cell membrane using ab227218 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Immunohistochemistry (Frozen sections) - Anti-CNPase antibody (AB227218)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CNPase antibody (AB227218)

Frozen sections of adult mouse cerebellum tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain : beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/500 dilution (red). Nuclear Counterstain : Fluoroshield with DAPI (blue).

Immunohistochemistry (Frozen sections) - Anti-CNPase antibody (AB227218)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CNPase antibody (AB227218)

Frozen sections of adult mouse hippocampus tissue stained for CNPase (green) using ab227218 at 1/250 dilution in immunohistochemical analysis. Counterstain : NeuN (red), stained by NeuN antibody at 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody (AB227218)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNPase antibody (AB227218)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for CNPase protein at cell membrane with ab227218 at a 1/165 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Western blot - Anti-CNPase antibody (AB227218)
  • WB

Supplier Data

Western blot - Anti-CNPase antibody (AB227218)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-CNPase antibody (ab227218) at 1/500 dilution

Lane 1:

WT HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg

Lane 2:

CNPase knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 48 kDa

false

Western blot - Anti-CNPase antibody (AB227218)
  • WB

Supplier Data

Western blot - Anti-CNPase antibody (AB227218)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-CNPase antibody (ab227218) at 1/5000 dilution

Lane 1:

Mouse brain tissue extract at 50 µg

Lane 2:

Rat brain tissue extract at 50 µg

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IHC-Fr, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CNP. The exact immunogen used to generate this antibody is proprietary information.

P09543

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Alternative Products

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rhesus monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myelin-associated enzyme that catalyzes the phosphodiester hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides. In the mitochondria, regulates the functioning of the mitochondrial permeability transition pore (mPTP), and thus is involved in the mechanisms of cell death, both apoptosis and necrosis. Acts as an antiviral factor by suppressing the assembly of SARS-CoV-2 virions (PubMed : 38117830).. Acts as an antiviral factor by suppressing the assembly of HIV-1 virions through direct interaction with GAG.
See full target information CNP
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Disease models & mechanisms 16: PubMed36518009

2023

Infiltrating circulating monocytes provide an important source of BMP4 at the early stage of spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyun Shen,Shuxin Liu,Xiaojing Wei,Yaping Wang,Lin Yang

Journal of cellular physiology 237:3001-3011 PubMed35522572

2022

Adipose tissue-derived mesenchymal stem cells' acellular product extracellular vesicles as a potential therapy for Crohn's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Altemus,Neda Dadgar,Yan Li,Amy L Lightner

Glia 70:1652-1665 PubMed35488490

2022

Cyclin-dependent kinase 7 contributes to myelin maintenance in the adult central nervous system and promotes myelin gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Valérie Dion,Nathalie Schumacher,Nathalie Masar,Alexandra Pieltain,Pierre Tocquin,Pierre Lesoinne,Brigitte Malgrange,Renaud Vandenbosch,Rachelle Franzen

Drug design, development and therapy 14:1377-1389 PubMed32308370

2020

MsrA Suppresses Inflammatory Activation of Microglia and Oxidative Stress to Prevent Demyelination via Inhibition of the NOX2-MAPKs/NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Fan,Damiao Li,Xinlei Guan,Yanhui Yang,Junqiang Yan,Jian Shi,Ranran Ma,Qing Shu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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