Anti-CNPase antibody [EPR13005] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-CNPase antibody [EPR13005] - BSA and Azide free (AB250658)

This data was developed using ab183500, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CNPase antibody [EPR13005] (<a href='/en-us/products/primary-antibodies/cnpase-antibody-epr13005-ab183500'>ab183500</a>) at 1/1000 dilution

All lanes:

Human cerebellum lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CNPase antibody [EPR13005] - BSA and Azide free (AB250658)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab24560)

ab24560 staining Hippocalcin in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24560 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.