Anti-CNR2 antibody [HL3120] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CNR2 antibody [HL3120] - BSA and Azide free (AB325841)

ab325841 detects Cannabinoid receptor 2 protein by immunofluorescent analysis. Sample : Jurkat cells were fixed in ice-cold MeOH for 5 min. Green : ab325841 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-CNR2 antibody [HL3120] - BSA and Azide free (AB325841)

Boiled and unboiled Jurkat whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325841 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CNR2 antibody [HL3120] - BSA and Azide free (ab325841) at 1/1000 dilution

Lane 1:

Boiled Jurkat whole cell extracts at 30 µg

Lane 2:

Boiled Jurkat membrane extracts at 30 µg

Lane 3:

Unboiled Jurkat whole cell extracts at 30 µg

Lane 4:

Unboiled Jurkat membrane extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-CNR2 antibody [HL3120] - BSA and Azide free (AB325841)

Non-transfected (–) and transfected (+) unboiled Jurkat whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325841 diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CNR2 antibody [HL3120] - BSA and Azide free (ab325841) at 1/500 dilution

Lane 1:

Non-transfected (–) unboiled Jurkat whole cell extracts at 30 µg

Lane 2:

CNR2 shRNA transfected (+) unboiled Jurkat whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-CNR2 antibody [HL3120] - BSA and Azide free (AB325841)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325841 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CNR2 antibody [HL3120] - BSA and Azide free (ab325841) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

CNR2 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

CNR2 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false