AB46172

Anti-CNTF antibody

Name: Anti-CNTF antibody (ab46172) | Abcam
Brand: Abcam Limited
SKU: AB46172

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(13 Publications)

Rabbit Polyclonal CNTF antibody. Suitable for ELISA, WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CNTF.

View Alternative Names

Ciliary neurotrophic factor, CNTF

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-CNTF antibody (AB46172)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CNTF antibody (AB46172)

ICC/IF image of ab46172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46172, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Mouse

Applications

ELISA, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CNTF.

P26441

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

pH: 7 - 7.4 Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

See full target information CNTF

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26420 PubMed39488642

2024

Neurotrophic and synaptic effects of GnRH and/or GH upon motor function after spinal cord injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

C G Martínez-Moreno,D Calderón-Vallejo,C Díaz-Galindo,I Hernández-Jasso,J D Olivares-Hernández,J Ávila-Mendoza,D Epardo,J E Balderas-Márquez,V A Urban-Sosa,R Baltazar-Lara,M Carranza,M Luna,C Arámburo,J L Quintanar

Biology of the cell 115:e202300057 PubMed37851997

2023

Astrocytic phagocytosis of myelin debris and reactive characteristics in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Li,Zhibin Ding,Kexin Liu,Qing Wang,Lijuan Song,Zhi Chai,Jiezhong Yu,Dong Ma,Baoguo Xiao,Cungen Ma

Journal of Alzheimer's disease : JAD 82:631-646 PubMed34057082

2021

Neurotrophic Treatment Initiated During Early Postnatal Development Prevents the Alzheimer-Like Behavior and Synaptic Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Yinghua Liu,Chun-Ling Dai,Narjes Baazaoui,Yunn Chyn Tung,Fei Liu,Khalid Iqbal

Folia neuropathologica 59:67-80 PubMed33969678

2021

MiR-1b up-regulation inhibits rat neuron proliferation and regeneration yet promotes apoptosis via targeting KLF7.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Li,Lihe Yuan,Jin Wang,Zun Zhang,Shaojing Fu,Shaobin Wang,Xinhui Li

Neural regeneration research 15:503-511 PubMed31571662

2019

Protein microarray analysis of cytokine expression changes in distal stumps after sciatic nerve transection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Qing Cheng,Xue-Zhen Liang,Shuai Wei,Xiao Ding,Gong-Hai Han,Ping Liu,Xun Sun,Qi Quan,He Tang,Qing Zhao,Ai-Jia Shang,Jiang Peng

iScience 19:303-315 PubMed31404831

2019

CNTF and Nrf2 Are Coordinately Involved in Regulating Self-Renewal and Differentiation of Neural Stem Cell during Embryonic Development.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Peng Si,Guang Wang,Sha-Sha Han,Ya Jin,Yu-Xuan Hu,Mei-Yao He,Beate Brand-Saberi,Xuesong Yang,Guo-Sheng Liu

Molecular medicine reports 16:7534-7540 PubMed28944860

2017

Polyaniline promotes peripheral nerve regeneration by enhancement of the brain‑derived neurotrophic factor and ciliary neurotrophic factor expression and activation of the ERK1/2/MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Fan,Yan Xiong,Zhen Fu,Dingfeng Xu,Lei Wang,Yong Chen,Haoyang Xia,Na Peng,Shaojun Ye,Yanfeng Wang,Lina Zhang,Qifa Ye

Scientific reports 7:7079 PubMed28765641

2017

Hypomethylation of CNTFRα is associated with proliferation and poor prognosis in lower grade gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Fan,Xiaowen Wang,Jingwen Zhang,Romela Irene Ramos,Haibo Zhang,Chunjie Li,Dan Ye,Jiansheng Kang,Diego M Marzese,Dave S B Hoon,Wei Hua

International journal of obesity (2005) 41:149-158 PubMed27773938

2016

High fat induces acute and chronic inflammation in the hypothalamus: effect of high-fat diet, palmitate and TNF-α on appetite-regulating NPY neurons.

Applications

Unspecified application

Species

Unspecified reactive species

P S Dalvi,J A Chalmers,V Luo,D-Yd Han,L Wellhauser,Y Liu,D Q Tran,J Castel,S Luquet,M B Wheeler,D D Belsham

Neuroscience 301:338-50 PubMed26079333

2015

Congenital absence of corticospinal tract does not severely affect plastic changes of the developing postnatal spinal cord.

Applications

WB, IHC-P

Species

Mouse, Mouse

L Huang,Q Xian,N Shen,L Shi,Y Qu,L Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CNTF antibody

Immunocytochemistry/ Immunofluorescence - Anti-CNTF antibody (AB46172)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (13)

Product promise