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Rabbit Recombinant Monoclonal CNTF antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse samples.

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Images

Western blot - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (AB282123), expandable thumbnail
  • Western blot - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (AB282123), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (AB282123), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (AB282123), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (AB282123), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)IPWBIHC-FrIHC-P
Mouse
Expected
Tested
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Tested
Tested

Tested
Tested

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species

Rat

Dilution info

-

Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal CNTF antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR24553-104

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

ab282123 is the carrier-free version of Anti-CNTF antibody [EPR24553-104] ab270992.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Western blot - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Western blot - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Bands at 37, 50KD are likely to be multimer forms of CNTF (PMID: 9236223).

    Negative control: Brain, stomach (PMID: 12855189).

    Exposure time: 59 seconds.

    All lanes: Western blot - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992) at 1/1000 dilution

    Lane 1: Mouse sciatic nerve lysate at 20 µg

    Lane 2: Mouse brain lysate at 20 µg

    Lane 3: Mouse stomach lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 23 kDa

    Observed band size: 22 kDa, 37 kDa, 50 kDa

  • Western blot - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Western blot - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Stomach (PMID: 12855189).

    Exposure time: 81 seconds.

    All lanes: Western blot - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992) at 1/1000 dilution

    Lane 1: Rat sciatic nerve lysate at 20 µg

    Lane 2: Rat spinal cord lysate at 20 µg

    Lane 3: Rat stomach lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 23 kDa

    Observed band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse sciatic nerve tissue labelling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/500 dilution (0.962 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in mouse sciatic nerve. The section was incubated with Anti-CNTF antibody [EPR24553-104] ab270992 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat sciatic nerve tissue labelling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/500 dilution (0.962 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in rat sciatic nerve. The section was incubated with Anti-CNTF antibody [EPR24553-104] ab270992 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/500 dilution (0.962 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in mouse stomach. The section was incubated with Anti-CNTF antibody [EPR24553-104] ab270992 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse stomach (PMID: 12855189) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    CNTF was immunoprecipitated from 0.35 mg Mouse sciatic nerve lysate with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CNTF antibody [EPR24553-104] ab270992 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse sciatic nerve lysate 10ug

    Lane 2: Anti-CNTF antibody [EPR24553-104] ab270992 IP in Mouse sciatic nerve lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CNTF antibody [EPR24553-104] ab270992 in mouse sciatic nerve lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Bands at 37, 50KD are likely to be multimer forms of CNTF (PMID: 9236223).

    All lanes: Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992)

    Predicted band size: 23 kDa

    Observed band size: 22 kDa, 37 kDa, 50 kDa

  • Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    CNTF was immunoprecipitated from 0.35 mg Rat sciatic nerve lysate with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CNTF antibody [EPR24553-104] ab270992 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Rat sciatic nerve lysate 10ug

    Lane 2: Anti-CNTF antibody [EPR24553-104] ab270992 IP in Rat sciatic nerve lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CNTF antibody [EPR24553-104] ab270992 in rat sciatic nerve lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    Bands at 40, 75KD are likely to be multimer forms of CNTF (PMID: 9236223).

    All lanes: Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992)

    Predicted band size: 23 kDa

    Observed band size: 22 kDa, 37 kDa, 50 kDa

  • Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CNTF antibody [EPR24553-104] - BSA and Azide free (ab282123)

    This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat stomach tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on rat stomach (PMID: 12855189) is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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