Rabbit Recombinant Monoclonal CNTF antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
Flow Cyt (Intra) | IP | WB | IHC-Fr | IHC-P | |
---|---|---|---|---|---|
Mouse | Expected | Tested | Tested | Tested | Tested |
Rat | Tested | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species Rat | Dilution info - | Notes Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
Ciliary neurotrophic factor, CNTF, Cntf
Rabbit Recombinant Monoclonal CNTF antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, WB, IHC-Fr, IHC-P and reacts with Rat, Mouse samples.
IgG
Rabbit
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR24553-104
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab282123 is the carrier-free version of Anti-CNTF antibody [EPR24553-104] ab270992.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Bands at 37, 50KD are likely to be multimer forms of CNTF (PMID: 9236223).
Negative control: Brain, stomach (PMID: 12855189).
Exposure time: 59 seconds.
All lanes: Western blot - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992) at 1/1000 dilution
Lane 1: Mouse sciatic nerve lysate at 20 µg
Lane 2: Mouse brain lysate at 20 µg
Lane 3: Mouse stomach lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 22 kDa, 37 kDa, 50 kDa
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: Stomach (PMID: 12855189).
Exposure time: 81 seconds.
All lanes: Western blot - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992) at 1/1000 dilution
Lane 1: Rat sciatic nerve lysate at 20 µg
Lane 2: Rat spinal cord lysate at 20 µg
Lane 3: Rat stomach lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 22 kDa
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse sciatic nerve tissue labelling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/500 dilution (0.962 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in mouse sciatic nerve. The section was incubated with Anti-CNTF antibody [EPR24553-104] ab270992 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat sciatic nerve tissue labelling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/500 dilution (0.962 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining in rat sciatic nerve. The section was incubated with Anti-CNTF antibody [EPR24553-104] ab270992 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/500 dilution (0.962 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in mouse stomach. The section was incubated with Anti-CNTF antibody [EPR24553-104] ab270992 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse sciatic nerve tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse stomach tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse stomach (PMID: 12855189) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
CNTF was immunoprecipitated from 0.35 mg Mouse sciatic nerve lysate with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CNTF antibody [EPR24553-104] ab270992 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse sciatic nerve lysate 10ug
Lane 2: Anti-CNTF antibody [EPR24553-104] ab270992 IP in Mouse sciatic nerve lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CNTF antibody [EPR24553-104] ab270992 in mouse sciatic nerve lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Bands at 37, 50KD are likely to be multimer forms of CNTF (PMID: 9236223).
All lanes: Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992)
Predicted band size: 23 kDa
Observed band size: 22 kDa, 37 kDa, 50 kDa
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
CNTF was immunoprecipitated from 0.35 mg Rat sciatic nerve lysate with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-CNTF antibody [EPR24553-104] ab270992 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat sciatic nerve lysate 10ug
Lane 2: Anti-CNTF antibody [EPR24553-104] ab270992 IP in Rat sciatic nerve lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CNTF antibody [EPR24553-104] ab270992 in rat sciatic nerve lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Bands at 40, 75KD are likely to be multimer forms of CNTF (PMID: 9236223).
All lanes: Immunoprecipitation - Anti-CNTF antibody [EPR24553-104] (Anti-CNTF antibody [EPR24553-104] ab270992)
Predicted band size: 23 kDa
Observed band size: 22 kDa, 37 kDa, 50 kDa
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat sciatic nerve tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat sciatic nerve is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using Anti-CNTF antibody [EPR24553-104] ab270992, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat stomach tissue labeling CNTF with Anti-CNTF antibody [EPR24553-104] ab270992 at 1/100 dilution (4.81 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on rat stomach (PMID: 12855189) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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