Anti-CNTFR antibody [EPR24672-166]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. No staining on rat kidney. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on mouse hypothalamus (PMID : 27445662). The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. No staining on mouse cardiac muscle. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the rhodopsin of mouse retina. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Immunohistochemical analysis of paraffin-embedded Rat retina tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the rhodopsin of rat retina. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on rat cerebrum (PMID : 25886143). The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• WB

Lab

Western blot - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Blocking and diluting buffer and concentration : 5% NFDM /TBST. ab181602 was used as a GAPDH loading control at 1/1000000 dilution. Exposure time : Lane 1 : 180 seconds Lane 2 : 20 seconds The sensitivity of ab305245 is lower than that of ab283682, we recommend customers to choose ab283682 when performing WB.

Lane 1:

Western blot - Anti-CNTFR antibody [EPR24672-25] (<a href='/en-us/products/primary-antibodies/cntfr-antibody-epr24672-25-ab305245'>ab305245</a>) at 1/1000 dilution

Lane 2:

Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa

false

• WB

Lab

Western blot - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID : 12424252).

Low expression : Heart (PMID : 1648265), Negative control : U-87 MG (PMID : 25118897).

Exposure time : Lanes 1-2 : 70 seconds; Lanes 3-4 : 15 seconds.

All lanes:

Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 41 kDa

Observed band size: 60-70 kDa

false

• WB

Lab

Western blot - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Low expression : Heart (PMID : 1648265)

Exposure time : Lanes 1-2 : 70 seconds; Lanes 3-5 : 48 seconds.

All lanes:

Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse cerebellum tissue lysate at 20 µg

Lane 4:

Mouse P240 hippocampus tissue lysate at 20 µg

Lane 5:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 41 kDa

Observed band size: 60-70 kDa

false

• WB

Lab

Western blot - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 70 seconds

All lanes:

Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 41 kDa

Observed band size: 60-70 kDa

false

• WB

Lab

Western blot - Anti-CNTFR antibody [EPR24672-166] (AB283682)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 2:

Neuro-2a whole cell lysate treated with PNGase F at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 41 kDa

Observed band size: 40 kDa,60 kDa

false