Anti-CNTFR antibody [EPR24672-166]

11 product images

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  Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST. The molecular weight observed is consistent with what has been described in the literature (PMID:12424252).
  

  Low expression: Heart (PMID:1648265), Negative control: U-87 MG (PMID:25118897).

  Exposure time: Lanes 1-2: 70 seconds; Lanes 3-4: 15 seconds.

  All lanes: Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

  Lane 1: Human brain tissue lysate at 20 µg

  Lane 2: Human heart tissue lysate at 20 µg

  Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

  Lane 4: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Predicted band size: 41 kDa

  Observed band size: 60-70 kDa

• 

  Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  Low expression: Heart (PMID: 1648265)

  Exposure time: Lanes 1-2: 70 seconds; Lanes 3-5: 48 seconds.

  All lanes: Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse heart tissue lysate at 20 µg

  Lane 3: Mouse cerebellum tissue lysate at 20 µg

  Lane 4: Mouse P240 hippocampus tissue lysate at 20 µg

  Lane 5: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Predicted band size: 41 kDa

  Observed band size: 60-70 kDa

• 

  Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  Exposure time: 70 seconds

  All lanes: Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

  Lane 1: Rat brain tissue lysate at 20 µg

  Lane 2: Rat cerebellum tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Predicted band size: 41 kDa

  Observed band size: 60-70 kDa

• 

  Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  Exposure time: 3 minutes

  All lanes: Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

  Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

  Lane 2: Neuro-2a whole cell lysate treated with PNGase F at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Predicted band size: 41 kDa

  Observed band size: 40 kDa, 60 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. No staining on mouse cardiac muscle. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. No staining on rat kidney. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the rhodopsin of mouse retina. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on mouse hypothalamus (PMID: 27445662). The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Immunohistochemical analysis of paraffin-embedded Rat retina tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on the rhodopsin of rat retina. The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling CNTFR with ab283682 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on rat cerebrum (PMID: 25886143). The section was incubated with ab283682 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

  Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• 

  Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682)

  CNTFR Western blot staining of SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate using rabbit Anti-CNTFR antibody

  Blocking and diluting buffer and concentration: 5% NFDM /TBST.
  Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/1000000 dilution.
  Exposure time:
  Lane 1: 180 seconds
  Lane 2: 20 seconds
  The sensitivity of Anti-CNTFR antibody [EPR24672-25] ab305245 is lower than that of ab283682, we recommend customers to choose ab283682 when performing WB.

  Lane 1: Western blot - Anti-CNTFR antibody [EPR24672-25] (Anti-CNTFR antibody [EPR24672-25] ab305245) at 1/1000 dilution

  Lane 2: Western blot - Anti-CNTFR antibody [EPR24672-166] (ab283682) at 1/1000 dilution

  All lanes: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 60 kDa