AB89333

Anti-CNTFR antibody [MM0190-10L29]

Name: Anti-CNTFR antibody [MM0190-10L29] (ab89333) | Abcam
Brand: Abcam Limited
SKU: AB89333
Price: 535 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Mouse Monoclonal CNTFR antibody. Carrier free. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CNTFR.

View Alternative Names

Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor subunit alpha, CNTFR-alpha, CNTFR

2 Images

Flow Cytometry - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

Flow Cyt

Unknown

Flow Cytometry - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

Overlay histogram showing SH-SY5Y cells stained with ab89333 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89333, 0.5μg/1x10⁶ cells ) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2 (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4μm) labelling CNTFR with ab89333. Tissue was treated with 15μg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0190-10L29

Isotype

IgG2

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CNTFR.

P26992

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/mouse-igg2a-kappa-monoclonal-mg2a-53-low-endotoxin-azide-free-isotype-control-ab18414'>ab18414</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "15 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to CNTF. The alpha subunit provides the receptor specificity. Receptor for heterodimeric neurotropic cytokine composed of CLCF1/CLC and CRLF1/CLF-1 (PubMed : 26858303). Acts as a receptor for the neuroprotective peptide humanin as part of a complex with IL6ST/GP130 and IL27RA/WSX1 (PubMed : 19386761).

See full target information CNTFR

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed36497399

2022

Ciliary Neurotrophic Factor Modulates Multiple Downstream Signaling Pathways in Prostate Cancer Inhibiting Cell Invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Tossetta,Sonia Fantone,Rosaria Gesuita,Gaia Goteri,Martina Senzacqua,Fabio Marcheggiani,Luca Tiano,Daniela Marzioni,Roberta Mazzucchelli

Cell and tissue research 390:113-129 PubMed35794391

2022

Modulation of matrix metalloproteases by ciliary neurotrophic factor in human placental development.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Tossetta,Sonia Fantone,Elena Marinelli Busilacchi,Nicoletta Di Simone,Stefano R Giannubilo,Giovanni Scambia,Antonio Giordano,Daniela Marzioni

European journal of histochemistry : EJH 64: PubMed33131268

2020

Ciliary neurotrophic factor (CNTF) and its receptor (CNTFRα) signal through MAPK/ERK pathway in human prostate tissues: a morphological and biomolecular study.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Fantone,Giovanni Tossetta,Rodolfo Montironi,Martina Senzacqua,Daniela Marzioni,Roberta Mazzucchelli

Cancer immunology research 8:1193-1205 PubMed32661094

2020

Chemotherapeutic Tumor Microparticles Elicit a Neutrophil Response Targeting Malignant Pleural Effusions.

Applications

Unspecified application

Species

Unspecified reactive species

Pingwei Xu,Ke Tang,Jingwei Ma,Huafeng Zhang,Dianheng Wang,Liyan Zhu,Jie Chen,Keke Wei,Jincheng Liu,Haiqing Fang,Liang Tang,Yi Zhang,Jing Xie,Yuying Liu,Rui Meng,Li Liu,Xiaorong Dong,Kunyu Yang,Gang Wu,Fei Ma,Bo Huang

Frontiers in endocrinology 10:768 PubMed31781039

2019

Biological Effects of Ciliary Neurotrophic Factor on hMADS Adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Perugini,Eleonora Di Mercurio,Giovanni Tossetta,Ilenia Severi,Federica Monaco,Marcella Reguzzoni,Marco Tomasetti,Christian Dani,Saverio Cinti,Antonio Giordano

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CNTFR antibody [MM0190-10L29]

Flow Cytometry - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CNTFR antibody [MM0190-10L29] (AB89333)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise