Rabbit Polyclonal COASY antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human COASY aa 200-300.
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Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human COASY aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13057
Reactivity data
Select an application| IP | |
|---|---|
| Human | Tested |
| Pig | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Bifunctional enzyme that catalyzes the fourth and fifth sequential steps of CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation.
Alternative names
Bifunctional coenzyme A synthase, CoA synthase, NBP, POV-2, COASY, PSEC0106
Recommended products
Rabbit Polyclonal COASY antibody. Suitable for IP and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human COASY aa 200-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human COASY aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13057
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab226400 was affinity purified using an epitope specific to COASY immobilized on solid support
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ab226400 has not performed satisfactorily when used for WB of COASY in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of COASY.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
1 product image
Immunoprecipitation - Anti-COASY antibody (ab226400)
COASY was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226400 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226400 at 1 μg/ml.
Lane 1: ab226400 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-COASY antibody (ab226400)
Predicted band size: 62 kDa
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