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AB129012

Anti-COASY antibody [EPR8246]

4

(1 Review)

|

(3 Publications)

Rabbit Recombinant Monoclonal COASY antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

PSEC0106, COASY, Bifunctional coenzyme A synthase, CoA synthase, NBP, POV-2

3 Images
Flow Cytometry (Intracellular) - Anti-COASY antibody [EPR8246] (AB129012)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-COASY antibody [EPR8246] (AB129012)

ab129012, at 1/10 dilution, staining COASY in permeabilized HeLa cells by intracellular flow cytometry (red) or a Rabbit IgG negative control (green).

Western blot - Anti-COASY antibody [EPR8246] (AB129012)
  • WB

Unknown

Western blot - Anti-COASY antibody [EPR8246] (AB129012)

All lanes:

Western blot - Anti-COASY antibody [EPR8246] (ab129012) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 62 kDa

false

OI-RD Scanning - Anti-COASY antibody [EPR8246] (AB129012)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-COASY antibody [EPR8246] (AB129012)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-COASY antibody [EPR8246] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8246

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Bifunctional enzyme that catalyzes the fourth step of the coenzyme A biosynthetic pathway, the adenylation of 4'-phosphopantetheine, and the fifth step, the phosphorylation of dephospho-CoA to CoA.
See full target information COASY

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical laboratory analysis 36:e24678 PubMed36036768

2022

ICAT promotes colorectal cancer metastasis via binding to JUP and activating the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zihan Wang,Jiancong Hu,Junxiong Chen,Jingdan Zhang,Weiqian Li,Yu Tian,Huanliang Liu,Xiangling Yang

Cancer research 80:334-346 PubMed31704889

2019

CoA Synthase () Mediates Radiation Resistance via PI3K Signaling in Rectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sylvain Ferrandon,Jennifer DeVecchio,Leonardo Duraes,Hanumant Chouhan,Georgios Karagkounis,Jacqueline Davenport,Matthew Orloff,David Liska,Matthew F Kalady

FASEB journal : official publication of the Federa 33:7863-7881 PubMed30939247

2019

Reduced mitochondrial lipid oxidation leads to fat accumulation in myosteatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan P Gumucio,Austin H Qasawa,Patrick J Ferrara,Afshan N Malik,Katsuhiko Funai,Brian McDonagh,Christopher L Mendias
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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