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AB96725

Anti-Coatomer subunit delta/COPD antibody

5

(1 Review)

|

(5 Publications)

Rabbit Polyclonal Coatomer subunit delta/COPD antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARCN1 aa 50-500.

View Alternative Names

COPD, ARCN1, Coatomer subunit delta, Archain, Delta-coat protein, Delta-COP

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (AB96725)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (AB96725)

Paraformaldehyde-fixed HeLa cells stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-tubulin antibody (red).

Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (AB96725)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (AB96725)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96725 at 1/200 dilution. Lower image merged with DNA probe.

Immunohistochemistry (Frozen sections) - Anti-Coatomer subunit delta/COPD antibody (AB96725)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Coatomer subunit delta/COPD antibody (AB96725)

Frozen section of mouse cerebellum tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/250 dilution (green). The nuclear counter stain is DAPI (blue). NF-H is detected with an anti NF-H antibody (red).

Antigen Retrieval : Citrate buffer, pH 6.0, 10 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coatomer subunit delta/COPD antibody (AB96725)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coatomer subunit delta/COPD antibody (AB96725)

Paraffin-embedded mouse brain tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 10 min.

Western blot - Anti-Coatomer subunit delta/COPD antibody (AB96725)
  • WB

Unknown

Western blot - Anti-Coatomer subunit delta/COPD antibody (AB96725)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Coatomer subunit delta/COPD antibody (ab96725) at 1/500 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-Fr, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARCN1 aa 50-500. The exact immunogen used to generate this antibody is proprietary information.

P48444

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Complementary Products

Primary Antibodies

AB2899

Anti-beta COP antibody

Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (AB2899)

  • 5
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB5176

Anti-Histone H3 (phospho S10) antibody

Western blot - Anti-Histone H3 (phospho S10) antibody (AB5176)

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Primary Antibodies

AB10158

Anti-Histone H4 antibody - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H4 antibody - ChIP Grade (AB10158)

  • 4
  • 21
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.</p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Coatomer subunit delta also called COPD is a component of the COPI coatomer complex involved in membrane trafficking processes. This protein has a mass of approximately 50 kDa and is found in Golgi-associated transport systems. It interacts with other coatomer proteins to form a multi-subunit complex that assists in transporting molecules between Golgi compartments and from the Golgi to the endoplasmic reticulum. Its presence is typically noted in most eukaryotic cells contributing to its essential role in the cellular transport machinery.
Biological function summary

The coatomer subunit delta participates as a part of the COPI coatomer complex which is essential in vesicle formation. This complex stabilizes curvature in membranes to help form vesicles important for intracellular transport. It is key in the recycling of proteins and lipids to the endoplasmic reticulum maintaining homeostasis within the cell. The coatomer complex works with proteins such as ARF1 which activates the assembly of the coatomer complex onto membranes facilitating proper cargo selection and vesicle budding.

Pathways

The coatomer subunit delta is integral to vesicular trafficking pathways including the retrograde transport pathway from Golgi to the endoplasmic reticulum. It plays a significant role in the secretory pathway which is central to the transport of proteins and lipids. It has interactions with other coatomer subunits and accessory proteins ensuring efficient pathway function. It relates closely to proteins such as ARF1 and various cargo receptors which are essential for sorting and transport of cargo.

Improper functioning of the coatomer subunit delta can contribute to neurodegenerative conditions and certain types of cancer. Its disturbance may relate to Alzheimer’s disease where cellular transport mechanisms are disrupted leading to protein misfolding and aggregation. Additionally abnormalities in coatomer subunits are linked to some cancers potentially due to their influence on cell growth and proliferation. The interactions with proteins like ARF1 can amplify these effects highlighting the necessity of functional coatomer subunit delta for health maintenance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the coatomer, a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).
See full target information ARCN1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Biochemical journal 480:1109-1127 PubMed37409526

2023

RhoGAP6 interacts with COPI to regulate protein transport.

Applications

Unspecified application

Species

Unspecified reactive species

Lorna O'Donoghue,Shane P Comer,Dishon W Hiebner,Ingmar Schoen,Alex von Kriegsheim,Albert Smolenski

Cell chemical biology 26:1380-1392.e6 PubMed31378711

2019

A Genome-wide Functional Signature Ontology Map and Applications to Natural Product Mechanism of Action Discovery.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A McMillan,Gino Kwon,Jean R Clemenceau,Kurt W Fisher,Rachel M Vaden,Anam F Shaikh,Beth K Neilsen,David Kelly,Malia B Potts,Yeo-Jin Sung,Saurabh Mendiratta,Suzie K Hight,Yunji Lee,John B MacMillan,Robert E Lewis,Hyun Seok Kim,Michael A White

American journal of human genetics 99:451-9 PubMed27476655

2016

ARCN1 Mutations Cause a Recognizable Craniofacial Syndrome Due to COPI-Mediated Transport Defects.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Izumi,Maggie Brett,Eriko Nishi,Séverine Drunat,Ee-Shien Tan,Katsunori Fujiki,Sophie Lebon,Breana Cham,Koji Masuda,Michiko Arakawa,Adeline Jacquinet,Yusuke Yamazumi,Shu-Ting Chen,Alain Verloes,Yuki Okada,Yuki Katou,Tomohiko Nakamura,Tetsu Akiyama,Pierre Gressens,Roger Foo,Sandrine Passemard,Ene-Choo Tan,Vincent El Ghouzzi,Katsuhiko Shirahige

Journal of virology 87:2673-85 PubMed23255804

2012

Dissecting the role of COPI complexes in influenza virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Eileen Sun,Jiang He,Xiaowei Zhuang

Proceedings of the National Academy of Sciences of 109:12485-90 PubMed22802617

2012

Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI.

Applications

WB

Species

Human

Xin Zhang,Wenchao Wang,Anne V Bedigian,Margaret L Coughlin,Timothy J Mitchison,Ulrike S Eggert
View all publications
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Product promise

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