Rabbit Polyclonal Coatomer subunit delta/COPD antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARCN1 aa 50-500.
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Publications
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- Cell chemical biology 26:1380-1392.e62019A Genome-wide Functional Signature Ontology Map and Applications to Natural Product Mechanism of Action Discovery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A McMillan et. al.PubMed 31378711
- American journal of human genetics 99:451-92016ARCN1 Mutations Cause a Recognizable Craniofacial Syndrome Due to COPI-Mediated Transport Defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKosuke Izumi et. al.PubMed 27476655
- Journal of virology 87:2673-852012Dissecting the role of COPI complexes in influenza virus infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEileen Sun,Jiang He,Xiaowei ZhuangPubMed 23255804
- Proceedings of the National Academy of Sciences of 109:12485-902012Dopamine receptor D3 regulates endocytic sorting by a Prazosin-sensitive interaction with the coatomer COPI.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARCN1 aa 50-500. The exact immunogen used to generate this antibody is proprietary information. Database link P48444
Reactivity data
Select an application| IHC-P | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Expected |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes Antigen Retrieval: Citrate buffer, pH 6.0, 10 min. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Component of the coatomer, a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).
Alternative names
COPD, ARCN1, Coatomer subunit delta, Archain, Delta-coat protein, Delta-COP
Recommended products
Rabbit Polyclonal Coatomer subunit delta/COPD antibody. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ARCN1 aa 50-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARCN1 aa 50-500. The exact immunogen used to generate this antibody is proprietary information. Database link P48444
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Coatomer subunit delta also called COPD is a component of the COPI coatomer complex involved in membrane trafficking processes. This protein has a mass of approximately 50 kDa and is found in Golgi-associated transport systems. It interacts with other coatomer proteins to form a multi-subunit complex that assists in transporting molecules between Golgi compartments and from the Golgi to the endoplasmic reticulum. Its presence is typically noted in most eukaryotic cells contributing to its essential role in the cellular transport machinery.
- Biological function summary
The coatomer subunit delta participates as a part of the COPI coatomer complex which is essential in vesicle formation. This complex stabilizes curvature in membranes to help form vesicles important for intracellular transport. It is key in the recycling of proteins and lipids to the endoplasmic reticulum maintaining homeostasis within the cell. The coatomer complex works with proteins such as ARF1 which activates the assembly of the coatomer complex onto membranes facilitating proper cargo selection and vesicle budding.
- Pathways
The coatomer subunit delta is integral to vesicular trafficking pathways including the retrograde transport pathway from Golgi to the endoplasmic reticulum. It plays a significant role in the secretory pathway which is central to the transport of proteins and lipids. It has interactions with other coatomer subunits and accessory proteins ensuring efficient pathway function. It relates closely to proteins such as ARF1 and various cargo receptors which are essential for sorting and transport of cargo.
- Associated diseases and disorders
Improper functioning of the coatomer subunit delta can contribute to neurodegenerative conditions and certain types of cancer. Its disturbance may relate to Alzheimer’s disease where cellular transport mechanisms are disrupted leading to protein misfolding and aggregation. Additionally abnormalities in coatomer subunits are linked to some cancers potentially due to their influence on cell growth and proliferation. The interactions with proteins like ARF1 can amplify these effects highlighting the necessity of functional coatomer subunit delta for health maintenance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Immunohistochemistry (Frozen sections) - Anti-Coatomer subunit delta/COPD antibody (ab96725)
Frozen section of mouse cerebellum tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/250 dilution (green). The nuclear counter stain is DAPI (blue). NF-H is detected with an anti NF-H antibody (red).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coatomer subunit delta/COPD antibody (ab96725)
Paraffin-embedded mouse brain tissue stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution in immunohistochemical analysis.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min.
Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (ab96725)
Paraformaldehyde-fixed HeLa cells stained for Coatomer subunit delta/COPD using ab96725 at 1/500 dilution (green) in ICC/IF. Alpha tubulin filaments are detected with an anti-tubulin antibody (red).
Western blot - Anti-Coatomer subunit delta/COPD antibody (ab96725)
7.5% SDS PAGE
All lanes: Western blot - Anti-Coatomer subunit delta/COPD antibody (ab96725) at 1/500 dilution
Lane 1: H1299 whole cell lysate at 30 µg
Lane 2: HepG2 whole cell lysate at 30 µg
Predicted band size: 57 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (ab96725)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96725 at 1/200 dilution. Lower image merged with DNA probe.
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