AB70564

Anti-CoCoA antibody

Name: Anti-CoCoA antibody (ab70564) | Abcam
Brand: Abcam Limited
SKU: AB70564
Price: 510 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal CoCoA antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human CALCOCO1.

View Alternative Names

KIAA1536, PP13275, UNQ2436/PRO4996, CALCOCO1, Calcium-binding and coiled-coil domain-containing protein 1, Calphoglin, Coiled-coil coactivator protein, Sarcoma antigen NY-SAR-3

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Immunoprecipitation - Anti-CoCoA antibody (AB70564)

Detection of human CoCoA by Immunoprecipitation in whole cell lysate from MCF7 cells (1 mg, 20% of immunoprecipitate loaded/lane), using ab70564 at 3 µg/mg of lysate (lane 2).
CoCoA was also immunoprecipitated using another CoCoA antibody (ab70563) which recognizes an upstream epitope (lane 1).
Lane 3 : Control IgG immunoprecipitate.
Subsequent blotting of immunoprecipitate for the detection of CoCoA was performed using 1µg/ml of ab70564 and 50µg of lysate.
Detection was by chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-CoCoA antibody (ab70564)

Predicted band size: 77 kDa

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Western blot - Anti-CoCoA antibody (AB70564)

All lanes:

Western blot - Anti-CoCoA antibody (ab70564) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

NIH3T3 whole cell lysate at 50 µg

Lane 4:

Ramos whole cell lysate at 50 µg

Lane 5:

MCF7 whole cell lysate at 50 µg

Predicted band size: 77 kDa

Observed band size: 90 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human CALCOCO1. The exact immunogen used to generate this antibody is proprietary information.

Q9P1Z2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab70564 was affinity purified using an epitope specific to CoCoA immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CoCoA also known as CCNDBP1 or Coactivator of Coactivator is a protein with a molecular mass of approximately 65 kDa. It acts as a transcription coactivator and interacts with other transcription factors to enhance gene expression. CoCoA is widely expressed in a variety of tissues suggesting a broad role in cellular processes. The protein’s expression in diverse cell types highlights its involvement in fundamental biological activities.

Biological function summary

CoCoA plays a significant role in the transcriptional regulation of genes. It functions as a part of multiprotein complexes where it assists in the enhancement of transcriptional activity by interacting with nuclear receptors and other coactivators. This interaction and formation of complexes allow CoCoA to efficiently fine-tune gene expression facilitating cell proliferation differentiation and various cellular responses. Its ability to modulate transcription makes CoCoA an important player in controlling the cell cycle and other vital cellular processes.

Pathways

Numerous cellular processes such as steroid hormone signaling and Wnt signaling pathways involve CoCoA. These pathways play a critical role in regulating developmental and physiological processes. CoCoA interacts with the estrogen receptor impacting the steroid hormone signaling pathway and it also ties with β-catenin in the Wnt signaling pathway. These interactions enable CoCoA to coordinate the transcription of specific target genes necessary for cellular development and homeostasis.

CoCoA has connections to cancer and neurodegenerative diseases. In the context of cancer altered CoCoA expression or function can influence tumor progression with changes linked to breast and prostate cancers. In these situations CoCoA affects pathways with proteins like estrogen receptor or androgen receptor. Regarding neurodegenerative diseases CoCoA may contribute through its regulatory roles in signaling pathways that include interactions with proteins known for neural function. Understanding CoCoA's role across these diseases may unveil potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Functions as a coactivator for aryl hydrocarbon and nuclear receptors (NR). Recruited to promoters through its contact with the N-terminal basic helix-loop-helix-Per-Arnt-Sim (PAS) domain of transcription factors or coactivators, such as NCOA2. During ER-activation acts synergistically in combination with other NCOA2-binding proteins, such as EP300, CREBBP and CARM1. Involved in the transcriptional activation of target genes in the Wnt/CTNNB1 pathway. Functions as a secondary coactivator in LEF1-mediated transcriptional activation via its interaction with CTNNB1. Coactivator function for nuclear receptors and LEF1/CTNNB1 involves differential utilization of two different activation regions (By similarity). In association with CCAR1 enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells (PubMed : 24245781).. Seems to enhance inorganic pyrophosphatase thus activating phosphogluomutase (PMG). Probably functions as a component of the calphoglin complex, which is involved in linking cellular metabolism (phosphate and glucose metabolism) with other core functions including protein synthesis and degradation, calcium signaling and cell growth.

See full target information CALCOCO1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 16:2219-2237 PubMed31971854

2020

Mass spectrometry proteomics reveals a function for mammalian CALCOCO1 in MTOR-regulated selective autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan A Stefely,Yu Zhang,Elyse C Freiberger,Nicholas W Kwiecien,Hala Elnakat Thomas,Alexander M Davis,Nathaniel D Lowry,Catherine E Vincent,Evgenia Shishkova,Nicholas A Clark,Mario Medvedovic,Joshua J Coon,David J Pagliarini,Carol A Mercer

Journal of lipid research 54:1776-85 PubMed23596326

2013

Identification of candidate genes encoding an LDL-C QTL in baboons.

Applications

Unspecified application

Species

Unspecified reactive species

Genesio M Karere,Jeremy P Glenn,Shifra Birnbaum,Sassan Hafizi,David L Rainwater,Michael C Mahaney,John L VandeBerg,Laura A Cox

View all publications

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Product promise

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Anti-CoCoA antibody

Immunoprecipitation - Anti-CoCoA antibody (AB70564)

Western blot - Anti-CoCoA antibody (AB70564)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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