Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
IHC-P | ICC/IF | WB | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Expected | Expected |
Rat | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 1/100 - 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/50 - 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info - | Notes - |
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Cofilin-2
CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18
Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
Cofilin 1 and Cofilin 2 are actin-binding proteins known also as non-muscle cofilin and muscle cofilin respectively. Together they weigh approximately 19 kDa each. Both are essential in actin filament dynamics catalyzing the disassembly and severing of actin filaments. Cofilin 1 is ubiquitously expressed in various tissues whereas Cofilin 2 is mainly found in muscle tissues and hearts. Their expression patterns and functional properties make them critical in cellular processes involving cytoskeletal reorganization.
These proteins interact with actin to modulate cytoskeletal structure and dynamics. They function by depolymerizing actin filaments an activity that is important in cellular processes like cell migration division and maintenance of cell shape. Cofilin often works as part of a complex with other proteins such as ADF (actin depolymerizing factor) enhancing its ability to modulate actin dynamics. These interactions are integral to cellular scaffolding ensuring proper cell function and adaptation to environmental cues.
These proteins are pivotal in actin dynamics pathways and cellular signaling pathways like the Rho and Rac signaling pathways. The pathways dictate how cells change shape and move in response to signals. ADF and profilin are two significant proteins interacting with cofilin within these pathways. Cofilin essentially modulates actin filament turnover impacting diverse cellular processes and responses. Precise regulation of these pathways is vital for normal cellular behavior.
Cofilin proteins are linked with various conditions including neurodegeneration and cardiomyopathy. Their dysregulation often leads to abnormal actin dynamics contributing to these disorders. For instance in Alzheimer's disease cofilin interacts with proteins like Tau contributing to neurofibrillary tangles. Additionally in cardiomyopathy cofilin dysfunction can negatively affect heart muscle function impacting proteins involved in muscle contraction. Understanding these links provides insights into potential therapeutic targets.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Cofilin 1 + 2 Western blot staining of MDA cell extract using rabbit Anti-Cofilin 1 + 2 antibody
All lanes: Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519) at 1/500 dilution
All lanes: MDA cell extract
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cofilin 1 + 2 with ab131519 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cofilin 1 + 2 with ab131519 at 1/100 dilution.
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