Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Genes 13:2022Epigenetic Regulation Mechanisms of the Cofilin-1 Gene in the Development and Differentiation of Bovine Primary Myoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujia Sun,Yaoyao Ma,Tianqi Zhao,Mingxun Li,Yongjiang Mao,Zhangping YangPubMed 35627108
- The Journal of clinical investigation 129:2807-28232019Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel L Galvan et. al.PubMed 31063459
- Experimental and therapeutic medicine 15:1269-12762018Paclitaxel inhibits breast cancer metastasis via suppression of Aurora kinase-mediated cofilin-1 activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Zhang,Yaoyi Wang,Jun XuePubMed 29434713
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23528
Reactivity data
Select an application| IHC-P | ICC/IF | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/200 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Additional Targets
Cofilin-2
Alternative names
CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18
Recommended products
Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
- Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23528
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cofilin 1 and Cofilin 2 are actin-binding proteins known also as non-muscle cofilin and muscle cofilin respectively. Together they weigh approximately 19 kDa each. Both are essential in actin filament dynamics catalyzing the disassembly and severing of actin filaments. Cofilin 1 is ubiquitously expressed in various tissues whereas Cofilin 2 is mainly found in muscle tissues and hearts. Their expression patterns and functional properties make them critical in cellular processes involving cytoskeletal reorganization.
- Biological function summary
These proteins interact with actin to modulate cytoskeletal structure and dynamics. They function by depolymerizing actin filaments an activity that is important in cellular processes like cell migration division and maintenance of cell shape. Cofilin often works as part of a complex with other proteins such as ADF (actin depolymerizing factor) enhancing its ability to modulate actin dynamics. These interactions are integral to cellular scaffolding ensuring proper cell function and adaptation to environmental cues.
- Pathways
These proteins are pivotal in actin dynamics pathways and cellular signaling pathways like the Rho and Rac signaling pathways. The pathways dictate how cells change shape and move in response to signals. ADF and profilin are two significant proteins interacting with cofilin within these pathways. Cofilin essentially modulates actin filament turnover impacting diverse cellular processes and responses. Precise regulation of these pathways is vital for normal cellular behavior.
- Associated diseases and disorders
Cofilin proteins are linked with various conditions including neurodegeneration and cardiomyopathy. Their dysregulation often leads to abnormal actin dynamics contributing to these disorders. For instance in Alzheimer's disease cofilin interacts with proteins like Tau contributing to neurofibrillary tangles. Additionally in cardiomyopathy cofilin dysfunction can negatively affect heart muscle function impacting proteins involved in muscle contraction. Understanding these links provides insights into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519)
Cofilin 1 + 2 Western blot staining of MDA cell extract using rabbit Anti-Cofilin 1 + 2 antibody
All lanes: Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519) at 1/500 dilution
All lanes: MDA cell extract
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519)
Cofilin 1 + 2 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Cofilin 1 + 2 antibody
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cofilin 1 + 2 with ab131519 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519)
Cofilin 1 + 2 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Cofilin 1 + 2 antibody
Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cofilin 1 + 2 with ab131519 at 1/100 dilution.
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