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Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

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Images

Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (AB131519), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 1 + 2 antibody (AB131519), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin 1 + 2 antibody (AB131519), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23528

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/100 - 1/200
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/100 - 1/200
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/50 - 1/100
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500 - 1/1000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/500 - 1/1000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Target data

Function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

Additional Targets

Cofilin-2

Alternative names

Recommended products

Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogens
  • Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23528
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cofilin 1 and Cofilin 2 are actin-binding proteins known also as non-muscle cofilin and muscle cofilin respectively. Together they weigh approximately 19 kDa each. Both are essential in actin filament dynamics catalyzing the disassembly and severing of actin filaments. Cofilin 1 is ubiquitously expressed in various tissues whereas Cofilin 2 is mainly found in muscle tissues and hearts. Their expression patterns and functional properties make them critical in cellular processes involving cytoskeletal reorganization.

Biological function summary

These proteins interact with actin to modulate cytoskeletal structure and dynamics. They function by depolymerizing actin filaments an activity that is important in cellular processes like cell migration division and maintenance of cell shape. Cofilin often works as part of a complex with other proteins such as ADF (actin depolymerizing factor) enhancing its ability to modulate actin dynamics. These interactions are integral to cellular scaffolding ensuring proper cell function and adaptation to environmental cues.

Pathways

These proteins are pivotal in actin dynamics pathways and cellular signaling pathways like the Rho and Rac signaling pathways. The pathways dictate how cells change shape and move in response to signals. ADF and profilin are two significant proteins interacting with cofilin within these pathways. Cofilin essentially modulates actin filament turnover impacting diverse cellular processes and responses. Precise regulation of these pathways is vital for normal cellular behavior.

Associated diseases and disorders

Cofilin proteins are linked with various conditions including neurodegeneration and cardiomyopathy. Their dysregulation often leads to abnormal actin dynamics contributing to these disorders. For instance in Alzheimer's disease cofilin interacts with proteins like Tau contributing to neurofibrillary tangles. Additionally in cardiomyopathy cofilin dysfunction can negatively affect heart muscle function impacting proteins involved in muscle contraction. Understanding these links provides insights into potential therapeutic targets.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Terms & Conditions.

3 product images

  • Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519), expandable thumbnail

    Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519)

    Cofilin 1 + 2 Western blot staining of MDA cell extract using rabbit Anti-Cofilin 1 + 2 antibody

    All lanes: Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519) at 1/500 dilution

    All lanes: MDA cell extract

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 1 + 2 antibody (ab131519), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 1 + 2 antibody (ab131519)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cofilin 1 + 2 with ab131519 at 1/50 dilution. Right panel was preincubated with blocking peptide.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin 1 + 2 antibody (ab131519), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cofilin 1 + 2 antibody (ab131519)

    Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cofilin 1 + 2 with ab131519 at 1/100 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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