AB131519

Anti-Cofilin 1 + 2 antibody - Loading Control

Name: Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519) | Abcam
Brand: Abcam Limited
SKU: AB131519
Price: 520 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 50-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin-2, CFL2

3 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 1 + 2 antibody - Loading Control (AB131519)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Cofilin 1 + 2 with ab131519 at 1/50 dilution. Right panel was preincubated with blocking peptide.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin 1 + 2 antibody - Loading Control (AB131519)

Immunofluorescent analysis of methanol-fixed HeLa cells labelling Cofilin 1 + 2 with ab131519 at 1/100 dilution.

Unknown

Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (AB131519)

All lanes:

Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (ab131519) at 1/500 dilution

All lanes:

MDA cell extract

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human CFL2 aa 50-100 conjugated to Keyhole Limpet Haemocyanin. Database link Q9Y281

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/200", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin 1 and Cofilin 2 are actin-binding proteins known also as non-muscle cofilin and muscle cofilin respectively. Together they weigh approximately 19 kDa each. Both are essential in actin filament dynamics catalyzing the disassembly and severing of actin filaments. Cofilin 1 is ubiquitously expressed in various tissues whereas Cofilin 2 is mainly found in muscle tissues and hearts. Their expression patterns and functional properties make them critical in cellular processes involving cytoskeletal reorganization.

Biological function summary

These proteins interact with actin to modulate cytoskeletal structure and dynamics. They function by depolymerizing actin filaments an activity that is important in cellular processes like cell migration division and maintenance of cell shape. Cofilin often works as part of a complex with other proteins such as ADF (actin depolymerizing factor) enhancing its ability to modulate actin dynamics. These interactions are integral to cellular scaffolding ensuring proper cell function and adaptation to environmental cues.

Pathways

These proteins are pivotal in actin dynamics pathways and cellular signaling pathways like the Rho and Rac signaling pathways. The pathways dictate how cells change shape and move in response to signals. ADF and profilin are two significant proteins interacting with cofilin within these pathways. Cofilin essentially modulates actin filament turnover impacting diverse cellular processes and responses. Precise regulation of these pathways is vital for normal cellular behavior.

Cofilin proteins are linked with various conditions including neurodegeneration and cardiomyopathy. Their dysregulation often leads to abnormal actin dynamics contributing to these disorders. For instance in Alzheimer's disease cofilin interacts with proteins like Tau contributing to neurofibrillary tangles. Additionally in cardiomyopathy cofilin dysfunction can negatively affect heart muscle function impacting proteins involved in muscle contraction. Understanding these links provides insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed : 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed : 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed : 21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

See full target information CFL1

Additional targets

Cofilin-2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Genes 13: PubMed35627108

2022

Epigenetic Regulation Mechanisms of the Cofilin-1 Gene in the Development and Differentiation of Bovine Primary Myoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Sun,Yaoyao Ma,Tianqi Zhao,Mingxun Li,Yongjiang Mao,Zhangping Yang

The Journal of clinical investigation 129:2807-2823 PubMed31063459

2019

Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel L Galvan,Jianyin Long,Nathanael Green,Benny H Chang,Jamie S Lin,Paul Schumacker,Luan D Truong,Paul Overbeek,Farhad R Danesh

Experimental and therapeutic medicine 15:1269-1276 PubMed29434713

2018

Paclitaxel inhibits breast cancer metastasis via suppression of Aurora kinase-mediated cofilin-1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Yaoyi Wang,Jun Xue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cofilin 1 + 2 antibody - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 1 + 2 antibody - Loading Control (AB131519)

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin 1 + 2 antibody - Loading Control (AB131519)

Western blot - Anti-Cofilin 1 + 2 antibody - Loading Control (AB131519)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

Product promise