AB300128

Anti-Cofilin 2 antibody [EPR24519-7]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Cofilin 2 antibody. Suitable for WB, IHC-P, Flow Cyt (Intra), IP and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Cofilin-2, CFL2

14 Images

Flow Cytometry (Intracellular) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling Cofilin 2 with ab300128 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded human skeletal muscl tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Positive staining on human skeletal muscle (PMID : 24610938).

The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection) was used.

Positive staining on human cardiac muscle.

The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Cofilin 2 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10µg with ab300128 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300128 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 10 µg

Lane 2:

ab300128 at 1/30 IP in Raji whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300128 in Raji whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 18 kDa

false

Exposure time: 23.684s

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Positive staining on rat cardiac muscle.

The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Cofilin 2 with ab300128 at 1/2000 (0.232 µg/ml) followed by a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

Positive staining on mouse cardiac muscle (PMID : 11809832).

The section was incubated with ab300128 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : PBS was used instead of primary antibody (ab300128) followed by ready to use secondary antibody LeicaDS9800 (BOND™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (mouse myoblasts) cells labelling Cofilin 2 with ab300128 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Lab

Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Cofilin 2 was immunoprecipitated from 0.35 mg Mouse heart tissue lysate 10µg with ab300128 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300128 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

ab300128 at 1/30 IP in mouse heart tissue lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300128 in Mouse heart tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 18 kDa

false

Exposure time: 5.5s

Lab

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining.

All lanes:

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1:

Hela (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

Hela transfected with siRNA specifically targeting Cofilin-2, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 147s

Lab

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Cofilin 1.

Both recombinant proteins were expressed from E.coli expression systems.

All lanes:

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1:

His-tagged human Cofilin 2 recombinant protein at 10 ng

Lane 2:

His-tagged human Cofilin 1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 26s

Lab

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Observed band size: 18 kDa

false

Exposure time: 37s

Lab

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 37s

Lab

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

C2C12 (mouse myoblasts) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 26s

Lab

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (ab300128) at 1/1000 dilution

Lane 1:

Rat heart tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 18 kDa

false

Exposure time: 26s

Carrier free

Anti-Cofilin 2 antibody [EPR24519-7] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24519-7

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

WB, Flow Cyt (Intra), IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin 2 also referred to as CFL2 is an actin-binding protein that plays a significant role in actin dynamics by disassembling actin filaments. It has a molecular mass of approximately 19 kDa. This protein is expressed predominantly in muscle tissues including skeletal and cardiac muscle. Cofilin 2 is integral in the maintenance and reorganization of the actin cytoskeleton facilitating cellular movement and shape changes.

Biological function summary

Cofilin 2 is involved in processes including muscle contraction and cytoskeletal organization. It participates in the depolymerization of actin filaments thereby accelerating filament turnover and recycling. It does not function in isolation but interacts with several proteins in the cytoskeleton forming a complex interplay that modulates actin filament dynamics. Its activity is regulated by phosphorylation with specific kinases and phosphatases modulating its function based on cellular signals.

Pathways

Cofilin 2 is an important element of the actin reorganization pathway and is involved in the Rho family GTPase signaling pathway. It works closely with proteins like LIM kinase which phosphorylates cofilin and inhibits its activity and slingshot phosphatase which dephosphorylates and activates it. This regulation ensures proper actin filament dynamics which is essential for processes like cell division migration and muscle function.

Cofilin 2 mutations have been associated with nemaline myopathy a muscle disorder characterized by muscle weakness. This disorder often involves abnormalities in the neuromuscular junction where cofilin 2 interacts with actin and related proteins like tropomyosin affecting muscle contraction. Additionally altered cofilin 2 expression links to cardiac complications due to its role in cardiac muscle maintenance and actin filament dynamics.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed : 19752190). It has the ability to bind G- and F-actin in a 1 : 1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).

See full target information CFL2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:1446-1459 PubMed40437054

2025

Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development.

Applications

Unspecified application

Species

Unspecified reactive species

Ya'el Courtney,Joshua P Head,Neil Dani,Olga V Chechneva,Frederick B Shipley,Yong Zhang,Michael J Holtzman,Cameron Sadegh,Towia A Libermann,Maria K Lehtinen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cofilin 2 antibody [EPR24519-7]

Flow Cytometry (Intracellular) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Flow Cytometry (Intracellular) - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Immunoprecipitation - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Western blot - Anti-Cofilin 2 antibody [EPR24519-7] (AB300128)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise