AB54532

Anti-Cofilin antibody [1A1] - Loading Control

Name: Anti-Cofilin antibody [1A1] Mouse monoclonal (ab54532) | Abcam
Brand: Abcam Limited
SKU: AB54532
Price: 400 USD
Availability: InStock
Rating: 4 (6 reviews)

Lab Essentials
What is this?

(6 Reviews)

(37 Publications)

Mouse Monoclonal Cofilin-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 37 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CFL1.

View Alternative Names

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18

5 Images

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Cofilin with ab54532 at 1.5 μg/ml in immunohistochemical analysis.

This image was generated using the ascites version of the product.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Cofilin (green) using ab54532 at 10 μg/ml in ICC/IF.

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

This image was generated using the ascites version of the product

All lanes:

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (ab54532) at 1 µg

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 25 µg

Predicted band size: 18 kDa

false

Unknown

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (ab54532) at 1 µg/mL

All lanes:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Predicted band size: 18 kDa

false

Unknown

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (ab54532) at 1 µg/mL

All lanes:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Predicted band size: 18 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1A1

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CFL1.

P23528

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.

Biological function summary

Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.

Pathways

Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.

There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed : 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed : 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed : 21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

See full target information CFL1

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants (Basel, Switzerland) 14: PubMed40563335

2025

CSE-Induced ER-Mitochondria Crosstalk Promotes Oxidative Stress and Impairs Bronchial Contractile Response.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge Rodríguez-Pérez,Rosa Andreu-Martínez,Leila Pérez-Sánchez,Ana Hernández-García,Cecilia Muñoz-Calleja,Ángel Cogolludo,María J Calzada

Cell death & disease 15:264 PubMed38615035

2024

α-Synuclein triggers cofilin pathology and dendritic spine impairment via a PrP-CCR5 dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Marina I Oliveira da Silva,Miguel Santejo,Isaac W Babcock,Ana Magalhães,Laurie S Minamide,Seok-Joon Won,Erika Castillo,Ellen Gerhardt,Christiane Fahlbusch,Raymond A Swanson,Tiago F Outeiro,Ricardo Taipa,Michael Ruff,James R Bamburg,Márcia A Liz

Genes, brain, and behavior 22:e12863 PubMed37575018

2023

Impaired erythropoietin-producing hepatocellular B receptors signaling in the prefrontal cortex and hippocampus following maternal immune activation in male rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqian Shao,Yaqi Cai,Tengfei Chen,Keke Hao,Binbin Luo,Xiujuan Wang,Weiyun Guo,Xi Su,Luxian Lv,Yongfeng Yang,Wenqiang Li

Frontiers in molecular neuroscience 15:1013182 PubMed36277486

2022

Plasticity of the hypocretinergic/orexinergic system after a chronic treatment with suvorexant in rats. Role of the hypocretinergic/orexinergic receptor 1 as an autoreceptor.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Carrera-Cañas,Isabel de Andrés,Marta Callejo,Miguel Garzón

The EMBO journal 41:e110192 PubMed36149731

2022

Stimulus-specific remodeling of the neuronal transcriptome through nuclear intron-retaining transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Maxime Mazille,Katarzyna Buczak,Peter Scheiffele,Oriane Mauger

Theranostics 12:5034-5050 PubMed35836807

2022

SGLT2 inhibitor dapagliflozin reduces endothelial dysfunction and microvascular damage during cardiac ischemia/reperfusion injury through normalizing the XO-SERCA2-CaMKII-coffilin pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Rongjun Zou,Wanting Shi,Na Zhou,Shaoxian Chen,Hao Zhou,Xinxin Chen,Yueheng Wu

Frontiers in medicine 9:861371 PubMed35492354

2022

Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lujia Feng,Haichun Li,Yong Du,Ting Zhang,Yingting Zhu,Zhidong Li,Ling Zhao,Xing Wang,Gongpei Wang,Linbin Zhou,Zhaorong Jiang,Zheng Liu,Zhancong Ou,Yuwen Wen,Yehong Zhuo

International journal of molecular sciences 23: PubMed35269714

2022

Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Krishnatej Nishtala,Trailokyanath Panigrahi,Rohit Shetty,Dhanananajay Kumar,Pooja Khamar,Rajiv R Mohan,Vrushali Deshpande,Arkasubhra Ghosh

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:2190-2204 PubMed35135857

2022

Inositol Polyphosphate-5-Phosphatase K () Enhances Sprouting of Corticospinal Tract Axons after CNS Trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Sierra D Kauer,Kathryn L Fink,Elizabeth H F Li,Brian P Evans,Noa Golan,William B J Cafferty

The Journal of cell biology 221: PubMed35024764

2022

Eukaryotic initiation factor 6 regulates mechanical responses in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adam N Keen,Luke A Payne,Vedanta Mehta,Alistair Rice,Lisa J Simpson,Kar Lai Pang,Armando Del Rio Hernandez,John S Reader,Ellie Tzima

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cofilin antibody [1A1] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Western blot - Anti-Cofilin antibody [1A1] - Loading Control (AB54532)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (37)

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