AB11062

Anti-Cofilin antibody

Name: Anti-Cofilin antibody (ab11062) | Abcam
Brand: Abcam Limited
SKU: AB11062
Price: 400 USD
Availability: InStock
Rating: 4 (3 reviews)

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What is this?

(3 Reviews)

(32 Publications)

Rabbit Polyclonal Cofilin-1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 32 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (AB11062)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (AB11062)

ICC/IF image of ab11062 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11062, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Synthetic Peptide within Human CFL1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P23528

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

Whole antiserum is fractionated and further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.

Biological function summary

Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.

Pathways

Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.

There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed : 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed : 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed : 21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

See full target information CFL1

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Publications (32)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112845 PubMed37480564

2023

PCDH12 loss results in premature neuronal differentiation and impeded migration in a cortical organoid model.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Rakotomamonjy,Lauren Rylaarsdam,Lucas Fares-Taie,Sean McDermott,Devin Davies,George Yang,Fikayo Fagbemi,Maya Epstein,Martín Fairbanks-Santana,Jean-Michel Rozet,Alicia Guemez-Gamboa

Nature communications 14:700 PubMed36755029

2023

TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Sujin Kang,Jaekyung Kim,Areum Park,Minsoo Koh,Wonji Shin,Gayoung Park,Taeyun A Lee,Hyung Jin Kim,Heonjong Han,Yongbo Kim,Myung Kyung Choi,Jae Hyung Park,Eunhye Lee,Hyun-Soo Cho,Hyun Woo Park,Jae Hee Cheon,Sungwook Lee,Boyoun Park

Nature communications 13:6037 PubMed36229429

2022

INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Calabrese,Steven L Jones,Yoko Shiraishi-Yamaguchi,Michael Lingelbach,Uri Manor,Tatyana M Svitkina,Henry N Higgs,Andy Y Shih,Shelley Halpain

Nature biomedical engineering 5:1485-1499 PubMed33875841

2021

Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Cierra J Walker,Claudia Crocini,Daniel Ramirez,Anouk R Killaars,Joseph C Grim,Brian A Aguado,Kyle Clark,Mary A Allen,Robin D Dowell,Leslie A Leinwand,Kristi S Anseth

Arteriosclerosis, thrombosis, and vascular biology 40:175-188 PubMed31694393

2019

S-Nitrosylation of Plastin-3 Exacerbates Thoracic Aortic Dissection Formation via Endothelial Barrier Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Lihong Pan,Zhe Lin,Xin Tang,Jiaxin Tian,Qiao Zheng,Jin Jing,Liping Xie,Hongshan Chen,Qiulun Lu,Hong Wang,Qingguo Li,Yi Han,Yong Ji

Virologica Sinica 35:143-155 PubMed31429011

2019

Proteomic Profiling of Purified Rabies Virus Particles.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Yuyang Wang,Ye Feng,Zhongzhong Tu,Zhiyong Lou,Changchun Tu

Cerebral cortex (New York, N.Y. : 1991) 29:5204-5216 PubMed30953439

2019

FMRP Modulates Activity-Dependent Spine Plasticity by Binding Cofilin1 mRNA and Regulating Localization and Local Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Feuge,Franziska Scharkowski,Kristin Michaelsen-Preusse,Martin Korte

Cellular signalling 58:65-78 PubMed30844428

2019

Fibulin-4 deficiency differentially affects cytoskeleton structure and dynamics as well as TGFβ signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Joyce Burger,Nicole van Vliet,Paula van Heijningen,Heena Kumra,Gert-Jan Kremers,Maria Alves,Gert van Cappellen,Hiromi Yanagisawa,Dieter P Reinhardt,Roland Kanaar,Ingrid van der Pluijm,Jeroen Essers

Nature physics 15:393-402 PubMed30984281

2019

Large-scale curvature sensing by directional actin flow drives cellular migration mode switching.

Applications

Unspecified application

Species

Unspecified reactive species

Tianchi Chen,Andrew Callan-Jones,Eduard Fedorov,Andrea Ravasio,Agustí Brugués,Hui Ting Ong,Yusuke Toyama,Boon Chuan Low,Xavier Trepat,Tom Shemesh,Raphaël Voituriez,Benoît Ladoux

Oncogene 37:3778-3789 PubMed29643476

2018

Inhibition of ovarian tumor cell invasiveness by targeting SYK in the tyrosine kinase signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yu,Yohan Suryo Rahmanto,Meng-Horng Lee,Pei-Hsun Wu,Jude M Phillip,Chuan-Hsiang Huang,Michele I Vitolo,Stephanie Gaillard,Stuart S Martin,Denis Wirtz,Ie-Ming Shih,Tian-Li Wang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cofilin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (AB11062)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (32)

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