Rabbit Polyclonal Cofilin-1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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- Nature communications 14:7002023TRIM40 is a pathogenic driver of inflammatory bowel disease subverting intestinal barrier integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSujin Kang,Jaekyung Kim,Areum Park,Minsoo Koh,Wonji Shin,Gayoung Park,Taeyun A Lee,Hyung Jin Kim,Heonjong Han,Yongbo Kim,Myung Kyung Choi,Jae Hyung Park,Eunhye Lee,Hyun-Soo Cho,Hyun Woo Park,Jae Hee Cheon,Sungwook Lee,Boyoun ParkPubMed 36755029
- Nature communications 13:60372022INF2-mediated actin filament reorganization confers intrinsic resilience to neuronal ischemic injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Calabrese,Steven L Jones,Yoko Shiraishi-Yamaguchi,Michael Lingelbach,Uri Manor,Tatyana M Svitkina,Henry N Higgs,Andy Y Shih,Shelley HalpainPubMed 36229429
- Nature biomedical engineering 5:1485-14992021Nuclear mechanosensing drives chromatin remodelling in persistently activated fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCierra J Walker,Claudia Crocini,Daniel Ramirez,Anouk R Killaars,Joseph C Grim,Brian A Aguado,Kyle Clark,Mary A Allen,Robin D Dowell,Leslie A Leinwand,Kristi S AnsethPubMed 33875841
- Virologica Sinica 35:143-1552019Proteomic Profiling of Purified Rabies Virus Particles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhang,Yuyang Wang,Ye Feng,Zhongzhong Tu,Zhiyong Lou,Changchun TuPubMed 31429011
- Cerebral cortex (New York, N.Y. : 1991) 29:5204-52162019FMRP Modulates Activity-Dependent Spine Plasticity by Binding Cofilin1 mRNA and Regulating Localization and Local Translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonas Feuge,Franziska Scharkowski,Kristin Michaelsen-Preusse,Martin KortePubMed 30953439
- Cellular signalling 58:65-782019Fibulin-4 deficiency differentially affects cytoskeleton structure and dynamics as well as TGFβ signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoyce Burger et. al.PubMed 30844428
- Nature physics 15:393-4022019Large-scale curvature sensing by directional actin flow drives cellular migration mode switching.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianchi Chen et. al.PubMed 30984281
- Oncogene 37:3778-37892018Inhibition of ovarian tumor cell invasiveness by targeting SYK in the tyrosine kinase signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Yu et. al.PubMed 29643476
- Scientific reports 7:89242017PDGF-BB enhances collagen gel contraction through a PI3K-PLCγ-PKC-cofilin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVahid Reyhani et. al.PubMed 28827622
- Journal of inflammation (London, England) 14:102017WIF1 prevents Wnt5A mediated LIMK/CFL phosphorylation and adherens junction disruption in human vascular endothelial cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CFL1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23528
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Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
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Target data
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Alternative names
CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18
Recommended products
Rabbit Polyclonal Cofilin-1 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 29 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CFL1 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P23528
- Purity
- IgG fraction
- Concentration
- Purification notes
Whole antiserum is fractionated and further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.
- Biological function summary
Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.
- Pathways
Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.
- Associated diseases and disorders
There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.
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1 product image
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab11062)
ICC/IF image of ab11062 stained human HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11062, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
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