Rabbit Recombinant Monoclonal Cofilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications.
Images
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img72017.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)")
![Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--immunocytochemistry-immunofluorescence-img108586.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)")
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img57762.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)")
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img61277.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)")
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img124685.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)")
Publications
- Human cell 36:1928-19372023Cofilin-1 induces acute kidney injury via the promotion of endoplasmic reticulum stress-mediated ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSihao Lin,Jie Wang,Bin Cao,Yang Huang,Xujun Sheng,Yingjian ZhuPubMed 37548903
- Molecular cancer therapeutics 21:903-9132022Comprehensive Surfaceome Profiling to Identify and Validate Novel Cell-Surface Targets in Osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifei Wang,Xiangjun Tian,Wendong Zhang,Zhongting Zhang,Rossana Lazcano,Pooja Hingorani,Michael E Roth,Jonathan D Gill,Douglas J Harrison,Zhaohui Xu,Sylvester Jusu,Sankaranarayanan Kannan,Jing Wang,Alexander J Lazar,Eric J Earley,Stephen W Erickson,Tara Gelb,Philip Huxley,Johanna Lahdenranta,Gemma Mudd,Raushan T Kurmasheva,Peter J Houghton,Malcolm A Smith,Edward A Kolb,Richard GorlickPubMed 35312779
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Expected |
| Rat | Not recommended | Tested | Expected |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
9 products for Alternative Product
1 products for Alternative Version
Target data
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Alternative names
Cofilin-1, 18 kDa phosphoprotein, p18, CFL, CFL1
Recommended products
Rabbit Recombinant Monoclonal Cofilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications.
Alternative names
Cofilin-1, 18 kDa phosphoprotein, p18, CFL, CFL1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR6375
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img72017.jpg "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)")
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution
Lane 1: NIH/3T3 cell lysate at 20 µg
Lane 2: C6 cell lysate at 20 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--immunocytochemistry-immunofluorescence-img108586.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)")
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
The nuclear counterstain is DAPI (blue).
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img57762.jpg "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)")
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution
All lanes: HEK293 cell lysate at 10 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img61277.jpg "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)")
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/5000 dilution
All lanes: Caco-2 cell lysate at 10 µg
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa
![Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979), expandable thumbnail](https://content.abcam.com/products/images/cofilin-antibody-epr6375-loading-control-ab124979--western-blot-img124685.jpg "Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)")
Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: Caco-2 cell lysate at 10 µg
Predicted band size: 18 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com