AB124979

Anti-Cofilin antibody [EPR6375] - Loading Control

RabMAb
Recombinant
Lab Essentials
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(12 Publications)

Rabbit Recombinant Monoclonal Cofilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 12 publications.

View Alternative Names

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18

6 Images

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

The nuclear counterstain is DAPI (blue).

Unknown

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

All lanes:

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Predicted band size: 18 kDa

false

Unknown

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution

All lanes:

HEK293 cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 18 kDa

Observed band size: 19 kDa

false

Unknown

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/5000 dilution

All lanes:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 18 kDa

Observed band size: 19 kDa

false

Unknown

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979) at 1/1000 dilution

Lane 1:

NIH/3T3 cell lysate at 20 µg

Lane 2:

C6 cell lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 18 kDa

Observed band size: 19 kDa

false

CiteAb

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Cofilin western blot using anti-Cofilin antibody [EPR6375] - Loading Control ab124979. Publication image and figure legend from Yang, W., Chen, Z., et al., 2020, Cell Prolif, PubMed 31943490.

ab124979 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab124979 please see the product overview.

Genetically modified ADSCs prevented fibrosis of cavernosal tissues after BCNI by downregulating the p-LIMK2/p-cofilin pathway. A, Representative images of western blots for phosphorylated LIMK2 (p-LIMK2), LIMK2, phosphorylated cofilin (p-cofilin) and cofilin in cavernosal tissues from each group. B,C, Quantitative analysis using the ratio of phosphorylated protein to total protein. Data are depicted as the mean ± SD from n = 6 animals per group (**p < .01 and *p < .05); one-way ANOVA followed by the S-N-K test

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Carrier free

Anti-Cofilin antibody [EPR6375] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6375

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.

Biological function summary

Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.

Pathways

Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.

There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed : 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed : 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed : 21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

See full target information CFL1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal and liver diseases : JGLD 34:30-39 PubMed40153818

2025

Programmed Cell Death-ligand 1 as Biomarker of Poor Prognosis in Patients with Gastrointestinal Stromal Tumour.

Applications

Unspecified application

Species

Unspecified reactive species

Duolikun Yasheng,Abuduwaili Aierken,Yiliang Li,Aikebaier Aili,Kelimu Abudureyimu

Human cell 36:1928-1937 PubMed37548903

2023

Cofilin-1 induces acute kidney injury via the promotion of endoplasmic reticulum stress-mediated ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sihao Lin,Jie Wang,Bin Cao,Yang Huang,Xujun Sheng,Yingjian Zhu

American journal of translational research 14:6243-6255 PubMed36247237

2022

Methionine aminopeptidase 2 as a potential target in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Eliana Steinberg,Rawnaq Esa,Ouri Schwob,Tal Stern,Natalie Orehov,Gideon Zamir,Ayala Hubert,Dipak Panigrahy,Ofra Benny

Molecular cancer therapeutics 21:903-913 PubMed35312779

2022

Comprehensive Surfaceome Profiling to Identify and Validate Novel Cell-Surface Targets in Osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Wang,Xiangjun Tian,Wendong Zhang,Zhongting Zhang,Rossana Lazcano,Pooja Hingorani,Michael E Roth,Jonathan D Gill,Douglas J Harrison,Zhaohui Xu,Sylvester Jusu,Sankaranarayanan Kannan,Jing Wang,Alexander J Lazar,Eric J Earley,Stephen W Erickson,Tara Gelb,Philip Huxley,Johanna Lahdenranta,Gemma Mudd,Raushan T Kurmasheva,Peter J Houghton,Malcolm A Smith,Edward A Kolb,Richard Gorlick

Frontiers in cell and developmental biology 9:644716 PubMed33791303

2021

Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhang,Zongtao Chai,Siyuan Kong,Jiling Feng,Man Wu,Jiaqi Tan,Man Yuan,Gan Chen,Zhuo Li,Hua Zhou,Shuqun Cheng,Hongxi Xu

Cancer cell international 20:589 PubMed33298070

2020

ETS1-activated SNHG10 exerts oncogenic functions in glioma via targeting miR-532-3p/FBXL19 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lide Jin,Shengquan Huang,Congjin Guan,Shun Chang

Cell death & disease 11:981 PubMed33191397

2020

ZEB1-activated LINC01123 accelerates the malignancy in lung adenocarcinoma through NOTCH signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Zhang,Yaguang Han,Yi Zheng,Yan Zhang,Xin Zhao,Zhenlin Gao,Xinyan Liu

Cell proliferation 53:e12756 PubMed31943490

2020

Co-overexpression of VEGF and GDNF in adipose-derived stem cells optimizes therapeutic effect in neurogenic erectile dysfunction model.

Applications

Unspecified application

Species

Unspecified reactive species

Wende Yang,Zehong Chen,Xiaolei Ma,Xi Ouyang,Jiafeng Fang,Hongbo Wei

Cancer management and research 11:4781-4796 PubMed31213900

2019

Eupatilin inhibits glioma proliferation, migration, and invasion by arresting cell cycle at G1/S phase and disrupting the cytoskeletal structure.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Fei,Ji Wang,Chen Chen,Boyun Ding,Xiaojun Fu,Wenjing Chen,Chongwu Wang,Ruxiang Xu

Scientific reports 8:921 PubMed29343717

2018

The role of NFκB in spheroid formation of human breast cancer cells cultured on the Random Positioning Machine.

Applications

Species

Human

Sascha Kopp,Jayashree Sahana,Tawhidul Islam,Asbjørn Graver Petersen,Johann Bauer,Thomas J Corydon,Herbert Schulz,Kathrin Saar,Norbert Huebner,Lasse Slumstrup,Stefan Riwaldt,Markus Wehland,Manfred Infanger,Ronald Luetzenberg,Daniela Grimm

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cofilin antibody [EPR6375] - Loading Control

Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise