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Rabbit Recombinant Monoclonal Cofilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications.

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Images

Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (AB124979), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (AB124979), expandable thumbnail
  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (AB124979), expandable thumbnail
  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (AB124979), expandable thumbnail
  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (AB124979), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Expected
Rat
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/200
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

Alternative names

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18

Recommended products

Rabbit Recombinant Monoclonal Cofilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6375
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.

Biological function summary

Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.

Pathways

Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.

Associated diseases and disorders

There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979), expandable thumbnail

    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979)

    Cofilin Western blot staining using rabbit Anti-Cofilin antibody

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/1000 dilution

    Lane 1: NIH/3T3 cell lysate at 20 µg

    Lane 2: C6 cell lysate at 20 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa

    Observed band size: 19 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody [EPR6375] - Loading Control (ab124979)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    The nuclear counterstain is DAPI (blue).

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979), expandable thumbnail

    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979)

    Cofilin Western blot staining of HEK293 cell lysate using rabbit Anti-Cofilin antibody

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/1000 dilution

    All lanes: HEK293 cell lysate at 10 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa

    Observed band size: 19 kDa

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979), expandable thumbnail

    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979)

    Cofilin Western blot staining of Caco-2 cell lysate using rabbit Anti-Cofilin antibody

    Blocking Buffer: 5% NFDM/TBST

    Dilution Buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/5000 dilution

    All lanes: Caco-2 cell lysate at 10 µg

    Secondary

    All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 18 kDa

    Observed band size: 19 kDa

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979), expandable thumbnail

    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979)

    Cofilin Western blot staining using rabbit Anti-Cofilin antibody

    All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: Caco-2 cell lysate at 10 µg

    Predicted band size: 18 kDa

  • Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979)

    Cofilin Western blot staining using rabbit Anti-Cofilin antibody

    Cofilin western blot using anti-Cofilin antibody [EPR6375] - Loading Control ab124979. Publication image and figure legend from Yang, W., Chen, Z., et al., 2020, Cell Prolif, PubMed 31943490.


    ab124979 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab124979 please see the product overview.

    Genetically modified ADSCs prevented fibrosis of cavernosal tissues after BCNI by downregulating the p‐LIMK2/p‐cofilin pathway. A, Representative images of western blots for phosphorylated LIMK2 (p‐LIMK2), LIMK2, phosphorylated cofilin (p‐cofilin) and cofilin in cavernosal tissues from each group. B,C, Quantitative analysis using the ratio of phosphorylated protein to total protein. Data are depicted as the mean ± SD from n = 6 animals per group (**P < .01 and *P < .05); one‐way ANOVA followed by the S‐N‐K test

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Product protocols

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