Rabbit Recombinant Monoclonal Cofilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Not recommended | Tested | Tested |
Mouse | Not recommended | Tested | Expected |
Rat | Not recommended | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18
Rabbit Recombinant Monoclonal Cofilin-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications.
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.
Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.
Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.
There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Cofilin Western blot staining using rabbit Anti-Cofilin antibody
Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/1000 dilution
Lane 1: NIH/3T3 cell lysate at 20 µg
Lane 2: C6 cell lysate at 20 µg
All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lines labeling Cofilin with ab124979 at 1/500 dilution,followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
The nuclear counterstain is DAPI (blue).
Cofilin Western blot staining of HEK293 cell lysate using rabbit Anti-Cofilin antibody
Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/1000 dilution
All lanes: HEK293 cell lysate at 10 µg
All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa
Cofilin Western blot staining of Caco-2 cell lysate using rabbit Anti-Cofilin antibody
Blocking Buffer: 5% NFDM/TBST
Dilution Buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/5000 dilution
All lanes: Caco-2 cell lysate at 10 µg
All lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 19 kDa
Cofilin Western blot staining using rabbit Anti-Cofilin antibody
All lanes: Western blot - Anti-Cofilin antibody [EPR6375] - Loading Control - Loading Control (ab124979) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: Caco-2 cell lysate at 10 µg
Predicted band size: 18 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Cofilin Western blot staining using rabbit Anti-Cofilin antibody
Cofilin western blot using anti-Cofilin antibody [EPR6375] - Loading Control ab124979. Publication image and figure legend from Yang, W., Chen, Z., et al., 2020, Cell Prolif, PubMed 31943490.
ab124979 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab124979 please see the product overview.
Genetically modified ADSCs prevented fibrosis of cavernosal tissues after BCNI by downregulating the p‐LIMK2/p‐cofilin pathway. A, Representative images of western blots for phosphorylated LIMK2 (p‐LIMK2), LIMK2, phosphorylated cofilin (p‐cofilin) and cofilin in cavernosal tissues from each group. B,C, Quantitative analysis using the ratio of phosphorylated protein to total protein. Data are depicted as the mean ± SD from n = 6 animals per group (**P < .01 and *P < .05); one‐way ANOVA followed by the S‐N‐K test
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