AB29038

Anti-Cofilin antibody - Loading Control

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(3 Publications)

Rabbit Polyclonal Cofilin-1 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFL1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18

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Western blot - Anti-Cofilin antibody - Loading Control (AB29038)

Western blot of jurkat cells expressing cofilin 1. Blots were treated with (lanes 2, 3, 5 & 6) or without (lanes 1 & 4) lambda phosphatase for 1 hr at 37 degrees C. In lanes 3 & 6, phosphatase treatments were performed in the presence of phospho-cofilin 1 (Ser-3) peptide. Blots were probed with anti-phospho-Cofilin 1 (Ser-3) (lanes 1-3;) or anti-Cofilin 1 (lanes 4-6; ab29038). Phosphatase treatment removes Ser-3 phosphorylation of Cofilin, but this phosphatase activity is reduced in the presence of peptide.

All lanes:

Western blot - Anti-Cofilin antibody - Loading Control (ab29038)

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CFL1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P23528

Specificity

ab29038 recognises Cofilin 1. The immunogen sequence has only 3 amino acid differences from human Cofilin 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.

Biological function summary

Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.

Pathways

Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.

There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed : 11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed : 15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed : 21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed : 23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).

See full target information CFL1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 13:4414-20 PubMed27052944

2016

Inhibition of the Rac1-WAVE2-Arp2/3 signaling pathway promotes radiosensitivity via downregulation of cofilin-1 in U251 human glioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhou,Chen-Han Wang,Hua Yan,Rui Zhang,Jin-Bing Zhao,Chun-Fa Qian,Hong Xiao,Hong-Yi Liu

Journal of proteomics 94:327-36 PubMed24129212

2013

Investigation of adipocyte proteome during the differentiation of brown preadipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Abu Hena Mostafa Kamal,Won Kon Kim,Kun Cho,Anna Park,Jeong-Ki Min,Baek Soo Han,Sung Goo Park,Sang Chul Lee,Kwang-Hee Bae

Molecular & cellular proteomics : MCP 8:670-80 PubMed19054759

2008

Global analysis of protein damage by the lipid electrophile 4-hydroxy-2-nonenal.

Applications

Species

Human

Simona G Codreanu,Bing Zhang,Scott M Sobecki,Dean D Billheimer,Daniel C Liebler

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Cofilin antibody - Loading Control

Western blot - Anti-Cofilin antibody - Loading Control (AB29038)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise