Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 80 publications.
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- Breast cancer research : BCR 25:1192023MiR-4649-5p acts as a tumor-suppressive microRNA in triple negative breast cancer by direct interaction with PIP5K1C, thereby potentiating growth-inhibitory effects of the AKT inhibitor capivasertib.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatharina Jonas,Felix Prinz,Manuela Ferracin,Katarina Krajina,Barbara Pasculli,Alexander Deutsch,Tobias Madl,Beate Rinner,Ondrej Slaby,Christiane Klec,Martin PichlerPubMed 37803350
- Oncogenesis 12:252023miR-137-LAPTM4B regulates cytoskeleton organization and cancer metastasis via the RhoA-LIMK-Cofilin pathway in osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuyu Yan,Dan Liu,Junjie Wang,Minxia Liu,Hongjuan Guo,Jing Bai,Shuo Yang,Jun Chang,Zhihong Yao,Zuozhang Yang,Tomas Blom,Kecheng ZhouPubMed 37147294
- eLife 12:2023High-content synaptic phenotyping in human cellular models reveals a role for BET proteins in synapse assembly.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartin H Berryer,Gizem Rizki,Anna Nathanson,Jenny A Klein,Darina Trendafilova,Sara G Susco,Daisy Lam,Angelica Messana,Kristina M Holton,Kyle W Karhohs,Beth A Cimini,Kathleen Pfaff,Anne E Carpenter,Lee L Rubin,Lindy E BarrettPubMed 37083703
- Genome biology 24:592023CRISPR screens identify gene targets at breast cancer risk loci.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatasha K Tuano,Jonathan Beesley,Murray Manning,Wei Shi,Laura Perlaza-Jimenez,Luis F Malaver-Ortega,Jacob M Paynter,Debra Black,Andrew Civitarese,Karen McCue,Aaron Hatzipantelis,Kristine Hillman,Susanne Kaufmann,Haran Sivakumaran,Jose M Polo,Roger R Reddel,Vimla Band,Juliet D French,Stacey L Edwards,David R Powell,Georgia Chenevix-Trench,Joseph RosenbluhPubMed 36991492
- Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:78-862023Chinese herbal compound Jinsiwei improves synaptic plasticity in mice with sporadic Alzheimer's disease induced by streptozotocin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Meijing,H E Yannan,Zheng Mingcui,Qin Gaofeng,Gong Zhuoyan,Huang Shuaiyang,Wang PengwenPubMed 36639998
- Journal of clinical laboratory analysis 36:e247512022Identification of Cofilin-1 as a novel biomarker of atopic dermatitis using iTRAQ quantitative proteomics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaotao Zhou,Bo Xiao,Jiajia Zeng,Liying Zhou,Xiaodong Wang,Shangqi Zhao,Xiaobo Li,Huiqiu Zhang,Yanjun Su,Zhenyu Zhao,Xichuan LiPubMed 36305098
- Open medicine (Warsaw, Poland) 17:453-4622022LMO3 promotes proliferation and metastasis of papillary thyroid carcinoma cells by regulating LIMK1-mediated cofilin and the β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeyi Ling,Xiaoli Long,Ying Wu,Jie Li,Mingliang FengPubMed 35350839
- Biological & pharmaceutical bulletin 45:143-1492021Neuroprotective Effects of Tetrahydroxystilbene Glucoside against Rotenone-Induced Toxicity in PC12 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuoqiu Fu,Haiyan Xing,Xianfeng Wang,Yao Liu,Bin Li,Lin Zhang,Ziwei Li,Dongyu Duan,Jianhong ChenPubMed 34707025
- iScience 24:1029932021Vascular smooth muscle cell dysfunction contribute to neuroinflammation and Tau hyperphosphorylation in Alzheimer disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJorge A Aguilar-Pineda,Karin J Vera-Lopez,Pallavi Shrivastava,Miguel A Chávez-Fumagalli,Rita Nieto-Montesinos,Karla L Alvarez-Fernandez,Luis D Goyzueta Mamani,Gonzalo Davila Del-Carpio,Badhin Gomez-Valdez,Clint L Miller,Rajeev Malhotra,Mark E Lindsay,Christian L Lino CardenasPubMed 34505007
- Cancers 13:2021MiR-200c-3p Modulates Cisplatin Resistance in Biliary Tract Cancer by ZEB1-Independent Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlorian Posch,Felix Prinz,Amar Balihodzic,Christian Mayr,Tobias Kiesslich,Christiane Klec,Katharina Jonas,Dominik A Barth,Jakob M Riedl,Armin Gerger,Martin PichlerPubMed 34439151
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Alternative names
CFL, CFL1, Cofilin-1, 18 kDa phosphoprotein, p18
Recommended products
Rabbit Polyclonal Cofilin-1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 80 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Cofilin-1 also known just as cofilin is a small actin-modulating protein with a molecular weight of approximately 19 kDa. It is part of the actin chemical structure regulation and plays an important role in actin filament dynamics by severing and depolymerizing actin filaments. Cofilin-1 expresses widely in both muscle and non-muscle tissues modulating the actin cytoskeleton which is critical for cell movement shape and various signaling pathways. The phosphorylated form of this protein known as phospho-cofilin or p-cofilin is another significant state to consider.
- Biological function summary
Through its interaction with actin filaments cofilin-1 functions to regulate the length and turnover of the actin cytoskeleton within cells. Cofilin-1 is part of a critical complex where it binds to actin monomers and filaments to facilitate the disassembly and recycling of actin. This regulation impacts cellular activities such as migration endocytosis and division influencing how cells respond to internal and external signals. Cofilin-1's ability to bind and sever actin filaments forms an important part of its functional mechanism altering the cytoskeletal architecture for various cellular processes.
- Pathways
Cofilin-1 participates principally in the actin dynamics component of the cytoskeleton remodeling pathway. Within this framework cofilin-1 acts alongside other proteins like actin-depolymerizing factor (ADF) and tropomyosin influencing assembly and disassembly cycles of actin filaments. The Rho family of GTPases regulates cofilin-1 activity through signaling pathways such as the Rho/ROCK/LIMK pathway. This signaling regulation affects actin filament organization impacting cellular processes like axonal guidance and directed cell movement.
- Associated diseases and disorders
There is a strong connection between cofilin-1 dysregulation and neurodegenerative diseases such as Alzheimer's disease and certain types of cancer. In Alzheimer's disease cofilin-1 activity influenced by abnormal cofilin phosphorylation forms part of the pathological mechanisms that lead to synaptic dysfunction and neurofibrillary tangles. In the context of cancer cofilin-1 collaborates with proteins such as RhoA and LIM kinase contributing to cancer cell invasion and metastasis by reorganizing the actin cytoskeleton to facilitate cell motility. Understanding these interactions helps in targeting therapeutic strategies against these conditions.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Western blot - Anti-Cofilin antibody - Loading Control (ab42824)
Cofilin Western blot staining using rabbit Anti-Cofilin antibody
All lanes: Western blot - Anti-Cofilin antibody - Loading Control (ab42824) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 Human embryonic kidney cell line Whole Cell Lysate at 10 µg
Lane 5: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 20 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody - Loading Control (ab42824)
Cofilin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-Cofilin antibody
ab42824 staining Cofilin in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab42824 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cofilin antibody (ab42824)
IHC image of Cofilin staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42824, 1µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - Anti-Cofilin antibody - Loading Control (ab42824)
Cofilin Western blot staining using rabbit Anti-Cofilin antibody
All lanes: Western blot - Anti-Cofilin antibody - Loading Control (ab42824) at 1 µg/mL
All lanes: Western blot - Recombinant Human Cofilin protein (Recombinant Human Cofilin protein ab62958) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Exposure time: 30s
Western blot - Anti-Cofilin antibody - Loading Control (ab42824)
Cofilin Western blot staining using rabbit Anti-Cofilin antibody
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab42824 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
All lanes: Western blot - Anti-Cofilin antibody - Loading Control (ab42824) at 1 µg/mL
Lane 1: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
Lane 2: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 20 kDa
Exposure time: 30s
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab42824)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Cofilin with Anti-Cofilin antibody [RM1210] - Loading Control ab319061 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in PC-12 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Left Panel is applied with Anti-Cofilin antibody [RM1210] (Anti-Cofilin antibody [RM1210] - Loading Control ab319061) at 1/500 dilution (1.036 ug/ml); Right panel is applied with Anti-Cofilin antibody (ab42824) at 1/100 dilution (5.18 ug/ml). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab42824)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Cofilin with Anti-Cofilin antibody [RM1210] - Loading Control ab319061 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Left Panel is applied with Anti-Cofilin antibody [RM1210] (Anti-Cofilin antibody [RM1210] - Loading Control ab319061) at 1/500 dilution (1.036 ug/ml); Right panel is applied with Anti-Cofilin antibody (ab42824) at 1/100 dilution (5.18 ug/ml). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Cofilin antibody (ab42824)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Cofilin with Anti-Cofilin antibody [RM1210] - Loading Control ab319061 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and weak nuclear staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Left Panel is applied with Anti-Cofilin antibody [RM1210] (Anti-Cofilin antibody [RM1210] - Loading Control ab319061) at 1/500 dilution (1.036 ug/ml); Right panel is applied with Anti-Cofilin antibody (ab42824) at 1/100 dilution (5.18 ug/ml). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Magenta). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.
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