Rabbit Recombinant Monoclonal COG7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-COG7 antibody [EPR9942] - BSA and Azide free (AB249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--western-blot-img113031.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-COG7 antibody [EPR9942] - BSA and Azide free (AB249458)")
![Immunocytochemistry/ Immunofluorescence - Anti-COG7 antibody [EPR9942] - BSA and Azide free (AB249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--immunocytochemistry-immunofluorescence-img1815.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-COG7 antibody [EPR9942] - BSA and Azide free (AB249458)")
![Flow Cytometry (Intracellular) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (AB249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--flow-cytometry-intracellular-img25717.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (AB249458)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Tested |
| Mouse | Expected | Expected | Not recommended | Tested | Expected |
| Rat | Expected | Expected | Not recommended | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Alternative names
UNQ3082/PRO10013, COG7, Conserved oligomeric Golgi complex subunit 7, COG complex subunit 7, Component of oligomeric Golgi complex 7
Recommended products
Rabbit Recombinant Monoclonal COG7 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9942
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249458 is the carrier-free version of Anti-COG7 antibody [EPR9942] ab168362.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--western-blot-img113031.jpg "Western blot - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)")
Western blot - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)
This data was developed using Anti-COG7 antibody [EPR9942] ab168362, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458) at 1/1000 dilution
Lane 1: Molt-4 cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: MCF7 cell lysate at 10 µg
Lane 4: C6 cell lysate at 10 µg
Lane 5: RAW 264.7 cell lysate at 10 µg
Lane 6: PC-12 cell lysate at 10 µg
Lane 7: NIH/3T3 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 86 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137567.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)
This data was developed using Anti-COG7 antibody [EPR9942] ab168362, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling COG7 with Anti-COG7 antibody [EPR9942] ab168362 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138475.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)
This data was developed using Anti-COG7 antibody [EPR9942] ab168362, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COG7 with Anti-COG7 antibody [EPR9942] ab168362 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--immunocytochemistry-immunofluorescence-img1815.jpg "Immunocytochemistry/ Immunofluorescence - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)")
Immunocytochemistry/ Immunofluorescence - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)
This data was developed using Anti-COG7 antibody [EPR9942] ab168362, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling COG7 with Anti-COG7 antibody [EPR9942] ab168362 at 1/100 dilution. DAPI nuclear staining (blue).
![Flow Cytometry (Intracellular) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458), expandable thumbnail](https://content.abcam.com/products/images/cog7-antibody-epr9942-bsa-and-azide-free-ab249458--flow-cytometry-intracellular-img25717.jpg "Flow Cytometry (Intracellular) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)")
Flow Cytometry (Intracellular) - Anti-COG7 antibody [EPR9942] - BSA and Azide free (ab249458)
This data was developed using Anti-COG7 antibody [EPR9942] ab168362, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling COG7 (red) with Anti-COG7 antibody [EPR9942] ab168362 at 1/10 dilution, compared to a nonspecific control antibody (green).
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