Anti-Coilin antibody

• WB

Lab

Western blot - Anti-Coilin antibody (AB210785)

Western blot : Anti-COIL antibody (ab210785) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab210785 was shown to bind specifically to COIL. A band was observed at 68 kDa in wild-type HeLa cell lysates with no signal observed at this size in COIL knockout cell line. To generate this image, wild-type and COIL knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Coilin antibody (ab210785) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lanes 1 - 4:

Western blot - Human COIL (Coilin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-coil-coilin-knockout-hela-cell-line-ab261757'>ab261757</a>)

Lane 2:

COIL knockout HeLa cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 68 kDa

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• WB

Supplier Data

Western blot - Anti-Coilin antibody (AB210785)

All lanes:

Western blot - Anti-Coilin antibody (ab210785) at 1/500 dilution

Lane 1:

MCF7 cell line extract

Lane 2:

HepG2 cell line extract

Lane 3:

293T cell line extract

Lane 4:

Rat testis lysate

Predicted band size: 62 kDa

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