Rabbit Polyclonal COL1A2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 52 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A2 aa 1050-1350.
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- Materials today. Bio 20:1006162023Photocatalytic oxygen evolution and antibacterial biomimetic repair membrane for diabetes wound repair via HIF1-α pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanlin Su,Bing Ye,Ziming Zhang,Qing Gao,Lian Zeng,Yizhou Wan,Wenzhe Sun,Siyue Chen,Daping Quan,Jialin Yu,Xiaodong GuoPubMed 37025556
- EMBO molecular medicine 15:e168342023Bi-allelic mutation in SEC16B alters collagen trafficking and increases ER stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAhmed El-Gazzar,Barbara Voraberger,Frank Rauch,Mario Mairhofer,Katy Schmidt,Brecht Guillemyn,Goran Mitulović,Veronika Reiterer,Margot Haun,Michaela M Mayr,Johannes A Mayr,Susanne Kimeswenger,Oliver Drews,Vrinda Saraff,Nick Shaw,Nadja Fratzl-Zelman,Sofie Symoens,Hesso Farhan,Wolfgang HöglerPubMed 36916446
- Molecular carcinogenesis 62:438-4492022Mesothelioma cancer cells are glutamine addicted and glutamine restriction reduces YAP1 signaling to attenuate tumor formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGautam Adhikary,Suruchi Shrestha,Warren Naselsky,John J Newland,Xi Chen,Wen Xu,Ashkan Emadi,Joseph S Friedberg,Richard L EckertPubMed 36562471
- Frontiers in cell and developmental biology 10:9995472022Survivin knockdown alleviates pathological hydrostatic pressure-induced bladder smooth muscle cell dysfunction and BOO-induced bladder remodeling autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuo Chen,Shuang Chen,Xingpeng Di,Shengyin He,Yugao Liu,Rui Qu,Yi Luo,Yuebai Liu,Luo YangPubMed 36393846
- Human gene (Amsterdam, Netherlands) 34:2010932022Role of circulatory miRNA-21 and associated signaling pathways in the pathogenesis of pulmonary fibrosis among individuals recovered after COVID-19 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohammad Ali,Fahed Abdullah,Aleemuddin Naveed,Syed Mahmood Ahmed,Aleem Ahmed Khan,Ashfaq HasanPubMed 37521444
- Scientific reports 12:124742022Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Ju,Hiroki Hayashi,Munehisa Shimamura,Satoshi Baba,Shota Yoshida,Ryuichi Morishita,Hiromi Rakugi,Hironori NakagamiPubMed 35864207
- Journal of dental sciences 17:1281-12912022MicroRNA-34a and microRNA-146a target CELF3 and suppress the osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianmin Meng,Wenjie Wang,Xueling WangPubMed 35784124
- PloS one 17:e02663742022Hsa_circ_0056686, derived from cancer-associated fibroblasts, promotes cell proliferation and suppresses apoptosis in uterine leiomyoma through inhibiting endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeifang Suo,Zhichen Lin,Dongfang Guo,Airong ZhangPubMed 35390056
- International journal of molecular sciences 23:2022Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatia Pane,Cristina Quintavalle,Silvia Nuzzo,Francesco Ingenito,Giuseppina Roscigno,Alessandra Affinito,Iolanda Scognamiglio,Birlipta Pattanayak,Enrico Gallo,Antonella Accardo,Guglielmo Thomas,Zoran Minic,Maxim V Berezovski,Monica Franzese,Gerolama CondorelliPubMed 35409352
- Frontiers in bioengineering and biotechnology 10:8524822022Porous Se@SiO Nanoparticles Enhance Wound Healing by ROS-PI3K/Akt Pathway in Dermal Fibroblasts and Reduce Scar Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo-Yu Yang,Zhi-Yuan Zhou,Shi-Yun Liu,Ming-Jun Shi,Xi-Jian Liu,Tian-Ming Cheng,Guo-Ying Deng,Ye Tian,Jian Song,Xuan-Hao LiPubMed 35387298
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human COL1A2 aa 1050-1350. The exact immunogen used to generate this antibody is proprietary information. Database link P08123
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog, Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Dog, Monkey | Dilution info - | Notes - |
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2
Recommended products
Rabbit Polyclonal COL1A2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 52 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A2 aa 1050-1350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human COL1A2 aa 1050-1350. The exact immunogen used to generate this antibody is proprietary information. Database link P08123
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-COL1A2 antibody (ab96723)
7.5% SDS PAGE
All lanes: Western blot - Anti-COL1A2 antibody (ab96723) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Predicted band size: 129 kDa
Immunocytochemistry/ Immunofluorescence - Anti-COL1A2 antibody (ab96723)
Immunofluorescence analysis of COL1A2 in paraformaldehyde fixed A431 cell, using ab96723 at a 1/200 dilution.
Lower image: merged with DNA probe.
Immunocytochemistry/ Immunofluorescence - Anti-COL1A2 antibody (ab96723)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WI-38 (human fetal lung fibroblast) cells labelling COL1A2 with Anti-COL1A2 antibody [RM1295] ab322963 at 1/100 (5.11 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in WI-38 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control: HeLa.
Left Panel is applied with Anti-COL1A2 antibody [RM1295] (Anti-COL1A2 antibody [RM1295] ab322963) at 1/100 dilution (5.11 ug/ml);
Right panel is applied with Anti-COL1A2 antibody (ab96723) at 1/100 dilution (13.3 ug/ml).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-COL1A2 antibody (ab96723)
COL1A2 western blot using anti-COL1A2 antibody ab96723. Publication image and figure legend from Chen, H., Xu, H., et al., 2020, Front Pharmacol, PubMed 31920668.
ab96723 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab96723 please see the product overview.
Effects of thalidomide on mRNA and protein expression related to fibrosis in mice induced with TNBS. (A) QRT-PCR assay was performed to evaluate the expression levels of fibrosis-related mRNA in mice from different groups. (B) Western blot assay was performed to evaluate the expression levels of fibrosis-related proteins in mice from different groups. **P < 0.01 compared with the sham-1 and sham-2 groups; #P < 0.05, ##P < 0.01 compared with the model group.
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