Anti-COL1A2 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COL1A2 antibody (AB96723)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WI-38 (human fetal lung fibroblast) cells labelling COL1A2 with ab322963 at 1/100 (5.11 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in WI-38 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : HeLa.
Left Panel is applied with Anti-COL1A2 antibody [RM1295] (ab322963) at 1/100 dilution (5.11 ug/ml);
Right panel is applied with Anti-COL1A2 antibody (ab96723) at 1/100 dilution (13.3 ug/ml).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-COL1A2 antibody (AB96723)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-COL1A2 antibody (ab96723) at 1/1000 dilution

Lane 1:

U87-MG whole cell extract at 30 µg

Lane 2:

U87-MG whole cell extract (conditioned medium) at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 150 kDa

false

• WB

Supplier Data

Western blot - Anti-COL1A2 antibody (AB96723)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-COL1A2 antibody (ab96723) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts

Lane 2:

Transfected 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 150 kDa

false

• WB

Supplier Data

Western blot - Anti-COL1A2 antibody (AB96723)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-COL1A2 antibody (ab96723) at 1/1000 dilution

Lane 1:

Zebrafish muscle tissue extract at 30 µg

Lane 2:

Whole zebrafish tissue extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 150 kDa

false

• WB

CiteAb

Western blot - Anti-COL1A2 antibody (AB96723)

COL1A2 western blot using anti-COL1A2 antibody ab96723. Publication image and figure legend from Chen, H., Xu, H., et al., 2020, Front Pharmacol, PubMed 31920668.

ab96723 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab96723 please see the product overview.

Effects of thalidomide on mRNA and protein expression related to fibrosis in mice induced with TNBS. (A) QRT-PCR assay was performed to evaluate the expression levels of fibrosis-related mRNA in mice from different groups. (B) Western blot assay was performed to evaluate the expression levels of fibrosis-related proteins in mice from different groups. **p < 0.01 compared with the sham-1 and sham-2 groups; #p < 0.05, ##p < 0.01 compared with the model group.

false