Rabbit Recombinant Monoclonal COL1A2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994), expandable thumbnail](https://content.abcam.com/products/images/col1a2-antibody-epr24262-52-ab270994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106705.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994), expandable thumbnail](https://content.abcam.com/products/images/col1a2-antibody-epr24262-52-ab270994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124035.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994)")
Publications
Filter by
- Cells 11:2022Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAsima Tayyeb,Gry H Dihazi,Björn Tampe,Michael Zeisberg,Desiree Tampe,Samy Hakroush,Charlotte Bührig,Jenny Frese,Nazli Serin,Marwa Eltoweissy,Gerhard A Müller,Hassan DihaziPubMed 35456008
- Cancer management and research 13:8929-89512021Bioinformatics Analysis of Hub Genes and Potential Therapeutic Agents Associated with Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiyu Zhang,Xuelian Xiang,Li Liu,Huiying Yang,Dongliang Cen,Guodu TangPubMed 34876855
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | IHC-P | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2
Recommended products
Rabbit Recombinant Monoclonal COL1A2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24262-52
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (ab270994), expandable thumbnail](https://content.abcam.com/products/images/col1a2-antibody-epr24262-52-ab270994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106705.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (ab270994)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (ab270994)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling COL1A2 with ab270994 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on on stromal cells of human colon. The section was incubated with ab270994 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX® instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (ab270994), expandable thumbnail](https://content.abcam.com/products/images/col1a2-antibody-epr24262-52-ab270994--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124035.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (ab270994)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (ab270994)
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling COL1A2 with ab270994 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human colon adenocarcinoma. The section was incubated with ab270994 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX® instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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