AB270994

Anti-COL1A2 antibody [EPR24262-52]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal COL1A2 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling COL1A2 with ab270994 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human colon adenocarcinoma. The section was incubated with ab270994 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX^® instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling COL1A2 with ab270994 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on on stromal cells of human colon. The section was incubated with ab270994 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX^® instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-COL1A2 antibody [EPR24262-52]
578 PE

PE Anti-COL1A2 antibody [EPR24262-52]
660 APC

APC Anti-COL1A2 antibody [EPR24262-52]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-COL1A2 antibody [EPR24262-52]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-COL1A2 antibody [EPR24262-52]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-COL1A2 antibody [EPR24262-52]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-COL1A2 antibody [EPR24262-52]
Carrier free

Anti-COL1A2 antibody [EPR24262-52] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24262-52

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information COL1A2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of pathology 267:79-91 PubMed40719154

2025

Spatial transcriptomics identifies SPARC as a prognostic marker in interstitial lung diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Niitsu,Toshiaki Kataoka,Kiyoharu Fukushima,Daisuke Motooka,Shigeyuki Shichino,Yayoi Natsume-Kitatani,Hideya Kitamura,Takashi Niwa,Tomohisa Baba,Daisuke Okuzaki,Atsushi Kumanogoh,Shizuo Akira,Koji Okudela,Takashi Ogura

Cells 11: PubMed35456008

2022

Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Asima Tayyeb,Gry H Dihazi,Björn Tampe,Michael Zeisberg,Desiree Tampe,Samy Hakroush,Charlotte Bührig,Jenny Frese,Nazli Serin,Marwa Eltoweissy,Gerhard A Müller,Hassan Dihazi

Cancer management and research 13:8929-8951 PubMed34876855

2021

Bioinformatics Analysis of Hub Genes and Potential Therapeutic Agents Associated with Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Zhang,Xuelian Xiang,Li Liu,Huiying Yang,Dongliang Cen,Guodu Tang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COL1A2 antibody [EPR24262-52]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL1A2 antibody [EPR24262-52] (AB270994)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise