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Rabbit Recombinant Monoclonal COL1A2 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Mouse samples.

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Images

Western blot - Anti-COL1A2 antibody [EPR29717-534] (AB323563), expandable thumbnail
  • Western blot - Anti-COL1A2 antibody [EPR29717-534] (AB323563), expandable thumbnail
  • Western blot - Anti-COL1A2 antibody [EPR29717-534] (AB323563), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Mouse
Tested
Not recommended
Recombinant fragment - Human
Tested
Not recommended

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant fragment - Human
Dilution info
-
Notes

-

Target data

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Alternative names

Cola2, Collagen alpha-2(I) chain, Alpha-2 type I collagen

Rabbit Recombinant Monoclonal COL1A2 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29717-534
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563), expandable thumbnail

    Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with recombinant human COL1A1, COL2A1, COL3A1 or COL5A2 by Western Blot.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563) at 1/1000 dilution

    Lane 1: His-tagged human COL1A2 recombinant protein at 10 ng

    Lane 2: His-tagged human COL2A1 recombinant protein at 10 ng

    Lane 3: His-tagged human COL1A1 recombinant protein at 10 ng

    Lane 4: His-tagged human COL3A1 recombinant protein at 10 ng

    Lane 5: His-tagged human COL5A2 recombinant protein at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 28 kDa

    Exposure time: 26s

  • Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563), expandable thumbnail

    Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: brain

    Cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563) at 1/1000 dilution

    Lane 1: Mouse articular cartilage tissue lysate at 40 µg

    Lane 2: Mouse bone cartilage tissue lysate at 40 µg

    Lane 3: Mouse skin tissue lysate at 40 µg

    Lane 4: Mouse brain tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 35 kDa, 165 kDa, 36 kDa

    Exposure time: 37s

  • Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563), expandable thumbnail

    Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: RAW 264.7

    Cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-COL1A2 antibody [EPR29717-534] (ab323563) at 1/1000 dilution

    Lane 1: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 35,165 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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