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Rabbit Monoclonal COL1A2 antibody. Carrier free. Suitable for WB and reacts with Human, Recombinant fragment, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A2.

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Images

Western blot - Anti-COL1A2 antibody [HL2049] (AB308221), expandable thumbnail
  • Western blot - Anti-COL1A2 antibody [HL2049] (AB308221), expandable thumbnail
  • Western blot - Anti-COL1A2 antibody [HL2049] (AB308221), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human COL1A2. The exact immunogen used to generate this antibody is proprietary information. Database link P08123

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
Recombinant fragment
Tested

Tested
Tested

Species
Human
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Recombinant fragment
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Mouse
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Species
Rat
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Target data

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Alternative names

Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2

Recommended products

Rabbit Monoclonal COL1A2 antibody. Carrier free. Suitable for WB and reacts with Human, Recombinant fragment, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A2.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human COL1A2. The exact immunogen used to generate this antibody is proprietary information. Database link P08123
Carrier free
Yes
Clone number
HL2049
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-COL1A2 antibody [HL2049] (ab308221), expandable thumbnail

    Western blot - Anti-COL1A2 antibody [HL2049] (ab308221)

    Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (ab308221) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: Non-transfected 293T cell lysates

    Lane 2: DDDDK-tagged COL1A1, 293T cell lysates

    Lane 3: DDDDK-tagged COL1A2, 293T cell lysates

    Lane 4: DDDDK-tagged COL3A1, 293T cell lysates

  • Western blot - Anti-COL1A2 antibody [HL2049] (ab308221), expandable thumbnail

    Western blot - Anti-COL1A2 antibody [HL2049] (ab308221)

    U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (ab308221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-COL1A2 antibody [HL2049] (ab308221) at 1/1000 dilution

    Lane 1: U87-MG cell lysate at 30 µg

    Lane 2: U87-MG cell lysate with conditioned medium at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-COL1A2 antibody [HL2049] (ab308221), expandable thumbnail

    Western blot - Anti-COL1A2 antibody [HL2049] (ab308221)

    Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (ab308221) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

    Lane 1: Mouse muscle tissue lysate at 50 µg

    Lane 2: Rat muscle lysate at 50 µg

    Developed using the ECL technique.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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