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AB308221

Anti-COL1A2 antibody [HL2049]

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Rabbit Recombinant Monoclonal COL1A2 antibody. Carrier free. Suitable for WB and reacts with Human, Recombinant fragment, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A2.

View Alternative Names

Collagen alpha-2(I) chain, Alpha-2 type I collagen, COL1A2

3 Images
Western blot - Anti-COL1A2 antibody [HL2049] (AB308221)
  • WB

Supplier Data

Western blot - Anti-COL1A2 antibody [HL2049] (AB308221)

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (ab308221) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-COL1A2 antibody [HL2049] (ab308221) at 1/1000 dilution

Lane 1:

U87-MG cell lysate at 30 µg

Lane 2:

U87-MG cell lysate with conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-COL1A2 antibody [HL2049] (AB308221)
  • WB

Supplier Data

Western blot - Anti-COL1A2 antibody [HL2049] (AB308221)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (ab308221) diluted at 1 : 500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lane 1:

Non-transfected 293T cell lysates

Lane 2:

DDDDK-tagged COL1A1, 293T cell lysates

Lane 3:

DDDDK-tagged COL1A2, 293T cell lysates

Lane 4:

DDDDK-tagged COL3A1, 293T cell lysates

false

Western blot - Anti-COL1A2 antibody [HL2049] (AB308221)
  • WB

Supplier Data

Western blot - Anti-COL1A2 antibody [HL2049] (AB308221)

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (ab308221) diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Lane 1:

Mouse muscle tissue lysate at 50 µg

Lane 2:

Rat muscle lysate at 50 µg

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2049

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human COL1A2. The exact immunogen used to generate this antibody is proprietary information.

P08123

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information COL1A2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com