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AB125208

Anti-COL4A2 antibody

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(4 Publications)

Rabbit Polyclonal CO4A2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human Collagen alpha-2(IV) chain.

View Alternative Names

Collagen alpha-2(IV) chain, COL4A2

1 Images
Western blot - Anti-COL4A2 antibody (AB125208)
  • WB

Supplier Data

Western blot - Anti-COL4A2 antibody (AB125208)

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT.

The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

All lanes:

Western blot - Anti-COL4A2 antibody (ab125208) at 0.25 µg/mL

All lanes:

Human placenta tissue lysates at 30 µg

Secondary

All lanes:

Goat anti-rabbit IgG-HRP secondary antibody at 1/5000 dilution

Predicted band size: 168 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Collagen alpha-2(IV) chain. The exact immunogen used to generate this antibody is proprietary information.

P08572

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Constituents: 76.92% Trehalose, 17.31% Sodium chloride, 3.85% Disodium hydrogenorthophosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
See full target information Collagen alpha-2(IV) chain
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Genome medicine 16:98 PubMed39138551

2024

Spatial multiomics reveals a subpopulation of fibroblasts associated with cancer stemness in human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Yu Jing,Dan Liu,Na Feng,Hui Dong,He-Qi Wang,Xi Yan,Xu-Feng Chen,Min-Cheng Qu,Ping Lin,Bin Yi,Feiling Feng,Lei Chen,Hong-Yang Wang,Hong Li,Yu-Fei He

Oncology letters 27:225 PubMed38586200

2024

Molecular mechanism of formation and destruction of a pseudo‑capsule in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takuto Shimizu,Makito Miyake,Kota Iida,Sayuri Onishi,Tomomi Fujii,Yusuke Iemura,Kazuki Ichikawa,Chihiro Omori,Fumisato Maesaka,Mitsuru Tomizawa,Tatsuki Miyamoto,Nobumichi Tanaka,Kiyohide Fujimoto

Journal of neuro-oncology 161:415-423 PubMed36811765

2023

Type IV collagen-derived angiogenesis inhibitor: canstatin low expressing in brain-invasive meningiomas using liquid chromatography-mass spectrometry (LC-MS/MS).

Applications

Unspecified application

Species

Unspecified reactive species

Jian Pei,Pei Li,Yun H Gao,Bao G Tian,Da Y Wang,Yu Zheng,Li Y Liu,Zhi Y Zhang,Si S Huang,Min Wen,Xiang Xu,Lei Xia

Oncogene 41:4336-4348 PubMed35945453

2022

Forming cytoplasmic stress granules PURα suppresses mRNA translation initiation of IGFBP3 to promote esophageal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Lusong Tian,Xiufeng Xie,Urmi Das,Yuling Chen,Yulin Sun,Fang Liu,Haizhen Lu,Peng Nan,Ying Zhu,Xinglu Gu,Haiteng Deng,Jiuyong Xie,Xiaohang Zhao
View all publications
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