Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling COL4A2 with ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on blood vessels of human cerebrum. The section was incubated with ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COL4A2 with ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on stroma of human kidney (PMID : 31630546). The section was incubated with ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling COL4A2 with ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat lung. The section was incubated with ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling COL4A2 with ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse lung. The section was incubated with ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling COL4A2 with ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on blood vessels of mouse cerebrum. The section was incubated with ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining COL4A2 with ab316099 at a 1/500 dilution, ab307696 anti-ALDH1L1 used at 1/5000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Panel A : merged staining of anti-COL4A2 (green; Opal^™520), anti-ALDH1L1 (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on rat liver.
Panel B : anti-COL4A2 staining endothelium in rat liver.
Panel C : anti-ALDH1L1 staining hepatocytes in rat liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in rat liver.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316099, ab307696 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining COL4A2 with ab316099 at a 1/500 dilution, ab307696 anti-ALDH1L1 used at 1/5000 dilution and ab52625 anti-Cytokeratin 19 used at a 1/6000 dilution.

Panel A : merged staining of anti-COL4A2 (green; Opal^™520), anti-ALDH1L1 (magenta; Opal^™690) and anti-Cytokeratin 19 (grey; Opal^™570) on mouse liver.
Panel B : anti-COL4A2 staining endothelium in mouse liver.
Panel C : anti-ALDH1L1 staining hepatocytes in mouse liver.
Panel D : anti-Cytokeratin 19 staining branch of bile ducts in mouse liver.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316099, ab307696 and ab52625 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling COL4A2 with ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on blood vessels of rat cerebrum. The section was incubated with ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)

This data was developed using ab316099, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human esophagus tissue staining Eph receptor A2 with ab324955 at a 1/50 (10.12 μg/ml) dilution, ab316099 anti-COL4A2 used at a 1/500 (1.07 μg/ml) dilution and ab321976 anti-LAMA3 used at a 1/100 (4.88 μg/ml) dilution.

Panel A : anti-Eph receptor A2 (green; Opal™520), anti-COL4A2 (magenta; Opal™690), anti-LAMA3 (gray; Opal™570) on human esophagus.

Panel B : anti-Eph receptor A2 staining epithelium in human esophagus.

Panel C : anti-COL4A2 staining endothelium and smooth muscle in human esophagus.

Panel D : anti-LAMA3 staining basement membrane in human esophagus.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324955, ab316099 and ab321976 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.