Rabbit Recombinant Monoclonal CO4A2 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428721.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428722.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428719.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428720.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428717.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (AB316100)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt | IP | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
Alternative names
Collagen alpha-2(IV) chain, COL4A2
Recommended products
Rabbit Recombinant Monoclonal CO4A2 antibody. Carrier free. Suitable for IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25363-49
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316100 is the carrier-free version of Anti-COL4A2 antibody [EPR25363-49] ab316099.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428721.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)
This data was developed using Anti-COL4A2 antibody [EPR25363-49] ab316099, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling COL4A2 with Anti-COL4A2 antibody [EPR25363-49] ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on blood vessels of rat cerebrum. The section was incubated with Anti-COL4A2 antibody [EPR25363-49] ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428722.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)
This data was developed using Anti-COL4A2 antibody [EPR25363-49] ab316099, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling COL4A2 with Anti-COL4A2 antibody [EPR25363-49] ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat lung. The section was incubated with Anti-COL4A2 antibody [EPR25363-49] ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428719.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)
This data was developed using Anti-COL4A2 antibody [EPR25363-49] ab316099, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling COL4A2 with Anti-COL4A2 antibody [EPR25363-49] ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on blood vessels of mouse cerebrum. The section was incubated with Anti-COL4A2 antibody [EPR25363-49] ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428720.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)
This data was developed using Anti-COL4A2 antibody [EPR25363-49] ab316099, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling COL4A2 with Anti-COL4A2 antibody [EPR25363-49] ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse lung. The section was incubated with Anti-COL4A2 antibody [EPR25363-49] ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428717.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)
This data was developed using Anti-COL4A2 antibody [EPR25363-49] ab316099, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling COL4A2 with Anti-COL4A2 antibody [EPR25363-49] ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on blood vessels of human cerebrum. The section was incubated with Anti-COL4A2 antibody [EPR25363-49] ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428718.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)
This data was developed using Anti-COL4A2 antibody [EPR25363-49] ab316099, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling COL4A2 with Anti-COL4A2 antibody [EPR25363-49] ab316099 at 1/500 (1.07 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of human kidney (PMID: 31630546). The section was incubated with Anti-COL4A2 antibody [EPR25363-49] ab316099 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100), expandable thumbnail](https://content.abcam.com/products/images/col4a2-antibody-epr25363-49-bsa-and-azide-free-ab316100--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428718.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A2 antibody [EPR25363-49] - BSA and Azide free (ab316100)")
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