Rabbit Polyclonal COL4A5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Collagen alpha-5(IV) chain aa 1450 to C-terminus.
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Publications
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- Frontiers in cellular neuroscience 17:11917402023MiR-29a-deficiency causes thickening of the basilar membrane and age-related hearing loss by upregulating collagen IV and laminin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Ma,Shuli Wang,Ruishuang Geng,Yongfeng Gong,Mulan Li,Daoli Xie,Yaning Dong,Tihua Zheng,Bo Li,Tong Zhao,Qingyin ZhengPubMed 37275774
- Open research Europe 2:872022Methodological approaches in aggregate formation and microscopic analysis to assess pseudoislet morphology and cellular interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFredrik Wieland,Anika Schumacher,Nadia Roumans,Clemens van Blitterswijk,Vanessa LaPointe,Timo RademakersPubMed 37645341
- Annals of translational medicine 9:14202021Functional assessment of a novel splicing site variant in a Chinese X-linked Alport syndrome family.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolei Chen,Nan Ye,Lu Zhang,Wen Zheng,Jingqiu Cheng,Meng GongPubMed 34733972
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Collagen alpha-5(IV) chain aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29400
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Dog | Predicted | Predicted |
| Pig | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Pig | Dilution info 0.20000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 0.20000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-20.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Pig | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Alternative names
Collagen alpha-5(IV) chain, COL4A5
Recommended products
Rabbit Polyclonal COL4A5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Collagen alpha-5(IV) chain aa 1450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Collagen alpha-5(IV) chain aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P29400
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-COL4A5 antibody (ab231957)
All lanes: Western blot - Anti-COL4A5 antibody (ab231957) at 2 µg/mL
All lanes: Pig cerebrum lysate.
Developed using the ECL technique.
Predicted band size: 161 kDa
Western blot - Anti-COL4A5 antibody (ab231957)
All lanes: Western blot - Anti-COL4A5 antibody (ab231957) at 2 µg/mL
All lanes: Mouse cerebrum lysate.
Developed using the ECL technique.
Predicted band size: 161 kDa
Western blot - Anti-COL4A5 antibody (ab231957)
All lanes: Western blot - Anti-COL4A5 antibody (ab231957) at 2 µg/mL
All lanes: Recombinant human COL4A5 protein.
Developed using the ECL technique.
Predicted band size: 161 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A5 antibody (ab231957)
Formalin-fixed, paraffin-embedded human kidney tissue stained for COL4A5 usign ab231957 at 20 μg/mL in immunohistochemical analysis. DAB staining.
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