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AB231957

Anti-COL4A5 antibody

2

(1 Review)

|

(8 Publications)

Rabbit Polyclonal COL4A5 antibody. Suitable for WB, IHC-P and reacts with Mouse, Pig, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Collagen alpha-5(IV) chain aa 1450 to C-terminus.

View Alternative Names

Collagen alpha-5(IV) chain, COL4A5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A5 antibody (AB231957)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL4A5 antibody (AB231957)

Formalin-fixed, paraffin-embedded human kidney tissue stained for COL4A5 usign ab231957 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-COL4A5 antibody (AB231957)
  • WB

Supplier Data

Western blot - Anti-COL4A5 antibody (AB231957)

All lanes:

Western blot - Anti-COL4A5 antibody (ab231957) at 2 µg/mL

All lanes:

Recombinant human COL4A5 protein.

Predicted band size: 161 kDa

true

Western blot - Anti-COL4A5 antibody (AB231957)
  • WB

Supplier Data

Western blot - Anti-COL4A5 antibody (AB231957)

All lanes:

Western blot - Anti-COL4A5 antibody (ab231957) at 2 µg/mL

All lanes:

Pig cerebrum lysate.

Predicted band size: 161 kDa

true

Western blot - Anti-COL4A5 antibody (AB231957)
  • WB

Supplier Data

Western blot - Anti-COL4A5 antibody (AB231957)

All lanes:

Western blot - Anti-COL4A5 antibody (ab231957) at 2 µg/mL

All lanes:

Mouse cerebrum lysate.

Predicted band size: 161 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Collagen alpha-5(IV) chain aa 1450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P29400

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Alternative Products

Primary Antibodies

AB157779

Anti-COL4A5 antibody

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Complementary Products

Assay Kits

AB222942

Total Collagen Assay Kit (Perchlorate-Free)

Functional Studies - Total Collagen Assay Kit (Perchlorate-Free) (AB222942)

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Primary Antibodies

AB236640

Anti-Collagen IV antibody [EPR22911-127]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen IV antibody [EPR22911-127] (AB236640)

  • 5
  • 2
Primary Antibodies

AB316099

Anti-COL4A2 antibody [EPR25363-49]

BOND RX™ Validated|Advanced Validation|20ul selling size|Recombinant|RabMAb|Trial Size

Multiplex immunohistochemistry - Anti-COL4A2 antibody [EPR25363-49] (AB316099)

  • 0
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB111742

Anti-Collagen Type IV alpha 3 (COL4a3) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen Type IV alpha 3 (COL4a3) antibody (AB111742)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
See full target information Collagen alpha-5(IV) chain
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular genetics & genomic medicine 13:e70053 PubMed39924725

2025

Effects of a Novel COL4A3 Homozygous/Heterozygous Splicing Mutation on the Mild Phenotype in a Family With Autosomal Recessive Alport Syndrome and a Literature Review.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Chen,Li Zhang,Jing Rao,Yan Zhou,Lujun Dai,Songsong Huang,Chunxia Yang,Qiuhan Bian,Tao Zhang,Xiaoyan Yang

Scientific reports 14:24426 PubMed39424871

2024

Machine learning-based model for CD4 conventional T cell genes to predict survival and immune responses in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zijing Wang,Zhanyuan Sun,Hengyi Lv,Wenjun Wu,Hai Li,Tao Jiang

Clinical kidney journal 17:sfae037 PubMed38455522

2024

Characterization of glomerular basement membrane components within pediatric glomerular diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Chen,Xindi Zhou,Chun Gan,Qing Yang,Wanbing Chen,Xiaoqian Feng,Tao Zhang,Li Zhang,Lujun Dai,Yaxi Chen,Haiping Yang,Mo Wang,Wei Jiang,Qiu Li

Frontiers in cellular neuroscience 17:1191740 PubMed37275774

2023

MiR-29a-deficiency causes thickening of the basilar membrane and age-related hearing loss by upregulating collagen IV and laminin.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Ma,Shuli Wang,Ruishuang Geng,Yongfeng Gong,Mulan Li,Daoli Xie,Yaning Dong,Tihua Zheng,Bo Li,Tong Zhao,Qingyin Zheng

Advanced healthcare materials 12:e2203021 PubMed37057819

2023

Bioprinting of Stem Cell Spheroids Followed by Post-Printing Chondrogenic Differentiation for Cartilage Tissue Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Monize Caiado Decarli,Adrián Seijas-Gamardo,Francis L C Morgan,Paul Wieringa,Matthew B Baker,Jorge Vicente L Silva,Ângela Maria Moraes,Lorenzo Moroni,Carlos Mota

Open research Europe 2:87 PubMed37645341

2022

Methodological approaches in aggregate formation and microscopic analysis to assess pseudoislet morphology and cellular interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Fredrik Wieland,Anika Schumacher,Nadia Roumans,Clemens van Blitterswijk,Vanessa LaPointe,Timo Rademakers

Experimental & molecular medicine 54:653-661 PubMed35596061

2022

Loss of RTN3 phenocopies chronic kidney disease and results in activation of the IGF2-JAK2 pathway in proximal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liang-Liang Fan,Ran Du,Ji-Shi Liu,Jie-Yuan Jin,Chen-Yu Wang,Yi Dong,Wan-Xia He,Ri-Qiang Yan,Rong Xiang

Annals of translational medicine 9:1420 PubMed34733972

2021

Functional assessment of a novel splicing site variant in a Chinese X-linked Alport syndrome family.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolei Chen,Nan Ye,Lu Zhang,Wen Zheng,Jingqiu Cheng,Meng Gong
View all publications
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Product promise

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