Anti-COL6A1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL6A1 antibody (AB151422)

Immunohistochemical analysis of paraffin-embedded hepatoma tissue stained for COL6A1 protein at cytosol on human hepatoma with ab151422 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COL6A1 antibody (AB151422)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue stained for COL6A1 protein at cytosol on mouse liver with ab151422 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COL6A1 antibody (AB151422)

Immunofluorescence analysis of DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling COL6A1 protein using ab151422 at a 1/500 dilution (Green). beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

• WB

Supplier Data

Western blot - Anti-COL6A1 antibody (AB151422)

Samples were separated by 7.5 % SDS. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-COL6A1 antibody (ab151422) at 1/500 dilution

All lanes:

U87-MG whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 105 kDa

false

• WB

Lab

Western blot - Anti-COL6A1 antibody (AB151422)

All lanes:

Western blot - Anti-COL6A1 antibody (ab151422) at 1/500 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

COL6A1 Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 109 kDa

false