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AB278063

Anti-COLEC11 antibody [821]

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Mouse Monoclonal COLEC11 antibody. Suitable for ELISA, WB, ICC/IF and reacts with Transfected cell line - Human, Transfected cell lysate - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human COLEC11 aa 100 to C-terminus.

View Alternative Names

UNQ596/PRO1182, COLEC11, Collectin-11, Collectin kidney protein 1, CL-K1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-COLEC11 antibody [821] (AB278063)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-COLEC11 antibody [821] (AB278063)

Immunocytochemical analysis of CHO cells labeling collectin-11 with ab278063 at 1/500 dilution (Green). The FITC-conjugate rabbit anti-mouse IgG at 1/4000 dilution was added. Nucleus (DNA) was stained with DAPI (Blue). On panel A CHO cells were expressing human recombinant Collectin-11. On panel B CHO cells were mock infected.

ELISA - Anti-COLEC11 antibody [821] (AB278063)
  • ELISA

Supplier Data

ELISA - Anti-COLEC11 antibody [821] (AB278063)

The indicated amounts of recombinant Collectin-11 protein expressed in CHO cells were coated onto the wells of the ELISA plate. After blocking with 5% skim milk, ab278063 at 1/1000 dilution was added to each well. HRP-conjugated goat anti-mouse IgG 100 μl, at 1/4000 dilution was added. As substrate, orthophenylenediamine was used. Optical density (OD) measured at 490nm.

Western blot - Anti-COLEC11 antibody [821] (AB278063)
  • WB

Supplier Data

Western blot - Anti-COLEC11 antibody [821] (AB278063)

All lanes:

Western blot - Anti-COLEC11 antibody [821] (ab278063) at 1/500 dilution

All lanes:

Supernatant of CHO cells expressing human recombinant Collectin-11 at 1 µg/mL

Predicted band size: 29 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

821

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, ELISA

applications

Immunogen

Recombinant Fragment Protein within Human COLEC11 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BWP8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Tissue culture supernatant.
Storage buffer
pH: 5 - 8 Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lectin that plays a role in innate immunity, apoptosis and embryogenesis (PubMed : 21258343, PubMed : 23954398, PubMed : 25912189). Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope (PubMed : 25912189). Primarily recognizes the terminal disaccharide of the glycan (PubMed : 25912189). Also recognizes a subset of fucosylated glycans and lipopolysaccharides (PubMed : 17179669, PubMed : 25912189). Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1 (PubMed : 20956340, PubMed : 25912189). Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding (Probable). Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis (PubMed : 21258343, PubMed : 28301481).
See full target information COLEC11
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Product promise

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