AB278081

Anti-COLEC11 antibody [EPR24192-169]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal COLEC11 antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

UNQ596/PRO1182, COLEC11, Collectin-11, Collectin kidney protein 1, CL-K1

3 Images

Supplier Data

Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (AB278081)

COLEC11 was immunoprecipitated from 0.35 mg Mouse plasma with ab278081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse plasma 10 μl

Lane 2 : ab278081 IP in Mouse plasma

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab278081 in Mouse plasma

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (ab278081)

Predicted band size: 29 kDa

Observed band size: 34 kDa

false

Lab

Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28772263, 30323815).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution

Lane 1:

Human plasma at 20 µL

Lane 2:

Mouse plasma at 20 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa,34 kDa

false

Lab

Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 30323815, 28772263, 20956340).

Low expression : skeletal muscle, stomach (PMID : 20956340).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 81 seconds.

All lanes:

Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human adrenal gland tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human skeletal muscle tissue lysate at 20 µg

Lane 5:

Human stomach tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa,34 kDa

false

805 Alexa Fluor® 790

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 790) preadsorbed
HRP

Goat Anti-Rabbit IgG H&L (HRP)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24192-169

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lectin that plays a role in innate immunity, apoptosis and embryogenesis (PubMed : 21258343, PubMed : 23954398, PubMed : 25912189). Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope (PubMed : 25912189). Primarily recognizes the terminal disaccharide of the glycan (PubMed : 25912189). Also recognizes a subset of fucosylated glycans and lipopolysaccharides (PubMed : 17179669, PubMed : 25912189). Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1 (PubMed : 20956340, PubMed : 25912189). Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding (Probable). Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis (PubMed : 21258343, PubMed : 28301481).

See full target information COLEC11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 14:1118889 PubMed37124627

2023

Unveiling DNA damage repair-based molecular subtypes, tumor microenvironment and pharmacogenomic landscape in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqi Kong,Zhiqiang Wang,Bingyi Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-COLEC11 antibody [EPR24192-169]

Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (AB278081)

Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081)

Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise