Rabbit Recombinant Monoclonal COLEC11 antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (AB278081), expandable thumbnail](https://content.abcam.com/products/images/colec11-antibody-epr24192-169-ab278081--immunoprecipitation-img11727.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (AB278081)")
![Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081), expandable thumbnail](https://content.abcam.com/products/images/colec11-antibody-epr24192-169-ab278081--western-blot-img9066.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081)")
![Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081), expandable thumbnail](https://content.abcam.com/products/images/colec11-antibody-epr24192-169-ab278081--western-blot-img8292.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-COLEC11 antibody [EPR24192-169] (AB278081)")
Publications
Filter by
- Frontiers in genetics 14:11188892023Unveiling DNA damage repair-based molecular subtypes, tumor microenvironment and pharmacogenomic landscape in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiqi Kong,Zhiqiang Wang,Bingyi WangPubMed 37124627
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Tested | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Lectin that plays a role in innate immunity, apoptosis and embryogenesis (PubMed:21258343, PubMed:23954398, PubMed:25912189). Calcium-dependent lectin that binds self and non-self glycoproteins presenting high mannose oligosaccharides with at least one terminal alpha-1,2-linked mannose epitope (PubMed:25912189). Primarily recognizes the terminal disaccharide of the glycan (PubMed:25912189). Also recognizes a subset of fucosylated glycans and lipopolysaccharides (PubMed:17179669, PubMed:25912189). Plays a role in innate immunity through its ability to bind non-self sugars presented by microorganisms and to activate the complement through the recruitment of MAPS1 (PubMed:20956340, PubMed:25912189). Also plays a role in apoptosis through its ability to bind in a calcium-independent manner the DNA present at the surface of apoptotic cells and to activate the complement in response to this binding (Probable). Finally, plays a role in development, probably serving as a guidance cue during the migration of neural crest cells and other cell types during embryogenesis (PubMed:21258343, PubMed:28301481).
Alternative names
UNQ596/PRO1182, COLEC11, Collectin-11, Collectin kidney protein 1, CL-K1
Recommended products
Rabbit Recombinant Monoclonal COLEC11 antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24192-169
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (ab278081), expandable thumbnail](https://content.abcam.com/products/images/colec11-antibody-epr24192-169-ab278081--immunoprecipitation-img11727.jpg "Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (ab278081)")
Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (ab278081)
COLEC11 was immunoprecipitated from 0.35 mg Mouse plasma with ab278081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse plasma 10 μl
Lane 2: ab278081 IP in Mouse plasma
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab278081 in Mouse plasma
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
All lanes: Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (ab278081)
Predicted band size: 29 kDa
Observed band size: 34 kDa
![Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081), expandable thumbnail](https://content.abcam.com/products/images/colec11-antibody-epr24192-169-ab278081--western-blot-img9066.jpg "Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081)")
Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID:30323815, 28772263, 20956340).
Low expression: skeletal muscle, stomach (PMID:20956340).
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 81 seconds.
All lanes: Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution
Lane 1: Human kidney tissue lysate at 20 µg
Lane 2: Human adrenal gland tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
Lane 4: Human skeletal muscle tissue lysate at 20 µg
Lane 5: Human stomach tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa, 34 kDa
![Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081), expandable thumbnail](https://content.abcam.com/products/images/colec11-antibody-epr24192-169-ab278081--western-blot-img8292.jpg "Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081)")
Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:28772263, 30323815).
Exposure time: 3 minutes.
All lanes: Western blot - Anti-COLEC11 antibody [EPR24192-169] (ab278081) at 1/1000 dilution
Lane 1: Human plasma at 20 µL
Lane 2: Mouse plasma at 20 µL
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa, 34 kDa
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