Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (AB278085)

This data was developed using ab278081, the same antibody clone in a different buffer formulation.

COLEC11 was immunoprecipitated from 0.35 mg Mouse plasma with ab278081 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278081 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse plasma 10 μl

Lane 2 : ab278081 IP in Mouse plasma

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab278081 in Mouse plasma

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-COLEC11 antibody [EPR24192-169] (<a href='/en-us/products/primary-antibodies/colec11-antibody-epr24192-169-ab278081'>ab278081</a>)

Predicted band size: 29 kDa

Observed band size: 34 kDa

false

• WB

Lab

Western blot - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (AB278085)

This data was developed using ab278081, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 28772263, 30323815).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-COLEC11 antibody [EPR24192-169] (<a href='/en-us/products/primary-antibodies/colec11-antibody-epr24192-169-ab278081'>ab278081</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µL

Lane 2:

Mouse plasma at 20 µL

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa,34 kDa

false

• WB

Lab

Western blot - Anti-COLEC11 antibody [EPR24192-169] - BSA and Azide free (AB278085)

This data was developed using ab278081, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 30323815, 28772263, 20956340).

Low expression : skeletal muscle, stomach (PMID : 20956340).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 81 seconds.

All lanes:

Western blot - Anti-COLEC11 antibody [EPR24192-169] (<a href='/en-us/products/primary-antibodies/colec11-antibody-epr24192-169-ab278081'>ab278081</a>) at 1/1000 dilution

Lane 1:

Human kidney tissue lysate at 20 µg

Lane 2:

Human adrenal gland tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human skeletal muscle tissue lysate at 20 µg

Lane 5:

Human stomach tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 28 kDa,34 kDa

false