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Rabbit Recombinant Monoclonal COL12 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (AB317631), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (AB317631), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (AB317631), expandable thumbnail
  • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (AB317631), expandable thumbnail
  • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (AB317631), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow CytIPICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat, Mouse

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid-beta in Alzheimer disease.

    Alternative names

    Collectin-12, Collectin placenta protein 1, Nurse cell scavenger receptor 2, Scavenger receptor class A member 4, Scavenger receptor with C-type lectin, CL-P1, hCL-P1, SRCL, COLEC12, CLP1, NSR2, SCARA4

    Recommended products

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    Rabbit Recombinant Monoclonal COL12 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Rat samples.

    Alternative names

    Collectin-12, Collectin placenta protein 1, Nurse cell scavenger receptor 2, Scavenger receptor class A member 4, Scavenger receptor with C-type lectin, CL-P1, hCL-P1, SRCL, COLEC12, CLP1, NSR2, SCARA4

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR29371-589

    Purification technique

    Affinity purification Protein A

    Specificity

    Unsuitable for rat IHC

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    ab317631 is the carrier-free version of Anti-Colec12 antibody [EPR29371-589] ab317630.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    Colec12 also known as Collectin-12 is a multifunctional protein involved in several biological processes. It is a member of the collectin family and often referred to as CL-P1 or SRCL. This protein has a mass of approximately 92 kDa and possesses a collagen-like region and a carbohydrate recognition domain. Expression of Colec12 occurs in various tissues including endothelial cells and macrophages where it shows specific affinity for certain types of sugars enabling it to interact with a range of microbial and host-derived molecules.

    Biological function summary

    This protein contributes to innate immune responses by recognizing and binding to pathogen-associated molecular patterns (PAMPs) for pathogen clearance. Colec12 partners with other proteins as part of a larger complex facilitating the opsonization of pathogens. Through this mechanism it aids in phagocytosis whereby macrophages and other immune cells effectively clear foreign particles and dead cells. Such activity maintains tissue homeostasis and protects against infection.

    Pathways

    Several molecular interactions underpin the role of Colec12 in innate immunity. Colec12 operates within the scavenger receptor pathway a critical part of pathogen detection making direct interactions with related proteins like mannose-binding lectin (MBL) which participate in complement activation. Furthermore Colec12 plays a role in endothelial function by binding to ligands involved in the coagulation cascade although it diverges slightly from classical coagulation pathways by engaging distinct receptors regulating immune responses.

    Associated diseases and disorders

    Colec12 has been implicated in conditions such as atherosclerosis and neurodegenerative diseases. Its role in clearing oxidized low-density lipoproteins (oxLDL) links it to the development of atherosclerosis as these lipids contribute to plaque formation within arteries. Additionally how Colec12 interacts with proteins like amyloid-beta suggests a potential involvement in Alzheimer's disease. Through these associations Colec12 contributes to understanding both cardiovascular and neurodegenerative pathologies.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    6 product images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using Anti-Colec12 antibody [EPR29371-589] ab317630, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Colec12 with Anti-Colec12 antibody [EPR29371-589] ab317630 at 1/500 (1.068 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Low expression: no staining on human liver.

      The section was incubated with Anti-Colec12 antibody [EPR29371-589] ab317630 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using Anti-Colec12 antibody [EPR29371-589] ab317630, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Colec12 with Anti-Colec12 antibody [EPR29371-589] ab317630 at 1/500 (1.068 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on the macrophages of human lung (PMID: 18423602)

      The section was incubated with Anti-Colec12 antibody [EPR29371-589] ab317630 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using Anti-Colec12 antibody [EPR29371-589] ab317630, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Colec12 with Anti-Colec12 antibody [EPR29371-589] ab317630 at 1/500 (1.068 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Positive staining on human placenta (PMID: 18423602)

      The section was incubated with Anti-Colec12 antibody [EPR29371-589] ab317630 for 30 mins at room temperature.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument

      Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

    • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631), expandable thumbnail

      Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using Anti-Colec12 antibody [EPR29371-589] ab317630, the same antibody clone in a different buffer formulation.

      Low expression: liver (PMID: 11564734, 16868960), pancreas (PMID:11564734).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (16868960, 11564734).

      Lanes 5-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 1-4 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      Exposure time: Lane 1: 26 seconds, lane 6: 37 seconds, lanes 2-5 and 7: 180 seconds

      All lanes: Western blot - Anti-Colec12 antibody [EPR29371-589] (Anti-Colec12 antibody [EPR29371-589] ab317630) at 1/1000 dilution

      Lane 1: Human placenta tissue lysate at 20 µg

      Lane 2: Human lung tissue lysate at 20 µg

      Lane 3: Human liver tissue lysate at 20 µg

      Lane 4: Human pancreas tissue lysate at 20 µg

      Lane 5: Rat lung tissue lysate at 20 µg

      Lane 6: Rat placenta tissue lysate at 20 µg

      Lane 7: Rat liver tissue lysate at 20 µg

      Secondary

      Lanes 1 - 4: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Lanes 5 - 7: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 110 kDa, 130 kDa, 150 kDa, 36 kDa

      Low expression: liver (PMID: 11564734, 16868960), pancreas (PMID:11564734).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (16868960, 11564734).

      Lanes 5-7 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 and lanes 1-4 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      Exposure time: Lane 1: 26 seconds, lane 6: 37 seconds, lanes 2-5 and 7: 180 seconds

    • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631), expandable thumbnail

      Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using Anti-Colec12 antibody [EPR29371-589] ab317630, the same antibody clone in a different buffer formulation.

      Low expression: A549, Jurkat (PMID:12761161).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16868960, 11564734).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-Colec12 antibody [EPR29371-589] (Anti-Colec12 antibody [EPR29371-589] ab317630) at 1/1000 dilution

      Lane 1: LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

      Lane 2: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

      Lane 3: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

      Lane 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 110 kDa, 130 kDa, 36 kDa

      Exposure time: 180s

      Low expression: A549, Jurkat (PMID:12761161).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16868960, 11564734).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    • Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631), expandable thumbnail

      Western blot - Anti-Colec12 antibody [EPR29371-589] - BSA and Azide free (ab317631)

      This data was developed using Anti-Colec12 antibody [EPR29371-589] ab317630, the same antibody clone in a different buffer formulation.

      Collectin-12 is a glycoprotein of approximately 110-150 kDa and detected as a 90-kDa band after treated with Peptide:N-glycosidase F (PNGase F).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16868960, 11564734).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-Colec12 antibody [EPR29371-589] (Anti-Colec12 antibody [EPR29371-589] ab317630) at 1/1000 dilution

      Lane 1: Untreated 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

      Lane 2: 293T cell lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Observed band size: 110-150 kDa, 90 kDa, 36 kDa

      Exposure time: 180s

      Collectin-12 is a glycoprotein of approximately 110-150 kDa and detected as a 90-kDa band after treated with Peptide:N-glycosidase F (PNGase F).

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16868960, 11564734).

      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

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