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Mouse Monoclonal COL1A1 antibody. Carrier free. Suitable for IHC-P, ELISA, WB and reacts with Mouse, Human, Recombinant fragment - Human samples. Cited in 75 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1000-1150.
Alternative names=Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
- Isotype
IgG3
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Fragment Protein within Human COL1A1 aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information. Database link: P02452
Reactivity data
Select an application| IHC-P | ELISA | WB | |
|---|---|---|---|
| Human | Tested | Expected | Expected |
| Mouse | Tested | Expected | Tested |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended |
Images
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal COL1A1 antibody. Carrier free. Suitable for IHC-P, ELISA, WB and reacts with Mouse, Human, Recombinant fragment - Human samples. Cited in 75 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1000-1150.
Alternative names=Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Key facts
- Isotype
IgG3
- Host species
Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
Recombinant Fragment Protein within Human COL1A1 aa 1000-1150. The exact immunogen used to generate this antibody is proprietary information. Database link: P02452
- Carrier free
Yes
- Clone number
3G3
- Purity
Tissue culture supernatant
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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