AB21286

Anti-Collagen I antibody

Name: Anti-cola1 antibody - rabbit polyclonal (ab21286) | Abcam
Brand: Abcam Limited
SKU: AB21286
Price: 400 USD
Availability: InStock
Rating: 4 (16 reviews)

Lab Essentials
What is this?

(16 Reviews)

(292 Publications)

Anti-Collagen I antibody (ab21286) is a rabbit polyclonal antibody detecting Collagen I in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Mouse.

- Over 230 publications
- Trusted since 2005

View Alternative Names

Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

ELISA, WB, ICC/IF, RIA

applications

Immunogen

Native Full Length Protein corresponding to Mouse Col1a1.

P11087

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Reactivity data

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Product details

Anti-Collagen I antibody (ab21286) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, RIA, WB in mouse samples.

Anti-Collagen I antibody (ab21286) has been cited over 230 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Collagen I antibody (ab21286) has high sensitivity and specificity.

Anti-Collagen I (Cola1; Collagen type I) antibody (ab21286) has 16 independent reviews from customers.

Anti-Collagen I antibody (ab21286) specifically detects Collagen I (UniProt ID: P02452; Molecular weight: ~140kDa) and is sold in 100 µL selling sizes.

Collagen I, the most abundant collagen type in the human body, plays a significant role in oncology. It is a major component of the extracellular matrix (ECM) and contributes to the structural integrity of tissues. In cancer, Collagen I can influence tumor progression by promoting tumor cell proliferation, invasion, and metastasis through interactions with various cell surface receptors and signaling pathways.

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Properties and storage information

Purification notes

Purified by Ion exchange chromatography (DEAE-Trisacryl).

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information Col1a1

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Publications (292)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed40985890

2025

Nf2/FGFR1/AKT axis directs cranial neural crest-derived skull morphogenesis via collagen synthesis and trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping Huang,Junguang Liao,Panpan Shen,Yiliang He,Fuju Sun,Qi Zhang,Changlin Zheng,Xingen Zhang,Haibo Li,Guiqian Chen

Science advances 11:eadv2628 PubMed40668913

2025

Multicellular muscle-tendon bioprinting of mechanically optimized musculoskeletal bioactuators with enhanced force transmission.

Applications

Unspecified application

Species

Unspecified reactive species

Miriam Filippi,Diana Mock,Judith Fuentes,Mike Y Michelis,Aiste Balciunaite,Pablo Paniagua,Raoul Hopf,Adina Barteld,Selina Eng,Asia Badolato,Jess Snedeker,Maria Guix,Samuel Sanchez,Robert K Katzschmann

Cell death & disease 16:505 PubMed40634289

2025

Failure to resolve inflammation contributes to juvenile onset cardiac damage in a mouse model of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

James S Novak,Amy Lischin,Prech Uapinyoying,Ravi Hindupur,Young Jae Moon,Surajit Bhattacharya,Sarah Tiufekchiev-Grieco,Victoria Barone,Davi A G Mázala,Iteoluwakishi H Gamu,Gabriela Walters,Jyoti K Jaiswal

The Journal of clinical investigation 135: PubMed40540411

2025

Angiopoietin-like protein 2 mediates vasculopathy-driven fibrogenesis in a mouse model of systemic sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dyuti Saha,Ravi Kiran Annadorai,Sujaya Thannimangalath,Neha P Shroff,Sunny Kataria,Binita Dam,Abhik Dutta,Akshay Hegde,Ankita Hiwale,Venkatesh Ravula,Shagnik Saha,Lekshmi Minikumari Rahulan,Neha Nigam,Neha Singh,Vikas Agarwal,Praveen K Vemula,Colin Jamora

Calcified tissue international 116:81 PubMed40478277

2025

Mineral Content and Extracellular Matrix Protein Expression in Mouse Growth Plates During Epiphyseal Fusion: An Observational Study.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhang Yu,Megumi Nakamura,Miyuki Mayanagi,Itaru Mizoguchi,Yasuyuki Sasano

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70666 PubMed40471098

2025

METTL3 Modulates Radiation-Induced Cardiac Fibrosis via the Akt/mTOR Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsong Qiao,Chao Tang,Jingjing Zhu,Yu Feng,Li Xiang,Jing Zhu,Xiaosong Gu

Micromachines 16: PubMed40428678

2025

Low-Temperature Electrospinning-Fabricated Three-Dimensional Nanofiber Scaffolds for Skin Substitutes.

Applications

Unspecified application

Species

Unspecified reactive species

Qiqi Dai,Huazhen Liu,Wenbin Sun,Yi Zhang,Weihuang Cai,Chunxiang Lu,Kaidi Luo,Yuanyuan Liu,Yeping Wang

Cells 14: PubMed40422230

2025

Taurine Prevents Impairments in Skin Barrier Function and Dermal Collagen Synthesis Triggered by Sleep Deprivation-Induced Estrogen Circadian Rhythm Disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Shao,Zhaoyang Wang,Yifang Li,Xun Tang,Ziyi Li,Huan Xia,Qihong Wu,Ruxue Chang,Chunna Wu,Tao Meng,Yufei Fan,Yadong Huang,Yan Yang

International journal of molecular sciences 26: PubMed40243849

2025

Inhibiting Myostatin Expression by the Antisense Oligonucleotides Improves Muscle Wasting in a Chronic Kidney Disease Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Arju Akhter,Abdullah Md Sheikh,Jun Yoshino,Takeshi Kanda,Atsushi Nagai,Masafumi Matsuo,Shozo Yano

Theranostics 15:4526-4549 PubMed40225577

2025

Targeting USP11 counteracts -associated interstitial lung disease in hiPSCs-derived alveolar organoids and in vivo models.

Applications

Unspecified application

Species

Unspecified reactive species

Janardhan Keshav Karapurkar,Sripriya Rajkumar,Ji-Hye Jung,Ji-Young Kim,Girish Birappa,D A Ayush Gowda,Jencia Carminha Colaco,Bharathi Suresh,Jung-Yun Choi,Sang Hyeon Woo,Won-Jun Jo,Jong-Hee Lee,Kye-Seong Kim,Seok-Ho Hong,Suresh Ramakrishna

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