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AB233080

Anti-Collagen I antibody

1

(1 Review)

|

(18 Publications)

Rabbit Polyclonal COL1A1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Pig samples. Cited in 18 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1200-1450.

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

2 Images
Western blot - Anti-Collagen I antibody (AB233080)
  • WB

Supplier Data

Western blot - Anti-Collagen I antibody (AB233080)

All lanes:

Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL

All lanes:

Recombinant human Collagen I protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 139 kDa

false

Western blot - Anti-Collagen I antibody (AB233080)
  • WB

Supplier Data

Western blot - Anti-Collagen I antibody (AB233080)

All lanes:

Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL

Lane 1:

Human placenta lysate

Lane 2:

Human liver lysate

Lane 3:

Pig skin lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 139 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human COL1A1 aa 1200-1450. The exact immunogen used to generate this antibody is proprietary information.

P02452

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Positive Control: Hu stomach, skin and adrenal gland tissue lysates.</p><p>Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Positive Control: Hu stomach, skin and adrenal gland tissue lysates.</p><p>Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.</p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Positive Control: Hu stomach, skin and adrenal gland tissue lysates.</p><p>Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab233080 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information COL1A1

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Tissue engineering and regenerative medicine 22:57-75 PubMed39579168

2024

Development of Zinc-Containing Chitosan/Gelatin Coatings with Immunomodulatory Effect for Soft Tissue Sealing around Dental Implants.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Han,Jorine G F Sanders,Lea Andrée,Bart A J A van Oirschot,Adelina S Plachokova,Jeroen J J P van den Beucken,Sander C G Leeuwenburgh,Fang Yang

Lasers in surgery and medicine 56:298-304 PubMed38304960

2024

Histological examination of skin tissue in the porcine animal model after application of a new monopolar radiofrequency.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Meng Wang,Yi Li,Yan Yan

The Journal of biological chemistry 300:105502 PubMed38016515

2023

Fatty acids and inflammatory stimuli induce expression of long-chain acyl-CoA synthetase 1 to promote lipid remodeling in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Hong Wang, Surbhi,Sayhaan Goraya,Jaeman Byun,Subramaniam Pennathur

Stem cells translational medicine 12:838-848 PubMed37804518

2023

Autologous Airway Basal Cell Transplantation Alleviates Airway Epithelium Defect in Recurrent Benign Tracheal Stenosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong-Shun Ye,Di-Fei Chen,Ming Liu,Yu-Long Luo,Huan-Jie Chen,Hai-Kang Zeng,Jing-Wei Liu,Yi-Ping Zhu,Xin-Yu Song,Chang-Qin Lin,Zhu-Quan Su,Shi-Yue Li

iScience 26:107618 PubMed37664614

2023

Thioredoxin-1 promotes the restoration of alveolar bone in periodontitis with diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyan Wu,Yaxian Huang,Chi Zhan,Lingling Chen,Zhengmei Lin,Zhi Song

Advanced healthcare materials 12:e2301180 PubMed37463568

2023

Selective Extracellular Matrix Guided Mesenchymal Stem Cell Self-Aggregate Engineering for Replication of Meniscal Zonal Tissue Gradient in a Porcine Meniscectomy Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sujin Noh,Yong Jun Jin,Dong Il Shin,Hyeon Jae Kwon,Hee-Woong Yun,Kyu Min Kim,Jae-Young Park,Jun Young Chung,Do Young Park

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 250:152141 PubMed37499701

2023

Postnatal morpho-functional development of a dog's meniscus.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Clotilde Modina,Lucia Aidos,Valentina Rafaela Herrera Millar,Margherita Pallaoro,Umberto Polito,Maria Cristina Veronesi,Giuseppe Maria Peretti,Laura Mangiavini,Liliana Carnevale,Federica Boschetti,Francesco Abbate,Alessia Di Giancamillo

Bioengineering (Basel, Switzerland) 10: PubMed36978682

2023

Long-Bone-Regeneration Process in a Sheep Animal Model, Using Hydroxyapatite Ceramics Prepared by Tape-Casting Method.

Applications

Unspecified application

Species

Unspecified reactive species

Lenka Kresakova,Lubomir Medvecky,Katarina Vdoviakova,Maros Varga,Ján Danko,Roman Totkovic,Tatiana Spakovska,Marko Vrzgula,Maria Giretova,Jaroslav Briancin,Veronika Šimaiová,Marian Kadasi

Biomedicines 11: PubMed36979856

2023

Regenerative Potential of Hydroxyapatite-Based Ceramic Biomaterial on Mandibular Cortical Bone: An Study.

Applications

Unspecified application

Species

Unspecified reactive species

Katarína Vdoviaková,Andrej Jenca,Andrej Jenca,Ján Danko,Lenka Kresáková,Veronika Simaiová,Peter Reichel,Pavol Rusnák,Jozef Pribula,Marko Vrzgula,Sarah J Askin,Maria Giretová,Jaroslav Briancin,Lubomír Medvecký

Bio-medical materials and engineering 34:289-304 PubMed36617774

2023

Reduced fibrous capsule elastic fibers from biologic ECM-enveloped CIEDs in minipigs, supported with a novel compression mechanics model.

Applications

Unspecified application

Species

Unspecified reactive species

Roche C de Guzman,Allison S Meer,Aidan A Mathews,Atara R Israel,Michael T Moses,Clarence M Sams,Daniel B Deegan
View all publications

Product promise

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