Rabbit Polyclonal COL1A1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Pig samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1200-1450.
IgG
Rabbit
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Chicken | Predicted |
Cow | Predicted |
Dog | Predicted |
Pig | Expected |
Recombinant fragment - Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 1-5 µg/mL | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
Species Human | Dilution info 1-5 µg/mL | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
Species | Dilution info | Notes |
---|---|---|
Species Pig | Dilution info 1-5 µg/mL | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Dog | Dilution info - | Notes - |
Select an associated product type
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Rabbit Polyclonal COL1A1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Pig samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1200-1450.
IgG
Rabbit
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Liquid
Polyclonal
Affinity purification Immunogen
ab233080 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
All lanes: Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL
Lane 1: Human placenta lysate
Lane 2: Human liver lysate
Lane 3: Pig skin lysate
All lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 139 kDa
All lanes: Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL
All lanes: Recombinant human Collagen I protein
All lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 139 kDa
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