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Rabbit Polyclonal COL1A1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Pig samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1200-1450.


Images

Western blot - Anti-Collagen I antibody (AB233080), expandable thumbnail
  • Western blot - Anti-Collagen I antibody (AB233080), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human COL1A1 aa 1200-1450. The exact immunogen used to generate this antibody is proprietary information. Database link P02452

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Chicken
Predicted
Cow
Predicted
Dog
Predicted
Pig
Expected
Recombinant fragment - Human
Tested

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1-5 µg/mL

Notes

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

Species

Human

Dilution info

1-5 µg/mL

Notes

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

Expected
Expected

Species

Pig

Dilution info

1-5 µg/mL

Notes

Positive Control: Hu stomach, skin and adrenal gland tissue lysates.

Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.

Predicted
Predicted

Species

Mouse, Rat, Chicken, Cow, Dog

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Alternative names

Recommended products

Rabbit Polyclonal COL1A1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Pig samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1200-1450.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human COL1A1 aa 1200-1450. The exact immunogen used to generate this antibody is proprietary information. Database link P02452
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab233080 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Associated diseases and disorders

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - Anti-Collagen I antibody (ab233080), expandable thumbnail

    Western blot - Anti-Collagen I antibody (ab233080)

    All lanes: Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL

    Lane 1: Human placenta lysate

    Lane 2: Human liver lysate

    Lane 3: Pig skin lysate

    Secondary

    All lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Predicted band size: 139 kDa

  • Western blot - Anti-Collagen I antibody (ab233080), expandable thumbnail

    Western blot - Anti-Collagen I antibody (ab233080)

    All lanes: Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL

    All lanes: Recombinant human Collagen I protein

    Secondary

    All lanes: HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

    Predicted band size: 139 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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