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AB233080

Anti-Collagen I antibody

1

(1 Review)

|

(9 Publications)

Rabbit Polyclonal COL1A1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human, Pig samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1 aa 1200-1450.

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

2 Images
Western blot - Anti-Collagen I antibody (AB233080)
  • WB

Supplier Data

Western blot - Anti-Collagen I antibody (AB233080)

All lanes:

Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL

All lanes:

Recombinant human Collagen I protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 139 kDa

false

Western blot - Anti-Collagen I antibody (AB233080)
  • WB

Supplier Data

Western blot - Anti-Collagen I antibody (AB233080)

All lanes:

Western blot - Anti-Collagen I antibody (ab233080) at 2 µg/mL

Lane 1:

Human placenta lysate

Lane 2:

Human liver lysate

Lane 3:

Pig skin lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 139 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human COL1A1 aa 1200-1450. The exact immunogen used to generate this antibody is proprietary information.

P02452

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Positive Control: Hu stomach, skin and adrenal gland tissue lysates.</p><p>Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Positive Control: Hu stomach, skin and adrenal gland tissue lysates.</p><p>Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.</p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p>Positive Control: Hu stomach, skin and adrenal gland tissue lysates.</p><p>Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab233080 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information COL1A1

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107618 PubMed37664614

2023

Thioredoxin-1 promotes the restoration of alveolar bone in periodontitis with diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyan Wu,Yaxian Huang,Chi Zhan,Lingling Chen,Zhengmei Lin,Zhi Song

Advanced healthcare materials 12:e2301180 PubMed37463568

2023

Selective Extracellular Matrix Guided Mesenchymal Stem Cell Self-Aggregate Engineering for Replication of Meniscal Zonal Tissue Gradient in a Porcine Meniscectomy Model.

Applications

Unspecified application

Species

Unspecified reactive species

Sujin Noh,Yong Jun Jin,Dong Il Shin,Hyeon Jae Kwon,Hee-Woong Yun,Kyu Min Kim,Jae-Young Park,Jun Young Chung,Do Young Park

Bio-medical materials and engineering 34:289-304 PubMed36617774

2023

Reduced fibrous capsule elastic fibers from biologic ECM-enveloped CIEDs in minipigs, supported with a novel compression mechanics model.

Applications

Unspecified application

Species

Unspecified reactive species

Roche C de Guzman,Allison S Meer,Aidan A Mathews,Atara R Israel,Michael T Moses,Clarence M Sams,Daniel B Deegan

Pharmaceuticals (Basel, Switzerland) 15: PubMed36422523

2022

Three-Dimensional Microtumor Formation of Infantile Hemangioma-Derived Endothelial Cells for Mechanistic Exploration and Drug Screening.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Li,Xinglong Zhu,Meng Kong,Siyuan Chen,Ji Bao,Yi Ji

BMC complementary medicine and therapies 22:63 PubMed35272637

2022

Icariin regulates stem cell migration for endogenous repair of intervertebral disc degeneration by increasing the expression of chemotactic cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaofei Zhang,Fengwei Qin,Yonghui Feng,Sineng Zhang,Chunliang Xie,He Huang,Chaohui Sang,Shaoyu Hu,Feng Jiao,Jie Jiang,Yi Qin

Materials today. Bio 14:100234 PubMed35308043

2022

Repair of osteochondral defects mediated by double-layer scaffolds with natural osteochondral-biomimetic microenvironment and interface.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Wei Xu,Xintong Zhao,Baoshuai Bai,Yuejie Hua,Jincheng Tang,Feifan Chen,Yu Liu,Yahui Wang,Guangdong Zhou,Yilin Cao

Cell death & disease 12:474 PubMed33980818

2021

Fas/FasL mediates NF-κBp65/PUMA-modulated hepatocytes apoptosis via autophagy to drive liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Siwei Tan,Xianzhi Liu,Lingjun Chen,Xiaoqin Wu,Li Tao,Xuemei Pan,Shuyan Tan,Huiling Liu,Jie Jiang,Bin Wu

American journal of translational research 12:6464-6477 PubMed33194044

2020

Erythropoietin enhances meniscal regeneration and prevents osteoarthritis formation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Ning Fu,Hui-Wu Li,Na Du,Xu Liang,Shi-Hao He,Kuang-Jin Guo,Tian-Fang Li

Frontiers in cardiovascular medicine 6:170 PubMed31921893

2019

Differences in microRNA-29 and Pro-fibrotic Gene Expression in Mouse and Human Hypertrophic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yamin Liu,Junaid Afzal,Styliani Vakrou,Gabriela V Greenland,C Conover Talbot,Virginia B Hebl,Yufan Guan,Rehan Karmali,Jil C Tardiff,Leslie A Leinwand,Jeffrey E Olgin,Samarjit Das,Ryuya Fukunaga,M Roselle Abraham
View all publications

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