Rabbit Polyclonal COL1A1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human COL1A1.
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- Disease models & mechanisms 16:2023Lack of expression of miR-29a/b1 impairs bladder function in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZunyi Wang,Robert Spitz,Chad Vezina,Jianghui Hou,Dale E BjorlingPubMed 37283037
- Aesthetic plastic surgery 47:2093-21052023Breast Implant Capsule: A Murine Model Comparing Capsular Contracture Susceptibility Among Six Breast Implants Available in the Market.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Bérniz,Francisco Carmona-Torre,Cristina Gómez-Martínez,Leire Fernéndez-Ciriza,Jose Luis Del Pozo,Bernardo HontanillaPubMed 37022396
- The European respiratory journal 61:2023Eosinophils protect against pulmonary hypertension through 14-HDHA and 17-HDHA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Shu,Jiawei Zhang,Yitian Zhou,Zhihua Chen,Jinqiu Li,Qihao Tang,Wenqi Lei,Yanjiang Xing,Jing Wang,Chen WangPubMed 36423907
- BMC cardiovascular disorders 22:4922022Elucidating the role of circNFIB in myocardial fibrosis alleviation by endogenous sulfur dioxide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Liu,Ranran Zhang,Dahai Wang,Yi Lin,Cui Bai,Nana Nie,Shan Gao,Qiuye Zhang,Hong Chang,Chongmin RenPubMed 36404310
- International journal of molecular sciences 23:2022Intestinal Radiation Protection and Mitigation by Second-Generation Probiotic Engineered to Deliver Interleukin-22.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexis Espinal,Michael W Epperly,Amitava Mukherjee,Renee Fisher,Donna Shields,Hong Wang,M Saiful Huq,Diala Fatima Hamade,Anda M Vlad,Lan Coffman,Ronald Buckanovich,Jian Yu,Brian J Leibowitz,Jan-Peter van Pijkeren,Ravi B Patel,Donna Stolz,Simon Watkins,Asim Ejaz,Joel S GreenbergerPubMed 35628427
- Signal transduction and targeted therapy 7:1572022Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingyao Wang,Zhe Zhang,Jiangfeng Liu,Meiyue Song,Tiantian Zhang,Yiling Chen,Huiyuan Hu,Peiran Yang,Bolun Li,Xiaomin Song,Junling Pang,Yanjiang Xing,Zhujie Cao,Wenjun Guo,Hao Yang,Jing Wang,Juntao Yang,Chen WangPubMed 35551173
- eLife 11:2022Multi-omic rejuvenation of human cells by maturation phase transient reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiljeet Gill,Aled Parry,Fátima Santos,Hanneke Okkenhaug,Christopher D Todd,Irene Hernando-Herraez,Thomas M Stubbs,Inês Milagre,Wolf ReikPubMed 35390271
- Bioscience reports 42:2022High glucose represses the proliferation of tendon fibroblasts by inhibiting autophagy activation in tendon injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFu-Chen Song,Jia-Qin Yuan,Mei-Dong Zhu,Qi Li,Sheng-Hua Liu,Lei Zhang,Cheng ZhaoPubMed 35293974
- PloS one 17:e02631412022Bio-fabrication of stem-cell-incorporated corneal epithelial and stromal equivalents from silk fibroin and gelatin-based biomaterial for canine corneal regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChutirat Torsahakul,Nipan Israsena,Supaporn Khramchantuk,Juthamas Ratanavaraporn,Sirakarnt Dhitavat,Watchareewan Rodprasert,Sirirat Nantavisai,Chenphop SawangmakePubMed 35120168
- Orthopaedic surgery 13:2457-24672021Characterization of a Novel Model of Lumbar Ligamentum Flavum Hypertrophy in Bipedal Standing Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen-Yu Zheng,Peng Li,Xiang Ao,Lei Qian,Yong-Xing Peng,Jun Chu,Tao Jiang,Zheng-Nan Lian,Zhong-Min Zhang,Liang WangPubMed 34651434
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human COL1A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02452
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Expected | Tested |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Incubate slides for 20 minutes at 90° C in 10 mM sodium citrate (pH 6.0) / 0.1% Tween-20). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Recommended products
Rabbit Polyclonal COL1A1 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Cited in 22 publications. Immunogen corresponding to Synthetic Peptide within Human COL1A1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human COL1A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02452
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
- Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
- Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
- Associated diseases and disorders
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody (ab254113)
Immunohistochemical analysis of formaldehyde-fixed fibrotic mouse lung tissue staining collagen I with ab254113 (red). Positive staining of collagen I that has formed fibrils in the extracellular matrix.
Western blot - Anti-Collagen I antibody (ab254113)
All lanes: Western blot - Anti-Collagen I antibody (ab254113) at 1/1000 dilution
All lanes: Rat lung lysate at 15 µg
Predicted band size: 139 kDa
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