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Rabbit Polyclonal COL1A1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1859 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL1A1.
Alternative names=Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
Native Full Length Protein corresponding to Human COL1A1. Database link: P02452
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Not recommended | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Rabbit Polyclonal COL1A1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1859 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human COL1A1.
Alternative names=Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
Native Full Length Protein corresponding to Human COL1A1. Database link: P02452
Affinity purification Immunogen
This product is not recommended for use under denaturing conditions in WB. We would suggest testing it under native conditions. Denaturing and reducing conditions will greatly diminish reactivity and selectivity of this antibody. Abcam does not test ab34710 with endogenous samples in WB. We do recommend to look at the guidelines for blotting large proteins.
ab34710 has <5% cross-reactivity with Collagen III
Customers have been successful using ab34710 in this application, please see reference Tillgren V et al.J BiolChem290:918-25; 2015.
Positive Control: Human stomach, skin and adrenal gland tissue lysates.
COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Anti-Collagen I antibody (ab34710) is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.
This collagen antibody was developed using non-denatured 3D epitopes, you must be careful not to denature the collagen protein during your experiment.
PLEASE READ THESE IMPORTANT PROTOCOL TIPS, clickhere for the english version or here for the mandarin version.
It is often extremely difficult to generate antibodies with specificities to collagens due to the uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes - this may result in diminished reactivity of some antibodies with denatured collagen or formalin-fixed, paraffin embedded tissues.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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