AB255809

Anti-Collagen I antibody [EPR22209-75]

RabMAb
Recombinant
Lab Essentials
20ul selling size
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(21 Publications)

Rabbit Recombinant Monoclonal COL1A1 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 21 publications.

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

3 Images

Lab

Western blot - Anti-Collagen I antibody [EPR22209-75] (AB255809)

Procollagen I (220 kDa) and cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 23940311).

Exposure time : 3 minutes.

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809) at 1/1000 dilution

Lane 1:

HFF-1 (human skin fibroblast) whole cell lysate at 20 µg

Lane 2:

HFF-1 culture supernatant at 10 µL

Lane 3:

MRC-5 (human lung fibroblast) whole cell lysate at 20 µg

Lane 4:

MRC-5 culture supernatant at 10 µL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 220 kDa,35 kDa

false

Lab

Western blot - Anti-Collagen I antibody [EPR22209-75] (AB255809)

Blocking/Diluting buffer and concentration 5% NFDM/TBST. Exposure time : Lane1-7： 180 seconds Lane 8： 60 seconds We recommend loading higher amount of lysate or using lower antibody dilution.

Lanes 1 - 8:

Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809) at 1/1000 dilution

Lanes 1 - 8:

Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22209-75-bsa-and-azide-free-ab256541'>ab256541</a>)

Lane 1:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

MDA-MB-231(Human breast adenocarcinoma epithelial cell) supernatant lysate at 20 µg

Lane 4:

A431(Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Human lung tissue lysate at 20 µg

Lane 7:

Human liver tissue lysate at 20 µg

Lane 8:

HFF-1(Human Skin fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Lab

Western blot - Anti-Collagen I antibody [EPR22209-75] (AB255809)

Cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 23940311).

Exposure time : 26 seconds.

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Collagen I antibody [EPR22209-75] (ab255809) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) culture supernatant 10 ul

Lane 2:

Mouse kidney organ culture supernatant 10 ul

Lane 3:

Mouse lung organ culture supernatant 10 ul

Lane 4:

Rat lung organ culture supernatant 10 ul

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 139 kDa

Observed band size: 35 kDa

false

Carrier free

Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22209-75

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Positive Control: Hu stomach, skin and adrenal gland tissue lysates.Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Positive Control: Hu stomach, skin and adrenal gland tissue lysates.Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Positive Control: Hu stomach, skin and adrenal gland tissue lysates.Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information COL1A1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 13:e70553 PubMed40969143

2025

Midkine promotes PDGF-BB-induced proliferation, migration, and glycolysis of airway smooth muscle cells via the PI3K Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Xu,Wenli Li,Jun Shi,Yanfang Guo

Proceedings of the National Academy of Sciences of the United States of America 122:e2514837122 PubMed40811463

2025

Stress granule-mediated ZBP1 activation drives necroptotic cell death in non-obstructive azoospermia and testicular aging.

Applications

Unspecified application

Species

Unspecified reactive species

Hongen Lei,Dianrong Li,Jie Chen,Baowen Du,Kaiju Jiang,Tao Xu,Hu Han,Weiliang Fan,Long Tian,Xiaodong Wang

Stem cell research & therapy 15:349 PubMed39380096

2024

SLAMF8 regulates osteogenesis and adipogenesis of bone marrow mesenchymal stem cells via S100A6/Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yibo Wang,Kai Hang,Xiaoyong Wu,Li Ying,Zhongxiang Wang,Zemin Ling,Hao Hu,Zhijun Pan,Xuenong Zou

iScience 27:110801 PubMed39310777

2024

Modifying MSCs-derived EVs with esterase-responsive and charge-reversal cationic polymers enhances bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yihan Chen,Bang Li,Mukeshimana Christelle,Nshimiyimana Eugene,Wenjia Han,Hong Zhou,Nasha Qiu,Hengguo Zhang,Jianguang Xu

Bioactive materials 37:222-238 PubMed38549772

2024

ETV2 regulating PHD2-HIF-1α axis controls metabolism reprogramming promotes vascularized bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

HaoRan Du,Bang Li,Rui Yu,Xiaoxuan Lu,ChengLin Li,HuiHui Zhang,Fan Yang,RongQuan Zhao,WeiMin Bao,Xuan Yin,YuanYin Wang,Jian Zhou,Jianguang Xu

International journal of biological sciences 20:585-605 PubMed38169591

2024

SIRT5-related lysine demalonylation of GSTP1 contributes to cardiomyocyte pyroptosis suppression in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Can Wei,Meixin Shi,Shiyun Dong,Zhitao Li,Bingbing Zhao,Dan Liu,Guopeng Li,Jie Cen,Ligen Yu,Xiao Liang,Lili Shi

Current issues in molecular biology 45:10159-10178 PubMed38132480

2023

Improvement in Yield of Extracellular Vesicles Derived from Edelweiss Callus Treated with LED Light and Enhancement of Skin Anti-Aging Indicators.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Jung Kim,Hoon Ko,Ji-Young Kim,Hye-Jin Kim,Hwi-Yeob Kim,Hang-Eui Cho,Hyun-Dae Cho,Won-Sang Seo,Hee-Cheol Kang

Iranian journal of basic medical sciences 26:717-724 PubMed37275755

2023

Irisin attenuates angiotensin II-induced atrial fibrillation and atrial fibrosis via LOXL2 and TGFβ1/Smad2/3 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yingbiao Wu,Jun Luo,Xiang Song,Wei Gu,Saihua Wang,Shuwen Hao,Zhiwu Dong,Zhongping Ning

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22877 PubMed37014317

2023

Circular RNA PDK1 targets miR-4731-5p to enhance TNXB expression in ligamentum flavum hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xin Wang,Li-Ting Zeng,Xiao Yang,Xiao-Fei Cheng,Hai-Jun Tian,Chen Chen,Xiao-Jiang Sun,Chang-Qing Zhao,Hui Ma,Jie Zhao

Cells 11: PubMed36552853

2022

Icariin Treatment Rescues Diabetes Induced Bone Loss via Scavenging ROS and Activating Primary Cilia/Gli2/Osteocalcin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Qingfeng Cheng,Xiangmei Wu,Huifang Zhu,Xiaoyan Deng,Maorong Wang,Shengyong Yang,Jie Xu,Qian Chen,Mengxue Li,Xianjun Liu,Changdong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen I antibody [EPR22209-75]

Western blot - Anti-Collagen I antibody [EPR22209-75] (AB255809)

Western blot - Anti-Collagen I antibody [EPR22209-75] (AB255809)

Western blot - Anti-Collagen I antibody [EPR22209-75] (AB255809)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise