Rabbit Recombinant Monoclonal COL1A1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (AB256541), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-epr22209-75-bsa-and-azide-free-ab256541--western-blot-img18214.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (AB256541)")
![Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (AB256541), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-epr22209-75-bsa-and-azide-free-ab256541--western-blot-img417132.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (AB256541)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Expected | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
Species Rat | Dilution info - | Notes Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Recommended products
Rabbit Recombinant Monoclonal COL1A1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22209-75
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab256541 is the carrier-free version of Anti-Collagen I antibody [EPR22209-75] ab255809.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
- Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
- Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
- Associated diseases and disorders
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-epr22209-75-bsa-and-azide-free-ab256541--western-blot-img18214.jpg "Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)")
Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)
Procollagen I (220 kDa) and cleaved C-propeptide (35 kDa) are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).
Exposure time: 3 minutes.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Collagen I antibody [EPR22209-75] ab255809).
All lanes: Western blot - Anti-Collagen I antibody [EPR22209-75] (Anti-Collagen I antibody [EPR22209-75] ab255809) at 1/1000 dilution
Lane 1: HFF-1 (human skin fibroblast) whole cell lysate at 20 µg
Lane 2: HFF-1 culture supernatant at 10 µL
Lane 3: MRC-5 (human lung fibroblast) whole cell lysate at 20 µg
Lane 4: MRC-5 culture supernatant at 10 µL
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 139 kDa
Observed band size: 220 kDa, 35 kDa
![Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-epr22209-75-bsa-and-azide-free-ab256541--western-blot-img417132.jpg "Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)")
Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)
Blocking/Diluting buffer and concentration 5% NFDM/TBST.
Exposure time:
Lane1-7： 180 seconds
Lane 8： 60 seconds
We recommend loading higher amount of lysate or using lower antibody dilution.Lanes 1 - 8: Western blot - Anti-Collagen I antibody [EPR22209-75] (Anti-Collagen I antibody [EPR22209-75] ab255809) at 1/1000 dilution
Lanes 1 - 8: Western blot - Anti-Collagen I antibody [EPR22209-75] - BSA and Azide free (ab256541)
Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: MDA-MB-231(Human breast adenocarcinoma epithelial cell) supernatant lysate at 20 µg
Lane 4: A431(Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Human lung tissue lysate at 20 µg
Lane 7: Human liver tissue lysate at 20 µg
Lane 8: HFF-1(Human Skin fibroblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com