Anti-collagen I antibody [EPR22894-89] is a rabbit monoclonal antibody that is used in collagen alpha 1(I) chain western blot (WB) and immunoprecipitation (IP). Suitable for human, mouse and rat samples.
- Cited in 105 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR22894-89 is cited in over 170 publications
- Affinity purified using Protein A
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Expected | Tested | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
Rat | Expected | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting. |
Species Rat | Dilution info 1/1000 | Notes Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting. |
Species Human | Dilution info 1/1000 | Notes Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Select an associated product type
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Anti-collagen I antibody [EPR22894-89] is a rabbit monoclonal antibody that is used in collagen alpha 1(I) chain western blot (WB) and immunoprecipitation (IP). Suitable for human, mouse and rat samples.
- Cited in 105 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR22894-89 is cited in over 170 publications
- Affinity purified using Protein A
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR22894-89
Affinity purification Protein A
Compared with ab260043, ab255809 has lower background. We recommend ab255809 as an alternative for western blotting.
Please navigate to the support & downloads section for specificity details in Chinese.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Collagen I was immunoprecipitated from 0.35 mg Mouse lung lysate 10ug with ab260043 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260043 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution. Lane 1: Mouse lung lysate 10ug
Lane 2: ab260043 IP in Mouse lung lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab260043 in Mouse lung lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).
All lanes: Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (ab260043)
Predicted band size: 139 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Procollagen I (220 kDa) and cleaved C-propeptide (35 kDa) are observed.
The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).
Exposure time: Lane 1: 1 second; Lane 2: 3 seconds.
All lanes: Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/5000 dilution
Lane 1: HFF-1 (human skin fibroblast) whole cell lysate at 20 µg
Lane 2: HFF-1 culture supernatant at 10 µL
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/25000 dilution
Predicted band size: 139 kDa
Observed band size: 220 kDa, 35 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Cleaved collagen I C-propeptide (35 kDa) is observed.
The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).
Exposure time: Lanes 1-2: 3 seconds; Lane 3: 136 seconds.
All lanes: Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) culture supernatant at 10 µL
Lane 2: Rat lung organ culture supernatant at 10 µL
Lane 3: Human lung tissue lysate at 10 µg
Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/25000 dilution
Lane 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 139 kDa
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HFF-1 (Human skin fibroblast) cells labelling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Exposure Time: ab260043: 20 seconds, Anti-Collagen I antibody [EPR22209-75] ab255809: 180 seconds.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as loading control.
Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting.
For better use Anti-Collagen I antibody [EPR22209-75] ab255809, we recommend loading higher amount of lysate or using lower antibody dilution.
All lanes: Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/1000 dilution
Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3: MDA-MB-231(Human breast adenocarcinoma epithelial cell) supernatant lysate
Lane 4: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 5: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 220 kDa, 35 kDa
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