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Anti-collagen I antibody [EPR22894-89] is a rabbit monoclonal antibody that is used in collagen alpha 1(I) chain western blot (WB) and immunoprecipitation (IP). Suitable for human, mouse and rat samples.

- Cited in 105 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR22894-89 is cited in over 170 publications
- Affinity purified using Protein A


Images

Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (AB260043), expandable thumbnail
  • Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043), expandable thumbnail
  • Western blot - Anti-Collagen I antibody [EPR22894-89] (AB260043), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (AB260043), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (AB260043), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Expected
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting.

Species

Rat

Dilution info

1/1000

Notes

Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting.

Species

Human

Dilution info

1/1000

Notes

Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab138492 can be used as a suitable replacement for ab260043 for ICC.

Species

Human

Dilution info

-

Notes

ab138492 can be used as a suitable replacement for ab260043 for ICC.

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

Alternative names

Recommended products

Anti-collagen I antibody [EPR22894-89] is a rabbit monoclonal antibody that is used in collagen alpha 1(I) chain western blot (WB) and immunoprecipitation (IP). Suitable for human, mouse and rat samples.

- Cited in 105 publications
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR22894-89 is cited in over 170 publications
- Affinity purified using Protein A

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22894-89

Purification technique

Affinity purification Protein A

Specificity

Compared with ab260043, ab255809 has lower background. We recommend ab255809 as an alternative for western blotting.

Please navigate to the support & downloads section for specificity details in Chinese.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Associated diseases and disorders

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (ab260043), expandable thumbnail

    Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Collagen I was immunoprecipitated from 0.35 mg Mouse lung lysate 10ug with ab260043 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260043 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution. Lane 1: Mouse lung lysate 10ug

    Lane 2: ab260043 IP in Mouse lung lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab260043 in Mouse lung lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

    All lanes: Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Predicted band size: 139 kDa

  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043), expandable thumbnail

    Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Procollagen I (220 kDa) and cleaved C-propeptide (35 kDa) are observed.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).

    Exposure time: Lane 1: 1 second; Lane 2: 3 seconds.

    All lanes: Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/5000 dilution

    Lane 1: HFF-1 (human skin fibroblast) whole cell lysate at 20 µg

    Lane 2: HFF-1 culture supernatant at 10 µL

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/25000 dilution

    Predicted band size: 139 kDa

    Observed band size: 220 kDa, 35 kDa

  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043), expandable thumbnail

    Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Cleaved collagen I C-propeptide (35 kDa) is observed.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311).

    Exposure time: Lanes 1-2: 3 seconds; Lane 3: 136 seconds.

    All lanes: Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) culture supernatant at 10 µL

    Lane 2: Rat lung organ culture supernatant at 10 µL

    Lane 3: Human lung tissue lysate at 10 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/25000 dilution

    Lane 3: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 139 kDa

  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

  • Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HFF-1 (Human skin fibroblast) cells labelling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

  • Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043), expandable thumbnail

    Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043)

    Blocking buffer and concentration: 5% NFDM/TBST. 

    Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure Time: ab260043: 20 seconds, Anti-Collagen I antibody [EPR22209-75] ab255809: 180 seconds.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as loading control.

    Compared with ab260043, Anti-Collagen I antibody [EPR22209-75] ab255809 has lower background. We recommend Anti-Collagen I antibody [EPR22209-75] ab255809 as an alternative for western blotting.

    For better use Anti-Collagen I antibody [EPR22209-75] ab255809, we recommend loading higher amount of lysate or using lower antibody dilution. 

    All lanes: Western blot - Anti-Collagen I antibody [EPR22894-89] (ab260043) at 1/1000 dilution

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: MDA-MB-231(Human breast adenocarcinoma epithelial cell) supernatant lysate

    Lane 4: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate

    Lane 5: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 139 kDa

    Observed band size: 220 kDa, 35 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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