AB264074

Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal COL1A1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

4 Images

Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HFF-1 (Human skin fibroblast) cells labeling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling Collagen I with ab260043 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

Unknown

Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Collagen I was immunoprecipitated from 0.35 mg mouse lung lysate 10μg with ab260043 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab260043 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : Mouse lung lysate 10μg

Lane 2 : ab260043 IP in mouse lung lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab260043 in mouse lung lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

All lanes:

Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] (<a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22894-89-ab260043'>ab260043</a>)

Predicted band size: 139 kDa

false

Lab

Western blot - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. Exposure Time : ab260043 : 20 seconds, ab255809 : 180 seconds. ab181602 was used as loading control. Compared with ab260043, ab255809 has lower background. We recommend ab255809 as an alternative for western blotting. For better use ab255809, we recommend loading higher amount of lysate or using lower antibody dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260043).

All lanes:

Western blot - Anti-Collagen I antibody [EPR22894-89] (<a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22894-89-ab260043'>ab260043</a>) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

MDA-MB-231(Human breast adenocarcinoma epithelial cell) supernatant lysate

Lane 4:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate

Lane 5:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 139 kDa

Observed band size: 220 kDa,35 kDa

false

Unconjugated

Anti-Collagen I antibody [EPR22894-89]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22894-89

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Compared with ab260043, ab255809 has lower background. We recommend ab255809 as an alternative for western blotting.

Please navigate to the Product Protocol section for specificity details in Chinese.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Compared with <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22894-89-ab260043'>ab260043</a>, <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22209-75-ab255809'>ab255809</a> has lower background. We recommend <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22209-75-ab255809'>ab255809</a> as an alternative for western blotting.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr7785-bsa-and-azide-free-ab215969'>ab215969</a> can be used as a suitable replacement for ab264074 for ICC.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Compared with <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22894-89-ab260043'>ab260043</a>, <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22209-75-ab255809'>ab255809</a> has lower background. We recommend <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22209-75-ab255809'>ab255809</a> as an alternative for western blotting.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr7785-bsa-and-azide-free-ab215969'>ab215969</a> can be used as a suitable replacement for ab264074 for ICC.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Compared with <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22894-89-ab260043'>ab260043</a>, <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22209-75-ab255809'>ab255809</a> has lower background. We recommend <a href='/en-us/products/primary-antibodies/collagen-i-antibody-epr22209-75-ab255809'>ab255809</a> as an alternative for western blotting.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

ab264074 is the carrier-free version of ab260043.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.

Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-? pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

style

left-column

Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information COL1A1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials science 13:2066-2081 PubMed40033856

2025

Hybrid lipid nanoparticles derived from human mesenchymal stem cell extracellular vesicles by microfluidic sonication for collagen I mRNA delivery to human tendon progenitor stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rubén Pareja Tello,Erwin Pavel Lamparelli,Maria Camilla Ciardulli,Jouni Hirvonen,Goncalo Barreto,Nicola Mafulli,Giovanna Della Porta,Hélder A Santos

International journal of molecular sciences 25: PubMed38542462

2024

Differential Expression of CKLF-like MARVEL Transmembrane Domain-Containing Protein 6 and Programmed Cell Death Ligand 1 as Prognostic Biomarkers in Upper Tract Urothelial Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Said Kdimati,Clemens Christoph,Änne Glass,Nadja Engel,Desiree-Louise Dräger,Claudia Maletzki,Anne-Sophie Becker,Annette Zimpfer

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Flow Cytometry (Intracellular) - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Immunoprecipitation - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Western blot - Anti-Collagen I antibody [EPR22894-89] - BSA and Azide free (AB264074)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise