Rabbit Recombinant Multiclonal COL1A1 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP, IHC-Fr and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img444648.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223)")
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img440072.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223)")
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img440071.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223)")
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img428230.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223)")
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img428231.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (AB316223)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | WB | IHC-P | ICC/IF | IP | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested | Expected |
| Mouse | Tested | Tested | Tested | Tested | Tested | Tested |
| Rat | Tested | Tested | Tested | Tested | Expected | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Negative control: HT-29. |
Species Mouse | Dilution info - | Notes Negative control: RAW 264.7. |
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Recommended products
Rabbit Recombinant Multiclonal COL1A1 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP, IHC-Fr and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1131
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316223 is the carrirer-free version of Anti-Collagen I antibody [RM1131] ab316222.
This product is a recombinant multiclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
- Biological function summary
Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.
- Pathways
Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.
- Associated diseases and disorders
Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
24 product images
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img444648.jpg "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate.
Low expression tissue: normal mouse kidney (PMID: 33758176, PMID: 29102563)
All lanes: Western blot - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/1000 dilution
Lane 1: Mouse skin tissue lysate at 20 µg
Lanes 2 and 5: Mouse kidney tissue lysate at 20 µg
Lanes 3 and 6: Mouse lung tissue lysate at 20 µg
Lanes 4 and 8: Mouse heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 139 kDa
Observed band size: 139 kDa
Exposure time: 180s
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img440072.jpg "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: RAW 264.7.
We are unsure how to define these extra bands below 100kDa.All lanes: Western blot - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/5000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 2: NIH/3T3 (mouse embryonic fibroblast) culture supernatant at 10 µL
Lane 3: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 220 kDa
Exposure time: 81s
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img440071.jpg "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HT-29.
We are unsure how to define these extra bands below 100kDa.All lanes: Western blot - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/1000 dilution
Lane 1: HFF-1 (human skin fibroblast) whole cell lysate at 20 µg
Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) culture supernatant at 10 µL
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 220 kDa
Exposure time: 180s
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img428230.jpg "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
The molecular weight observed is consistent with what has been described in the literature (PMID: 23940311)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1: 180 seconds, Lane 2: 8 seconds, Lane 3: 81 seconds
All lanes: Western blot - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/5000 dilution
Lane 1: Mouse skin tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Rat skin tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Human uterus tissue lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 139 kDa, 220 kDa, 36 kDa
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img428231.jpg "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Negative control: C6.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/1000 dilution
Lane 1: L6 (rat skeletal muscle myoblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 220 kDa, 36 kDa
Exposure time: 10s
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img428228.jpg "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Collagen I might be easily degraded. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/1000 dilution
Lane 1: HFF-1 (human skin fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 220 kDa, 36 kDa
Exposure time: 6s
![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-frozen-sections-img428306.jpg "Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skin (fresh frozen) tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining on rat skin. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-frozen-sections-img428305.jpg "Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skin (fresh frozen) tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).
Confocal image showing positive staining on mouse skin. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
![Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunoprecipitation-img428239.jpg "Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Collagen I was immunoprecipitated from 0.35 mg Mouse skin tissue lysate with Anti-Collagen I antibody [RM1131] ab316222 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Collagen I antibody [RM1131] ab316222 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse skin tissue lysate
Lane 2: Anti-Collagen I antibody [RM1131] ab316222 IP in Mouse skin tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Collagen I antibody [RM1131] ab316222 in mouse skin tissue lysateAll lanes: Immunoprecipitation - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/30 dilution
All lanes: Mouse skin tissue lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunoprecipitation-img428238.jpg "Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Collagen I was immunoprecipitated from 0.35 mg HFF-1 (human skin fibroblast) whole cell lysate with Anti-Collagen I antibody [RM1131] ab316222 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Collagen I antibody [RM1131] ab316222 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HFF-1 (human skin fibroblast) whole cell lysate
Lane 2: Anti-Collagen I antibody [RM1131] ab316222 IP in HFF-1 (human skin fibroblast) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Collagen I antibody [RM1131] ab316222 in HFF-1 whole cell lysateAll lanes: Immunoprecipitation - Anti-Collagen I antibody [RM1131] (Anti-Collagen I antibody [RM1131] ab316222) at 1/30 dilution
All lanes: HFF-1 (human skin fibroblast) whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 10s
![Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--flow-cytometry-intracellular-img428318.jpg "Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (rat glial tumor glial cell, Left) / L6 (rat skeletal muscle myoblast, Right) cells labelling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: C6.
![Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--flow-cytometry-intracellular-img428317.jpg "Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Raw 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: Raw 264.7.
![Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--flow-cytometry-intracellular-img428316.jpg "Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HT-29 (human colorectal adenocarcinoma epithelial cell, Left) / HFF-1 (human skin fibroblast, Right) cells labelling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/5000 dilution (0.01 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: HT-29.
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunocytochemistry-immunofluorescence-img428299.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/100 (5.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in NIH/3T3 cell line.
Negative control: RAW 264.7.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/500 (1ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunocytochemistry-immunofluorescence-img428298.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized L6 (rat skeletal muscle myoblast) cells labelling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/100 (5.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in L6 cell line.
Negative control: C6.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/500 (1ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunocytochemistry-immunofluorescence-img428297.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HFF-1 (human skin fibroblast) cells labelling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/100 (5.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing cytoplasmic staining in HFF-1 cell line.
Negative control: HT-29.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/500 (1ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428281.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of rat skin.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428280.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of rat stomach.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428279.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of mouse skin.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428278.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of mouse stomach.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428277.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of human breast cancer.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428276.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of human endometrium.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of human stomach.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428274.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)
This data was developed using Anti-Collagen I antibody [RM1131] ab316222, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Collagen I with Anti-Collagen I antibody [RM1131] ab316222 at 1/500 (1.012 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on stroma of human colon.
The section was incubated with Anti-Collagen I antibody [RM1131] ab316222 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--western-blot-img428228.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-frozen-sections-img428306.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-frozen-sections-img428305.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunoprecipitation-img428239.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunoprecipitation-img428238.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--flow-cytometry-intracellular-img428318.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--flow-cytometry-intracellular-img428317.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--flow-cytometry-intracellular-img428316.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunocytochemistry-immunofluorescence-img428299.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunocytochemistry-immunofluorescence-img428298.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunocytochemistry-immunofluorescence-img428297.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428281.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428280.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428279.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428278.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428277.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428276.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223), expandable thumbnail](https://content.abcam.com/products/images/collagen-i-antibody-rm1131-bsa-and-azide-free-ab316223--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428274.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I antibody [RM1131] - BSA and Azide free (ab316223)")
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