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AB317539

Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907]

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Rabbit Multiclonal COL1A1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human COL1A1.

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

ab317539 detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample : Paraffin-embedded human breast carcinoma. Collagen I + Collagen II + Collagen III stained by ab317539 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

ab317539 detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample : Paraffin-embedded mouse testis. Collagen I + Collagen II + Collagen III stained by ab317539 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

ab317539 detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis. Sample : Paraffin-embedded rat kidney. Collagen I + Collagen II + Collagen III stained by ab317539 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Western blot - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)
  • WB

Supplier Data

Western blot - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (AB317539)

U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE and the membrane was blotted with ab317539 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (ab317539) at 1/2000 dilution

Lane 1:

U87-MG whole cell extract at 30 µg

Lane 2:

U87-MG conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

HL2048 + HL1907

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human COL1A1.

P02452

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen I + II + III are major components of the extracellular matrix providing structural support to various tissues. They are sometimes referred to as fibrillar collagens due to their ability to form strong fibrillar structures. Each type consists of three polypeptide chains wound into a triple helix with type I collagen having a molecular mass around 300 kDa. Collagen I is mostly found in skin tendons and bones while collagen II is abundant in cartilage. Collagen III is primarily located in skin blood vessels and internal organs. Together they provide tensile strength and are key to tissue integrity.
Biological function summary

Collagen I + II + III support tissue resilience and elasticity enabling organs and structures to endure stress while maintaining shape. They are integral to the formation of extracellular matrix complexes and interact with other matrix proteins like elastin and fibronectin. Through these interactions they not only provide structural support but also influence cell behavior affecting processes like cell proliferation and differentiation. These collagens contribute to wound healing by forming a scaffold that facilitates cell migration and tissue remodeling.

Pathways

These collagens influence the signaling pathways involved in tissue homeostasis and repair. They are part of the transforming growth factor-beta (TGF-β) signaling pathway that manages cell growth and differentiation. Collagen binding to integrins also affects the PI3K/Akt pathway which regulates cell survival and metabolism. These interactions highlight their role in maintaining the extracellular matrix's integrity with relationships to other extracellular matrix proteins like laminins and integrins.

Abnormalities in collagen I + II + III are linked to connective tissue disorders such as osteogenesis imperfecta and Ehlers-Danlos syndrome. Osteogenesis imperfecta related to collagen I mutations results in brittle bone pathology due to impaired collagen fibril formation. In contrast mutations in collagen II are associated with cartilage disorders like Stickler syndrome affecting vision and joint function. These conditions highlight the significance of collagenous proteins in tissue stability and functionality interfacing with proteins involved in inflammatory responses and tissue repair.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information COL1A1

Additional targets

COL3A1,COL2A1
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Product promise

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