Anti-Collagen I + Collagen III antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen III antibody (AB34710)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tubuli and blood vessels labelling Collagen I and Collagen III with ab34710 at 1/15 for 1 hour at 37 °C followed by a ready to Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking : Peroxidase-Blocking Solution for 10 minutes. Substrate : DAB-Chromogen, Rabbit/Mouse. Staining/Results : Intense collagen I staining of fibres surrounding tubuli and around blood vessels. Counterstained with  hematoxylin for 15 seconds. Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen III antibody (AB34710)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human stomach mucosa (TMA) tissue labelling Collagen I and Collagen III with ab34710 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking : Peroxidase-Blocking Solution for 10 minutes. Substrate : DAB-Chromogen, Rabbit/Mouse. Staining/Results : basement membranes and blood vessels. Counterstained with hematoxylin for 15 seconds. Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen I + Collagen III antibody (AB34710)

Immunohistochemical analysis of formalin-fixed paraffin-embedded smooth muscle cells of the human stomach wall labelling Collagen I and Collagen III with ab34710 at 1/15 dilution for 1 hour at 37 °C followed by a ready to use Polymer-HRP, Rabbit/Mouse Detection Kit. Blocking : Peroxidase-Blocking Solution for 10 minutes. Substrate : DAB-Chromogen, Rabbit/Mouse. Staining/Results : smooth muscle cells surrounded by collagen fibers. Counterstained with hematoxylin for 15 seconds. Heat induced epitope retrieval (HIER) using Tris-EDTA-citrate buffer pH 7.8 for 5 minutes.

• WB

Supplier Data

Western blot - Anti-Collagen I + Collagen III antibody (AB34710)

DyLight™ 649 anti-rabbit secondary antibody at 1 : 20,000 for 30 min at RT.

Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.

Other Band(s) : Collagen Type I splice variants and isoforms.

All lanes:

Western blot - Anti-Collagen I + Collagen III antibody (ab34710) at 1/1000 dilution

All lanes:

Western blot - Native Human Collagen I protein (<a href='/en-us/products/proteins-peptides/native-human-collagen-i-protein-ab7533'>ab7533</a>) at 0.05 µg

Secondary

All lanes:

DyLight™ 649 anti-rabbit secondary antibody at 1/20000 dilution

Predicted band size: 139 kDa

false

• WB

Supplier Data

Western blot - Anti-Collagen I + Collagen III antibody (AB34710)

All lanes:

Western blot - Anti-Collagen I + Collagen III antibody (ab34710)

Lane 1:

Human Collagen I Reduced/Boiled at 10 µg

Lane 2:

Human Collagen I Reduced/Non-Boiled at 10 µg

Lane 3:

Human Collagen III Reduced/Boiled at 10 µg

Lane 4:

Human Collagen III Reduced/ Non-Boiled at 10 µg

Lane 6:

Human Collagen I Non-Reduced/Boiled at 10 µg

Lane 7:

Human Collagen I Non-Reduced/Non-Boiled at 10 µg

Lane 8:

Human Collagen III Non-Reduced/Boiled at 10 µg

Lane 9:

Human Collagen III Non-Reduced/ Non-Boiled at 10 µg

Predicted band size: 139 kDa

false

Exposure time: 8.9s