Rabbit Polyclonal Collagen II antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COL2A1 aa 50-250.
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- eLife 7:2018Lamellar projections in the endolymphatic sac act as a relief valve to regulate inner ear pressure.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIan A Swinburne et. al.PubMed 29916365
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human COL2A1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P02458
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Reactivity data
Select an application| IHC - Wmt | |
|---|---|
| Zebrafish | Tested |
IHC - Wmt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info 1/100 - 1/500 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Alternative names
Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1
Recommended products
Rabbit Polyclonal Collagen II antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human COL2A1 aa 50-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human COL2A1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link P02458
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type II also known as collagen II or native type II collagen is a fibrillar collagen essential in the formation of cartilage. It has a molecular mass of about 300 kDa. This protein is widely expressed in cartilaginous tissues including the extracellular matrix (ECM) of hyaline cartilage which makes up a significant part of the cartilage in joints and intervertebral discs. Collagen type II provides tensile strength and structural support to these tissues facilitating normal function and joint stability.
- Biological function summary
Fibrillar collagen type II is an important component of the extracellular matrix important for endochondral ossification and cartilage formation. Collagen type II forms a network that interacts with other ECM molecules such as proteoglycans and non-collagenous proteins creating a hydrated gel that resists compressive forces. This complex structure is essential for maintaining the biomechanical properties of cartilage enabling it to withstand mechanical stress and support smooth joint movements.
- Pathways
Collagen type II plays an important role in cartilage development and homeostasis. It is integral to the TGF-β signaling pathway which regulates cell growth and differentiation in chondrocytes. The protein is related to other collagens such as collagen type IX and proteoglycans like aggrecan both of which contribute to the structural integrity of cartilage. These interactions facilitate the proper assembly and organization of cartilage ECM supporting tissue function.
- Associated diseases and disorders
Collagen type II has a significant association with osteoarthritis and rheumatoid arthritis. In these degenerative conditions the degradation of collagen type II leads to cartilage breakdown and joint pain. Furthermore autoimmune response against collagen type II can occur as seen in rheumatoid arthritis where anti-collagen antibodies target the protein worsening joint inflammation. The protein's impairment or structural alteration may also involve interactions with degradative enzymes like matrix metalloproteinases exacerbating disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
IHC - Wholemount - Anti-Collagen II antibody (ab209865)
Immunofluorescent analysis of whole-mount zebrafish embryo eye labeling Collagen II (green) with ab209865 at 1/100 dilution. a-Tubulin stained Red.
IHC - Wholemount - Anti-Collagen II antibody (ab209865)
Immunohistochemical analysis of whole-mount zebrafish embryo labeling Collagen II with ab209865 at 1/200 dilution.
IHC - Wholemount - Anti-Collagen II antibody (ab209865)
Immunohistochemical analysis of whole-mount 2 days-post-fertilization zebrafish embryo labeling Collagen II with ab209865 at 1/100 dilution.
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