AB209865

Anti-Collagen II antibody

Name: Anti-Collagen II antibody (ab209865) | Abcam
Brand: Abcam Limited
SKU: AB209865

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(2 Publications)

Rabbit Polyclonal Collagen II antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human COL2A1 aa 50-250.

View Alternative Names

Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

4 Images

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

Immunofluorescent analysis of whole-mount zebrafish embryo eye labeling Collagen II (green) with ab209865 at 1/100 dilution. a-Tubulin stained Red.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

Immunohistochemical analysis of whole-mount 2 days-post-fertilization zebrafish embryo labeling Collagen II with ab209865 at 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

Col2a1a antibody detects Col2a1a protein on whole mount paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo by immunohistochemical analysis. ab209865 was used at 1/100 dilution.
Antigen Retrieval : Tris-HCl buffer, pH 9.0, 20 min at 70°C

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Human COL2A1 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

P02458

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type II also known as collagen II or native type II collagen is a fibrillar collagen essential in the formation of cartilage. It has a molecular mass of about 300 kDa. This protein is widely expressed in cartilaginous tissues including the extracellular matrix (ECM) of hyaline cartilage which makes up a significant part of the cartilage in joints and intervertebral discs. Collagen type II provides tensile strength and structural support to these tissues facilitating normal function and joint stability.

Biological function summary

Fibrillar collagen type II is an important component of the extracellular matrix important for endochondral ossification and cartilage formation. Collagen type II forms a network that interacts with other ECM molecules such as proteoglycans and non-collagenous proteins creating a hydrated gel that resists compressive forces. This complex structure is essential for maintaining the biomechanical properties of cartilage enabling it to withstand mechanical stress and support smooth joint movements.

Pathways

Collagen type II plays an important role in cartilage development and homeostasis. It is integral to the TGF-β signaling pathway which regulates cell growth and differentiation in chondrocytes. The protein is related to other collagens such as collagen type IX and proteoglycans like aggrecan both of which contribute to the structural integrity of cartilage. These interactions facilitate the proper assembly and organization of cartilage ECM supporting tissue function.

Collagen type II has a significant association with osteoarthritis and rheumatoid arthritis. In these degenerative conditions the degradation of collagen type II leads to cartilage breakdown and joint pain. Furthermore autoimmune response against collagen type II can occur as seen in rheumatoid arthritis where anti-collagen antibodies target the protein worsening joint inflammation. The protein's impairment or structural alteration may also involve interactions with degradative enzymes like matrix metalloproteinases exacerbating disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

See full target information COL2A1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 22: PubMed38393026

2024

The Characterization and Cytotoxic Evaluation of Collagen Isolated from Different Body Parts (Ectosome and Choanosome) Envisaging the Development of Biomaterials.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel S Rocha,Catarina F Marques,Ana C Carvalho,Eva Martins,Alexander Ereskovsky,Rui L Reis,Tiago H Silva

eLife 7: PubMed29916365

2018

Lamellar projections in the endolymphatic sac act as a relief valve to regulate inner ear pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Ian A Swinburne,Kishore R Mosaliganti,Srigokul Upadhyayula,Tsung-Li Liu,David G C Hildebrand,Tony Y-C Tsai,Anzhi Chen,Ebaa Al-Obeidi,Anna K Fass,Samir Malhotra,Florian Engert,Jeff W Lichtman,Tomas Kirchhausen,Eric Betzig,Sean G Megason

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen II antibody

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

IHC - Wholemount - Anti-Collagen II antibody (AB209865)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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