Anti-collagen II antibody is a rabbit polyclonal antibody that is used in collagen type 2 western blot (WB), immunoprecipitation (IP), enzyme-linked immunosorbent assay (ELISA), immunohistochemistry on paraffin-embedded sections (IHC-P), and immunocytochemistry/immunofluorescence (ICC/IF). Suitable for human, mouse, rat, chicken, cow, dog, pig and sheep samples.
- Cited in over 765 publications
- Tried and trusted by researchers since 2006
- Affinity purified
Same trusted quality, new lower price
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- Scientific reports 13:213492023A causative role for periarticular skeletal muscle weakness in the progression of joint damage and pain in OA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJu-Ryoung Kim,Thi Hong Nhung Pham,Wan-Uk Kim,Hyun Ah KimPubMed 38049482
- The American journal of pathology 194:280-2952023FOXO3-Activated HOTTIP Sequesters miR-615-3p away from COL2A1 to Mitigate Intervertebral Disc Degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingjie Hao,Guangduo Zhu,Lei Yu,Zhinan Ren,Weiwei Zhou,Panke Zhang,Xu LianPubMed 37981220
- Scientific reports 13:201682023Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShunan Yu,Xiong Shu,Lei Chen,Chao Wang,Xinyu Wang,Jinzhu Jing,Guoqiang Yan,Yanzhuo Zhang,Chengai WuPubMed 37978248
- Arthritis research & therapy 25:2172023Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/β-catenin pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Guo,Haoyan Pan,Xueying Lu,Zhong Chen,Laixi Zhou,Shuxin Chen,Jin Huang,Xinzhi Liang,Zhisheng Xiao,Hua Zeng,Yan Shao,Weizhong Qi,Denghui Xie,Chuangxin LinPubMed 37946278
- International journal of molecular sciences 24:2023Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYeji Kim,Seong Bae An,Sang-Hyuk Lee,Jong Joo Lee,Sung Bum Kim,Jae-Cheul Ahn,Dong-Youn Hwang,Inbo HanPubMed 38003216
- Nature communications 14:69092023Loss of Grem1-lineage chondrogenic progenitor cells causes osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Q Ng,Toghrul H Jafarov,Christopher B Little,Tongtong Wang,Abdullah M Ali,Yan Ma,Georgette A Radford,Laura Vrbanac,Mari Ichinose,Samuel Whittle,David J Hunter,Tamsin R M Lannagan,Nobumi Suzuki,Jarrad M Goyne,Hiroki Kobayashi,Timothy C Wang,David R Haynes,Danijela Menicanin,Stan Gronthos,Daniel L Worthley,Susan L Woods,Siddhartha MukherjeePubMed 37907525
- Materials today. Bio 23:1008302023Hypotrochoidal scaffolds for cartilage regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenny A van Kampen,Elena Olaret,Izabela-Cristina Stancu,Daniela F Duarte Campos,Horst Fischer,Carlos Mota,Lorenzo MoroniPubMed 37876709
- Nature communications 14:62572023Injectable and biodegradable piezoelectric hydrogel for osteoarthritis treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTra Vinikoor,Godwin K Dzidotor,Thinh T Le,Yang Liu,Ho-Man Kan,Srimanta Barui,Meysam T Chorsi,Eli J Curry,Emily Reinhardt,Hanzhang Wang,Parbeen Singh,Marc A Merriman,Ethan D'Orio,Jinyoung Park,Shuyang Xiao,James H Chapman,Feng Lin,Cao-Sang Truong,Somasundaram Prasadh,Lisa Chuba,Shaelyn Killoh,Seok-Woo Lee,Qian Wu,Ramaswamy M Chidambaram,Kevin W H Lo,Cato T Laurencin,Thanh D NguyenPubMed 37802985
- iScience 26:1081192023Mechanical overloading leads to chondrocyte degeneration and senescence via Zmpste24-mediated nuclear membrane instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeyu Kong,Minghao Jin,Chen Zhao,Hua Qiao,Xuzhuo Chen,Baixing Li,Kewei Rong,Pu Zhang,Yu Shan,Zhengquan Xu,Yongyun Chang,Huiwu Li,Zanjing ZhaiPubMed 37965144
- Bone & joint research 12:615-6232023Regeneration of injured articular cartilage using the recombinant human amelogenin protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmer Helwa-Shalom,Faris Saba,Elad Spitzer,Salem Hanhan,Koby Goren,Shany I Markowitz,Dekel Shilo,Nissim Khaimov,Yechiel N Gellman,Dan Deutsch,Anat Blumenfeld,Hani Nevo,Amir HazePubMed 37783468
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Full Length Protein corresponding to Human COL2A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02458
Reactivity data
Select an application| IP | ELISA | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested | Expected |
| Mouse | Expected | Expected | Expected | Predicted | Expected |
| Rat | Expected | Expected | Expected | Predicted | Expected |
| Chicken | Expected | Expected | Expected | Predicted | Expected |
| Cow | Expected | Expected | Expected | Predicted | Expected |
| Dog | Expected | Expected | Expected | Predicted | Expected |
| Mammals | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Expected | Expected | Expected | Predicted | Expected |
| Sheep | Expected | Expected | Expected | Predicted | Expected |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Species Pig | Dilution info 1/100 | Notes - |
Species Chicken | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
Species Dog | Dilution info 1/100 | Notes - |
Species Cow | Dilution info 1/100 | Notes - |
Species Sheep | Dilution info 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/5000 - 1/50000 | Notes - |
Species Pig | Dilution info 1/5000 - 1/50000 | Notes - |
Species Chicken | Dilution info 1/5000 - 1/50000 | Notes - |
Species Human | Dilution info 1/5000 - 1/50000 | Notes - |
Species Dog | Dilution info 1/5000 - 1/50000 | Notes - |
Species Cow | Dilution info 1/5000 - 1/50000 | Notes - |
Species Sheep | Dilution info 1/5000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Pig | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Chicken | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Human | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Dog | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Cow | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Sheep | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/400.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Sheep, Mammals, Mouse, Dog, Rat, Pig, Cow | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mammals | Dilution info - | Notes - |
Associated Products
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Target data
Function
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Alternative names
Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1
Recommended products
Anti-collagen II antibody is a rabbit polyclonal antibody that is used in collagen type 2 western blot (WB), immunoprecipitation (IP), enzyme-linked immunosorbent assay (ELISA), immunohistochemistry on paraffin-embedded sections (IHC-P), and immunocytochemistry/immunofluorescence (ICC/IF). Suitable for human, mouse, rat, chicken, cow, dog, pig and sheep samples.
- Cited in over 765 publications
- Tried and trusted by researchers since 2006
- Affinity purified
Same trusted quality, new lower price
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
- Full Length Protein corresponding to Human COL2A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02458
- Purification technique
- Affinity purification Immunogen
- Specificity
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
- Concentration
- Purification notes
Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Collagen II antibody (ab34712) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IHC-P, IP and WB.
Anti-Collagen II antibody (ab34712) was first used in a scientific publication in 2008 and has been cited over 766 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-Collagen II antibody (ab34712) has high sensitivity and specificity.
Anti-Collagen II antibody (ab34712) has 16 independent reviews from customers.
Anti-Collagen II antibody (ab34712) specifically detects Collagen II (UniProt ID: P02458; Molecular weight: 97kDa) and is sold in 100 ug selling sizes.
Top cited antibody for this target with >900 citations. Highly-validated collagen type II antibody, essential for researchers studying joint health and cartilage support. This antibody is crucial in osteoarthritis treatment research, particularly in understanding collagen II's benefits and sources. It is widely used in studies of collagen II supplements and ongoing collagen II research.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Collagen type II also known as collagen II or native type II collagen is a fibrillar collagen essential in the formation of cartilage. It has a molecular mass of about 300 kDa. This protein is widely expressed in cartilaginous tissues including the extracellular matrix (ECM) of hyaline cartilage which makes up a significant part of the cartilage in joints and intervertebral discs. Collagen type II provides tensile strength and structural support to these tissues facilitating normal function and joint stability.
- Biological function summary
Fibrillar collagen type II is an important component of the extracellular matrix important for endochondral ossification and cartilage formation. Collagen type II forms a network that interacts with other ECM molecules such as proteoglycans and non-collagenous proteins creating a hydrated gel that resists compressive forces. This complex structure is essential for maintaining the biomechanical properties of cartilage enabling it to withstand mechanical stress and support smooth joint movements.
- Pathways
Collagen type II plays an important role in cartilage development and homeostasis. It is integral to the TGF-β signaling pathway which regulates cell growth and differentiation in chondrocytes. The protein is related to other collagens such as collagen type IX and proteoglycans like aggrecan both of which contribute to the structural integrity of cartilage. These interactions facilitate the proper assembly and organization of cartilage ECM supporting tissue function.
- Associated diseases and disorders
Collagen type II has a significant association with osteoarthritis and rheumatoid arthritis. In these degenerative conditions the degradation of collagen type II leads to cartilage breakdown and joint pain. Furthermore autoimmune response against collagen type II can occur as seen in rheumatoid arthritis where anti-collagen antibodies target the protein worsening joint inflammation. The protein's impairment or structural alteration may also involve interactions with degradative enzymes like matrix metalloproteinases exacerbating disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Sonomoto K et al. Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold. PLoS One 11:e0153231 (2016). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712)
Formalin-fixed, paraffin-embedded human Mesenchymal stem cells stained for collagen II using ab34712 at 1/100 dilution i immunohistochemical analysis.
Healthy donor-derived MSCs were injected into the center of nano-fiber plugs and cultured in MSCGM for the specified period. The samples were assessed immunohistochemically for the expression of type-II collagen on Days 7 and 28. Scale bars, 50 μm.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712)
ab34712 (1:400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
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