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Rabbit Polyclonal Collagen II antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep samples. Cited in 767 publications. Immunogen corresponding to Full Length Protein corresponding to Human COL2A1.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Full Length Protein corresponding to Human COL2A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02458
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPELISAWBIHC-PICC/IF
Human
Expected
Expected
Expected
Tested
Expected
Mouse
Expected
Expected
Expected
Predicted
Expected
Rat
Expected
Expected
Expected
Predicted
Expected
Chicken
Expected
Expected
Expected
Predicted
Expected
Cow
Expected
Expected
Expected
Predicted
Expected
Dog
Expected
Expected
Expected
Predicted
Expected
Mammals
Predicted
Predicted
Predicted
Predicted
Predicted
Pig
Expected
Expected
Expected
Predicted
Expected
Sheep
Expected
Expected
Expected
Predicted
Expected

Expected
Expected

Species

Mouse

Dilution info

1/100

Notes

-

Species

Rat

Dilution info

1/100

Notes

-

Species

Pig

Dilution info

1/100

Notes

-

Species

Chicken

Dilution info

1/100

Notes

-

Species

Human

Dilution info

1/100

Notes

-

Species

Dog

Dilution info

1/100

Notes

-

Species

Cow

Dilution info

1/100

Notes

-

Species

Sheep

Dilution info

1/100

Notes

-

Predicted
Predicted

Species

Mammals

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/5000 - 1/50000

Notes

-

Species

Rat

Dilution info

1/5000 - 1/50000

Notes

-

Species

Pig

Dilution info

1/5000 - 1/50000

Notes

-

Species

Chicken

Dilution info

1/5000 - 1/50000

Notes

-

Species

Human

Dilution info

1/5000 - 1/50000

Notes

-

Species

Dog

Dilution info

1/5000 - 1/50000

Notes

-

Species

Cow

Dilution info

1/5000 - 1/50000

Notes

-

Species

Sheep

Dilution info

1/5000 - 1/50000

Notes

-

Predicted
Predicted

Species

Mammals

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Species

Pig

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Species

Chicken

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Species

Human

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Species

Dog

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Species

Cow

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Species

Sheep

Dilution info

1/1000 - 1/10000

Notes

This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions.

Predicted
Predicted

Species

Mammals

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50.00000 - 1/400.00000

Notes

-

Predicted
Predicted

Species

Chicken, Sheep, Mammals, Mouse, Dog, Rat, Pig, Cow

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mammals

Dilution info

-

Notes

-

Target data

Function

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

Alternative names

Recommended products

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Rabbit Polyclonal Collagen II antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep samples. Cited in 767 publications. Immunogen corresponding to Full Length Protein corresponding to Human COL2A1.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogens
  • Full Length Protein corresponding to Human COL2A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02458
Purification technique

Affinity purification Immunogen

Specificity

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

Concentration
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Purification notes

Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712), expandable thumbnail
    Sonomoto K et al. Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold. PLoS One 11:e0153231 (2016). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712)

    Formalin-fixed, paraffin-embedded human Mesenchymal stem cells stained for collagen II using ab34712 at 1/100 dilution i immunohistochemical analysis.

    Healthy donor-derived MSCs were injected into the center of nano-fiber plugs and cultured in MSCGM for the specified period. The samples were assessed immunohistochemically for the expression of type-II collagen on Days 7 and 28. Scale bars, 50 μm.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (ab34712)

    ab34712 (1:400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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