AB34712

Anti-Collagen II antibody

Name: Anti-collagen II antibody - rabbit polyclonal (ab34712) | Abcam
Brand: Abcam Limited
SKU: AB34712
Price: 525 USD
Availability: InStock
Rating: 4 (16 reviews)

(16 Reviews)

(767 Publications)

Anti-Collagen II antibody (ab34712) is a rabbit polyclonal antibody detecting Collagen II in Dot Blot, IHC-P. Suitable for Cow, Human.

- Over 760 publications
- Trusted since 2006

View Alternative Names

Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

ab34712 (1 : 400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

Formalin-fixed, paraffin-embedded human Mesenchymal stem cells stained for collagen II using ab34712 at 1/100 dilution i immunohistochemical analysis.

Healthy donor-derived MSCs were injected into the center of nano-fiber plugs and cultured in MSCGM for the specified period. The samples were assessed immunohistochemically for the expression of type-II collagen on Days 7 and 28. Scale bars, 50 μm.

Sonomoto K et al. Spontaneous Differentiation of Human Mesenchymal Stem Cells on Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold. PLoS One 11:e0153231 (2016). Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Cow

Applications

IHC-P, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Full Length Protein corresponding to Cow COL2A1. Database link P02459

Specificity

Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/400", "IHCP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Cow": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" } } }

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Product details

Anti-Collagen II antibody (ab34712) is a rabbit polyclonal antibody and is validated for use in IHC-P and Dot Blot.

Anti-Collagen II antibody (ab34712) was first used in a scientific publication in 2008 and has been cited over 766 times in peer reviewed journals. It's performance in Western blot and IHC in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-Collagen II antibody (ab34712) has high sensitivity and specificity.

Anti-Collagen II antibody (ab34712) has 16 independent reviews from customers.

Anti-Collagen II antibody (ab34712) specifically detects Collagen II (UniProt ID: P02458; Molecular weight: 97kDa) and is sold in 100 ug selling sizes.

Top cited antibody for this target with >900 citations. Highly-validated collagen type II antibody, essential for researchers studying joint health and cartilage support. This antibody is crucial in osteoarthritis treatment research, particularly in understanding collagen II's benefits and sources. It is widely used in studies of collagen II supplements and ongoing collagen II research.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type II also known as collagen II or native type II collagen is a fibrillar collagen essential in the formation of cartilage. It has a molecular mass of about 300 kDa. This protein is widely expressed in cartilaginous tissues including the extracellular matrix (ECM) of hyaline cartilage which makes up a significant part of the cartilage in joints and intervertebral discs. Collagen type II provides tensile strength and structural support to these tissues facilitating normal function and joint stability.

Biological function summary

Fibrillar collagen type II is an important component of the extracellular matrix important for endochondral ossification and cartilage formation. Collagen type II forms a network that interacts with other ECM molecules such as proteoglycans and non-collagenous proteins creating a hydrated gel that resists compressive forces. This complex structure is essential for maintaining the biomechanical properties of cartilage enabling it to withstand mechanical stress and support smooth joint movements.

Pathways

Collagen type II plays an important role in cartilage development and homeostasis. It is integral to the TGF-β signaling pathway which regulates cell growth and differentiation in chondrocytes. The protein is related to other collagens such as collagen type IX and proteoglycans like aggrecan both of which contribute to the structural integrity of cartilage. These interactions facilitate the proper assembly and organization of cartilage ECM supporting tissue function.

Collagen type II has a significant association with osteoarthritis and rheumatoid arthritis. In these degenerative conditions the degradation of collagen type II leads to cartilage breakdown and joint pain. Furthermore autoimmune response against collagen type II can occur as seen in rheumatoid arthritis where anti-collagen antibodies target the protein worsening joint inflammation. The protein's impairment or structural alteration may also involve interactions with degradative enzymes like matrix metalloproteinases exacerbating disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

See full target information COL2A1

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Publications (767)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:21349 PubMed38049482

2023

A causative role for periarticular skeletal muscle weakness in the progression of joint damage and pain in OA.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Ryoung Kim,Thi Hong Nhung Pham,Wan-Uk Kim,Hyun Ah Kim

The American journal of pathology 194:280-295 PubMed37981220

2023

FOXO3-Activated HOTTIP Sequesters miR-615-3p away from COL2A1 to Mitigate Intervertebral Disc Degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Hao,Guangduo Zhu,Lei Yu,Zhinan Ren,Weiwei Zhou,Panke Zhang,Xu Lian

Scientific reports 13:20168 PubMed37978248

2023

Construction of ultrasonically treated collagen/silk fibroin composite scaffolds to induce cartilage regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shunan Yu,Xiong Shu,Lei Chen,Chao Wang,Xinyu Wang,Jinzhu Jing,Guoqiang Yan,Yanzhuo Zhang,Chengai Wu

Arthritis research & therapy 25:217 PubMed37946278

2023

Rspo2 exacerbates rheumatoid arthritis by targeting aggressive phenotype of fibroblast-like synoviocytes and disrupting chondrocyte homeostasis via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Guo,Haoyan Pan,Xueying Lu,Zhong Chen,Laixi Zhou,Shuxin Chen,Jin Huang,Xinzhi Liang,Zhisheng Xiao,Hua Zeng,Yan Shao,Weizhong Qi,Denghui Xie,Chuangxin Lin

International journal of molecular sciences 24: PubMed38003216

2023

Enhanced Intervertebral Disc Repair via Genetically Engineered Mesenchymal Stem Cells with Tetracycline Regulatory System.

Applications

Unspecified application

Species

Unspecified reactive species

Yeji Kim,Seong Bae An,Sang-Hyuk Lee,Jong Joo Lee,Sung Bum Kim,Jae-Cheul Ahn,Dong-Youn Hwang,Inbo Han

Nature communications 14:6909 PubMed37907525

2023

Loss of Grem1-lineage chondrogenic progenitor cells causes osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Q Ng,Toghrul H Jafarov,Christopher B Little,Tongtong Wang,Abdullah M Ali,Yan Ma,Georgette A Radford,Laura Vrbanac,Mari Ichinose,Samuel Whittle,David J Hunter,Tamsin R M Lannagan,Nobumi Suzuki,Jarrad M Goyne,Hiroki Kobayashi,Timothy C Wang,David R Haynes,Danijela Menicanin,Stan Gronthos,Daniel L Worthley,Susan L Woods,Siddhartha Mukherjee

Materials today. Bio 23:100830 PubMed37876709

2023

Hypotrochoidal scaffolds for cartilage regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Kenny A van Kampen,Elena Olaret,Izabela-Cristina Stancu,Daniela F Duarte Campos,Horst Fischer,Carlos Mota,Lorenzo Moroni

Nature communications 14:6257 PubMed37802985

2023

Injectable and biodegradable piezoelectric hydrogel for osteoarthritis treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Tra Vinikoor,Godwin K Dzidotor,Thinh T Le,Yang Liu,Ho-Man Kan,Srimanta Barui,Meysam T Chorsi,Eli J Curry,Emily Reinhardt,Hanzhang Wang,Parbeen Singh,Marc A Merriman,Ethan D'Orio,Jinyoung Park,Shuyang Xiao,James H Chapman,Feng Lin,Cao-Sang Truong,Somasundaram Prasadh,Lisa Chuba,Shaelyn Killoh,Seok-Woo Lee,Qian Wu,Ramaswamy M Chidambaram,Kevin W H Lo,Cato T Laurencin,Thanh D Nguyen

iScience 26:108119 PubMed37965144

2023

Mechanical overloading leads to chondrocyte degeneration and senescence via Zmpste24-mediated nuclear membrane instability.

Applications

Unspecified application

Species

Unspecified reactive species

Keyu Kong,Minghao Jin,Chen Zhao,Hua Qiao,Xuzhuo Chen,Baixing Li,Kewei Rong,Pu Zhang,Yu Shan,Zhengquan Xu,Yongyun Chang,Huiwu Li,Zanjing Zhai

Bone & joint research 12:615-623 PubMed37783468

2023

Regeneration of injured articular cartilage using the recombinant human amelogenin protein.

Applications

Unspecified application

Species

Unspecified reactive species

Omer Helwa-Shalom,Faris Saba,Elad Spitzer,Salem Hanhan,Koby Goren,Shany I Markowitz,Dekel Shilo,Nissim Khaimov,Yechiel N Gellman,Dan Deutsch,Anat Blumenfeld,Hani Nevo,Amir Haze

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen II antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen II antibody (AB34712)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (767)

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