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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal Collagen II antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep samples. Cited in 767 publications. Immunogen corresponding to Full Length Protein corresponding to Human COL2A1.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
IP | ELISA | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Expected | Expected | Expected | Tested | Expected |
Mouse | Expected | Expected | Expected | Predicted | Expected |
Rat | Expected | Expected | Expected | Predicted | Expected |
Chicken | Expected | Expected | Expected | Predicted | Expected |
Cow | Expected | Expected | Expected | Predicted | Expected |
Dog | Expected | Expected | Expected | Predicted | Expected |
Mammals | Predicted | Predicted | Predicted | Predicted | Predicted |
Pig | Expected | Expected | Expected | Predicted | Expected |
Sheep | Expected | Expected | Expected | Predicted | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
Species Rat | Dilution info 1/100 | Notes - |
Species Pig | Dilution info 1/100 | Notes - |
Species Chicken | Dilution info 1/100 | Notes - |
Species Human | Dilution info 1/100 | Notes - |
Species Dog | Dilution info 1/100 | Notes - |
Species Cow | Dilution info 1/100 | Notes - |
Species Sheep | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mammals | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/5000 - 1/50000 | Notes - |
Species Rat | Dilution info 1/5000 - 1/50000 | Notes - |
Species Pig | Dilution info 1/5000 - 1/50000 | Notes - |
Species Chicken | Dilution info 1/5000 - 1/50000 | Notes - |
Species Human | Dilution info 1/5000 - 1/50000 | Notes - |
Species Dog | Dilution info 1/5000 - 1/50000 | Notes - |
Species Cow | Dilution info 1/5000 - 1/50000 | Notes - |
Species Sheep | Dilution info 1/5000 - 1/50000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mammals | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Pig | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Chicken | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Human | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Dog | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Cow | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species Sheep | Dilution info 1/1000 - 1/10000 | Notes This product is not recommended for use under denaturing conditions in WB, IP, and ELISA. We would suggest testing it under native conditions. |
Species | Dilution info | Notes |
---|---|---|
Species Mammals | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50.00000 - 1/400.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chicken, Sheep, Mammals, Mouse, Dog, Rat, Pig, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mammals | Dilution info - | Notes - |
Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.
Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1
Rabbit Polyclonal Collagen II antibody. Suitable for IP, ELISA, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Pig, Chicken, Human, Dog, Cow, Sheep samples. Cited in 767 publications. Immunogen corresponding to Full Length Protein corresponding to Human COL2A1.
Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
Affinity purification Immunogen
Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens.
Blue Ice
1-2 weeks
+4°C
-20°C
Add glycerol to a final volume of 50% for extra stability and aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Formalin-fixed, paraffin-embedded human Mesenchymal stem cells stained for collagen II using ab34712 at 1/100 dilution i immunohistochemical analysis.
Healthy donor-derived MSCs were injected into the center of nano-fiber plugs and cultured in MSCGM for the specified period. The samples were assessed immunohistochemically for the expression of type-II collagen on Days 7 and 28. Scale bars, 50 μm.
ab34712 (1:400, 45 min RT) showed moderate staining in FFPE human bronchiolar cartilage. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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