AB188570

Anti-Collagen II antibody [EPR12268]

Name: Anti-Collagen II antibody [EPR12268] Rabbit monoclonal (ab188570) | Abcam
Brand: Abcam Limited
SKU: AB188570
Price: 320 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(135 Publications)

Rabbit Recombinant Monoclonal Collagen II antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 135 publications.

View Alternative Names

Collagen alpha-1(II) chain, Alpha-1 type II collagen, COL2A1

3 Images

Supplier Data

Western blot - Anti-Collagen II antibody [EPR12268] (AB188570)

Actual band size : 30 kDa (possible isoform or fragment).

Negative control : liver (PMID : 31553813, PMID : 9121483).

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Collagen II antibody [EPR12268] (ab188570) at 1/5000 dilution

Lane 1:

Rat articular cartilage lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Collagen II antibody [EPR12268] (AB188570)

Actual band size : 36kDa (His-tagged recombinant fragment 1242-1487 aa purified from HEK293 cells)

All lanes:

Western blot - Anti-Collagen II antibody [EPR12268] (ab188570) at 1/1000 dilution

All lanes:

Human Collagen II recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 141 kDa

Observed band size: 36 kDa

false

Lab

Western blot - Anti-Collagen II antibody [EPR12268] (AB188570)

Actual band size : 30kDa (possible isoform or fragment) Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Collagen II antibody [EPR12268] (ab188570) at 1/5000 dilution

Lane 1:

Rat cartilage lysate at 20 µg

Lane 2:

Human cartilage lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 141 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12268

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type II also known as collagen II or native type II collagen is a fibrillar collagen essential in the formation of cartilage. It has a molecular mass of about 300 kDa. This protein is widely expressed in cartilaginous tissues including the extracellular matrix (ECM) of hyaline cartilage which makes up a significant part of the cartilage in joints and intervertebral discs. Collagen type II provides tensile strength and structural support to these tissues facilitating normal function and joint stability.

Biological function summary

Fibrillar collagen type II is an important component of the extracellular matrix important for endochondral ossification and cartilage formation. Collagen type II forms a network that interacts with other ECM molecules such as proteoglycans and non-collagenous proteins creating a hydrated gel that resists compressive forces. This complex structure is essential for maintaining the biomechanical properties of cartilage enabling it to withstand mechanical stress and support smooth joint movements.

Pathways

Collagen type II plays an important role in cartilage development and homeostasis. It is integral to the TGF-β signaling pathway which regulates cell growth and differentiation in chondrocytes. The protein is related to other collagens such as collagen type IX and proteoglycans like aggrecan both of which contribute to the structural integrity of cartilage. These interactions facilitate the proper assembly and organization of cartilage ECM supporting tissue function.

Collagen type II has a significant association with osteoarthritis and rheumatoid arthritis. In these degenerative conditions the degradation of collagen type II leads to cartilage breakdown and joint pain. Furthermore autoimmune response against collagen type II can occur as seen in rheumatoid arthritis where anti-collagen antibodies target the protein worsening joint inflammation. The protein's impairment or structural alteration may also involve interactions with degradative enzymes like matrix metalloproteinases exacerbating disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.

See full target information COL2A1

style

left-column

Publications (135)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 35:e2506035 PubMed41016815

2025

Functional Benefits of Low-Molecular-Weight Collagen Peptide in Achilles Tendon and Medial Collateral Ligament Injuries and Anterior Cruciate Ligament Transection-Induced Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Eunjung Choi,Myunghee Kim,Dae-Hyo Lee,Sehwan Shim,Do-Un Kim,Jeong-Min Hong,Hee-Chul Chung

Innovation (Cambridge (Mass.)) 6:100913 PubMed40697780

2025

Trojan horse-inspired spatiotemporal strategy augments cartilage regeneration by enhancing mitochondrial energy production.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Lu,Yang Liu,Xiaowei Xia,Yubin Wu,Yaoge Deng,Chenqi Yu,Jianfeng Yu,Mingzhuang Hou,Huilin Yang,Yijian Zhang,Xuesong Zhu

Journal of orthopaedic translation 53:187-205 PubMed40678612

2025

PPARγ controls ESCRT-dependent fibroblast-like synoviocyte exosome biogenesis and alleviates chondrocyte osteoarthritis mediated by exosomal ANXA1.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangshuo Jia,Yue Yang,Jiabao Liu,Ziyuan Wang,Lunhao Bai

Journal of orthopaedic translation 52:220-232 PubMed40337550

2025

Therapeutic effect of edaravone on osteoarthritis: targeting NRF2 signaling and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Jiang,Yuhang Gong,Xinyu Wu,Jiangjie Chen,Yiyu Chen,Jingyao Chen,Fang Tang,Zhiyu Fang,Yuxuan Bao,Jiajing Ye,Zhangfu Wang,Zhenghua Hong

Molecular medicine (Cambridge, Mass.) 31:128 PubMed40197110

2025

S-propargyl-cysteine attenuates temporomandibular joint osteoarthritis by regulating macrophage polarization via Inhibition of JAK/STAT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyi Cai,Antong Wu,Zhongxiao Lin,Wei Cao,Janak L Pathak,Richard T Jaspers,Rui Li,Xin Li,Kaihan Zheng,Yufu Lin,Na Zhou,Xin Zhang,Yizhun Zhu,Qingbin Zhang

American journal of translational research 17:75-86 PubMed39959196

2025

Establishment and evaluation of a rat model of cervical spondylosis with Yin deficiency syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Wenlong Yang,Muqing Liu,Fangming Liu

Journal of biochemical and molecular toxicology 38:e70064 PubMed39569601

2024

Extracellular Vesicles Derived Ectonucleoside Triphosphate Diphosphohydrolase 3 Alleviates Mitochondrial Dysfunction of Osteoarthritis Chondrocytes via Ectonucleotide Pyrophosphatase/Phosphodiesterase 1-Induced Suppression of the AKT/Notch2 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tang,Jingsheng He,Ye Hao

Journal of nanobiotechnology 22:634 PubMed39420311

2024

Selenium nanoparticles ameliorate lumbar disc degeneration by restoring GPX1-mediated redox homeostasis and mitochondrial function of nucleus pulposus cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wei He,Xin Tian,Quan Zhou,Jiaheng Lv,Yangfeng Li,Chenyang Jin,Hao Liu,Huiling Yang,Yong Xu,Fan He,Tao Liu

Journal of cellular and molecular medicine 28:e70027 PubMed39159149

2024

PLCγ1 deficiency in chondrocytes accelerates the age-related changes in articular cartilage and subchondral bone.

Applications

Unspecified application

Species

Unspecified reactive species

Qiubo Zhao,Xiaolei Chen,Ning Qu,Jinhua Qiu,Bing Zhang,Chun Xia

Science advances 10:eado7249 PubMed39151007

2024

Magnetically attracting hydrogel reshapes iron metabolism for tissue repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yichang Xu,Feng Cai,Yidi Zhou,Jincheng Tang,Jiannan Mao,Wei Wang,Ziang Li,Liang Zhou,Yu Feng,Kun Xi,Yong Gu,Liang Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Collagen II antibody [EPR12268]

Western blot - Anti-Collagen II antibody [EPR12268] (AB188570)

Western blot - Anti-Collagen II antibody [EPR12268] (AB188570)

Western blot - Anti-Collagen II antibody [EPR12268] (AB188570)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (135)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise